Cold-adapted protease derived from pseudoalteromonas arctica pamc 21717 and uses thereof

ABSTRACT

A cold-adapted protease derived from  Pseudoalteromonas arctica  PAMC 21717 or a recombinant cold-adapted protease obtained by expressing a gene encoding the cold-adapted protease in  E. coli  is described, and more particularly, a crystal of a protease exhibiting activity at low temperatures, a method for crystallizing the protease, a method for preparing the protease, a recombinant microorganism that expresses the protease, a method for preparing the recombinant microorganism, a method for preparing the recombinant protease and the use of the cold-adapted protease. The cold-adapted protease exhibits high activity at low temperatures, and securely maintains its enzymatic activity even in the presence of high pH, various metal ions and surfactants. Thus, it is useful in various industrial applications.

CROSS-REFERENCE TO RELATED APPLICATION

This application claims priority under the provisions of 35 USC 119 to Korean Patent Application No. 10-2014-0127957 filed Sep. 24, 2014. The disclosure of Korean Patent Application No. 10-2014-0127957 is hereby incorporated herein by reference, in its entirety, for all purposes.

TECHNICAL FIELD

The present invention relates to a cold-adapted protease derived from Pseudoalteromonas arctica PAMC 21717 or a recombinant cold-adapted protease obtained by expressing a gene encoding the cold-adapted protease in E. coli, and more particularly, to a crystal of a protease exhibiting activity at low temperatures, a method for crystallizing the protease, a method for preparing the protease, a recombinant microorganism that expresses the protease, a method for preparing the recombinant microorganism, a method for preparing the recombinant protease and the use of the cold-adapted protease.

BACKGROUND ART

Proteases are used in various applications, including pharmaceutical compositions such as digestive enzyme agents for alleviating gastrointestinal disorders, thrombolytic agents, or anti-inflammatory agents, and compositions for clothes, contact lenses or cleaners, as well as cosmetics, leather processing agents, food softeners, meat enhancers, feed of food additives, oil and fat separating agents, wastewater treatment, etc. The utility of enzymes among microbial products is already widely known. Proteases account for the highest percentage (60% or higher) of the industrial enzyme market, and the marketability thereof is more and more increasing.

About 25% of industrial proteases are marketed as detergents, and proteases for detergents are required to have a wide spectrum of substrate specificity capable of degrading food, blood or body fluid components, and an alkaline environment, and should be stable so that they do not lose their surfactant activity and enzymatic activity at high temperatures or low temperatures. In the past, plant-derived proteases were mainly used, but in recent years, microorganisms have been most frequently used to produce proteases.

If proteases are to be used for industrial applications, they are required to be very stable. For example, the activity of most proteins decreases or completely disappears in the presence of surfactants, and for this reason, proteases that are used in detergents are extremely limited. Also, if detergents are used at high concentrations, the activity of proteases contained therein is difficult to expect. In addition, conditions such as exposure to extreme pH, exposure to heavy metals, or the degree of oxidation-reduction, all strongly influence the activity of enzymes, and thus if these conditions are out of suitable ranges, enzymes rapidly lose their activity. For this reason, in order for proteases to be regularly used for industrial applications, the proteases are required to maintain their activity even under extreme and unstable physical and chemical conditions.

Serine-based proteases such as subtilisin have been most widely used in the detergent industry. Such basic proteases securely maintain their activity even under high pH conditions in which a surfactant and an oxidizing agent are present (Gupta et al., Appl Microbiol Biotechnol, 59:15-32, 2002; Haddar et al., Bioresour Technol, 100:3366-3373, 2009), and thus are useful in various industrial applications.

In recent years, a need for cold-active enzymes has increased. Particularly, low-active proteases have been added to detergents, and maintain their high activity at a laundry temperature of 15° C. or lower.

In documents regarding cold-adapted proteases, reported to date, there are reports of the cloning of serine-based cold-active protease genes derived from the psychrophilic microorganism Shewanella, the purification and characterization of enzymes (Kulakova et al., Appl Environ Microbiol, 65:611-617, 1999), the purification, characterization and sequencing (Huston et al., Appl Environ Microbiol, 70:3321-3328, 2004) of the psychrophilic marine microorganism Colwellia psychrerythraea, the purification and characterization of cold-adapted serine-based proteases derived from Colwellia sp., (Wang et al., Biotechnol Lett, 27:1195-1198, 2005) and the like.

Accordingly, the present inventors have made extensive efforts to develop proteases, which are used in various industrial applications, by using a cold-adapted and basic serine-based protease produced from Pseudoalteromonas arctica PAMC 21717 isolated from Antarctic Ocean sediments. As a result, the present inventors have isolated a cold-adapted protease that exhibits enzymatic activity under the conditions of 0 to 60° C. and pH 5.0 to 11.0 and then have identified the crystalline structure of the cold-adapted protease, and have found that the cold-adapted protease exhibits high activity at relatively low temperatures compared to proteases such as subtilisin, thereby completing the present invention.

DISCLOSURE OF INVENTION

It is an object of the present invention to provide a crystal of either a cold-adapted protease derived from Pseudoalteromonas arctica PAMC 21717 or a recombinant cold-adapted protease obtained by expressing a gene encoding the cold-adapted protease in E. coli, and a method for crystallizing the cold-adapted protease.

Another object of the present invention is to provide a method for preparing the cold-adapted protease.

Still another object of the present invention is to provide an enzyme-producing recombinant microorganism that expresses the cold-adapted protease, and a method for preparing the recombinant microorganism.

Yet another object of the present invention is to provide a method for preparing the cold-adapted protease using the recombinant microorganism.

A further object of the present invention is to provide a disinfectant composition for a surgical or therapeutic device, a detergent composition, a feed additive composition, a food additive composition, and a fiber or leather processing composition, the composition containing the cold-adapted protease as an active ingredient.

To achieve the above objects, the present invention provides a crystal of either a cold-adapted protease derived from Pseudoalteromonas arctica PAMC 21717 or a recombinant cold-adapted protease obtained by expressing a gene encoding the cold-adapted protease in E. coli, wherein the crystal of the cold-adapted protease has the following characteristics: (i) consisting of a subtilisin-like fold; (ii) having four calcium ions and two disulfide bonds (Cys439-Cys442 and Cys207-Cys254); (iii) forming a three-dimensional structure consists ten α-helices which surround the central 6 β-strands and two β-strands; and (iv) having a structure crystallized with P2₁2₁2₁ space group either unit cell parameters of a=47.9 Å, b=74.6 Å, c=83.0 Å, α=β=γ 90° or 2374 atomic coordinates (including 4 calcium ions) set forth in Table 1.

The present invention also a method for crystallizing a cold-adapted protease derived from Pseudoalteromonas arctica PAMC 21717 or a recombinant cold-adapted protease obtained by expressing a gene encoding the cold-adapted protease in E. coli, wherein the method comprises crystallizing at 20° C. using a protein solution containing 20 mM Tris-HCl (pH 8.0) and 150 mM NaCl, and a preservative solution containing 0.1 M sodium acetate (pH 4.4) and 3 M sodium chloride.

The present invention also provides a method for preparing a cold-adapted protease, the method comprises: (a) fed-batch-culturing Pseudoalteromonas arctica PAMC 21717 in a medium containing skim milk, tryptone, Fe(C₆H₅O₇), NaCl, MgCl₂, Na₂SO₄, CaCl₂, NaHCO₃ and KBr, to produce a cold-adapted protease (W-pro21717); and (b) recovering the produced cold-adapted protease.

The present invention also provides an enzyme-producing recombinant microorganism, in which a recombinant vector containing an amplification product obtained by PCR using a set of primers of SEQ ID NOs: 7 and 8 is introduced.

The present invention also provides a method for preparing a cold-adapted protease, the method comprises: (a) culturing the above-described recombinant microorganism in a medium containing glucose, KH₂PO₄, (NH₄)₂PO₄, citric acid, MgSO₄7H₂O, thiamine, an antibiotic and a trace metal element; (b) expressing a cold-adapted protease in the recombinant microorganism to a pH-stat batch culture while supplying a predetermined amount of a medium containing glucose, a yeast extract, (NH₄)₂PO₄, MgSO₄7H₂O and an antibiotic when the pH and DO of the medium is increased; and (c) recovering the expressed cold-adapted protease.

The present invention also provides a disinfectant composition for a surgical or therapeutic device, the composition containing the cold-adapted protease or a crystal of the cold-adapted protease as an active ingredient.

The present invention also provides a detergent composition containing the cold-adapted protease or a crystal of the cold-adapted protease as an active ingredient.

The present invention also provides a feed additive composition containing the cold-adapted protease or a crystal of the cold-adapted protease as an active ingredient.

The present invention also provides a food additive composition containing the cold-adapted protease or a crystal of the cold-adapted protease as an active ingredient.

The present invention also provides a fiber or leather processing composition containing the cold-adapted protease or a crystal of the cold-adapted protease as an active ingredient.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 shows the results of zymogram analysis of a culture of Pseudoalteromonas arctica PAMC 21717. (A): time-dependent analysis of the culture by zymogram; and (B) W-Pro21717 partially isolated from a 96-hr culture.

FIG. 2 shows the results of analyzing the activity and stability of W-Pro21717 as a function of temperature and pH. (A): a temperature optimal for the activity of W-Pro21717; (B) a temperature optimal for the stability of W-Pro21717; (C) a pH optimal for the activity of W-Pro21717; (D): a pH optimal for the stability of W-Pro21717.

FIG. 3 shows the results of analyzing the activity and stability of W-Pro21717 in the presence of metal ions and surfactants.

FIG. 4 shows the results of analyzing the compatibility of W-Pro21717 with a commercial protease using skim milk.

FIG. 5 shows the results of measuring the concentration of cells and the activity of W-Pro21717 after 3 hours of culture in order to examine the effects of carbon and nitrogen on medium optimization. (A): the effect of a carbon source on a skim milk-containing medium; (B) the effect of a carbon source on a medium containing no skim milk (A: control, B: glucose, C: galactose, D: fructose, E: lactose, F: sucrose, G: maltose, H: glycerol, I: starch, J: cellulose); and (C): the effect of nitrogen on a skim milk-containing medium (A: control, B: peptone, C: tryptone, D: yeast extract, E: soy peptone, F: tryptic soy broth)

FIG. 6 depicts three-dimensional reaction plots showing the effects of minerals on the optimization of production medium. (A): Fe(C₆H₅O₇) and NaCl; (B): Fe(C₆H₅O₇) and Na₂SO₄; (C): Fe(C₆H₅O₇) and KCl; (D): NaCl and Na₂SO₄; (E) NaCl and KCl; and (F) Na₂SO₄ and KCl.

FIG. 7 shows the time-dependent activity of W-Pro21717 in a fed-batch culture process. (A) : basal medium containing no skim milk, ▪: skim milk-containing basal medium, ▴: optimized mineral medium; (B) ⋄: cell concentration, ♦: W-Pro21717 activity.

FIG. 8 shows the results of SDS-PAGE and zymogram analysis of recombinant protease R-Pro21717. (A): a recombinant protein expressed from a pDOC vector; (B) a recombinant protein during a refolding process; (C) recombinant protease R-Pro21717 purified after refolding. (1 to 5: inclusion bodies after washing of pDOC122, pDOC125, pDOC128, pDOC131 and pDOC131, respectively, 6: pDOC131 after 48 hours of refolding, 7: pDOC131 after 72 hours of refolding, 8: the expression product R-Pro21717 of pDOC131 purified by chromatography after refolding).

FIG. 9 shows the results of analyzing the activity and stability of recombinant R-Pro21717 as a function of temperature and pH. (A): a temperature optimal for the activity of R-Pro21717; (B): a temperature optimal for the stability of R-Pro21717; (C): a pH optimal for the activity of R-Pro21717; and (D): a pH optimal for the stability of R-Pro21717.

FIG. 10 shows the results of analyzing the activity and stability of recombinant R-Pro21717 in the presence of metal ions and surfactants. The activity was measured after adding R-Pro21717 to a standard buffer containing 1 mM of each material, and then allowing the R-Pro21717 on ice for 1 hour.

FIG. 11 shows the three-dimensional crystalline structure of a cold-adapted protease (R-Pro21717) that has four calcium ion bonds and an active site consisting of a catalytic triad composed of Asp185, His244 and Ser425.

BEST MODE FOR CARRYING OUT THE INVENTION

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the invention pertains. Generally, the nomenclature used herein and the experiment methods are those well known and commonly employed in the art.

In the present invention, the three-dimensional crystalline structure and active site of a cold-adapted, basic serine-based protease prepared from Pseudoalteromonas arctica PAMC 21717 isolated from Antarctic Ocean sediments were examined, and the characteristics of the protease were analyzed. Pseudoalteromonas arctica PAMC 21717 that is used in the present invention was deposited under accession number KCTC 12482BP on Sep. 3, 2013.

In one aspect, the present invention is directed to a crystal of a cold-adapted protease derived from Pseudoalteromonas arctica PAMC 21717 or a recombinant cold-adapted protease obtained by expressing a gene encoding the cold-adapted protease in E. coli, wherein the crystal of the cold-adapted protease has the following characteristics: (i) consisting of a subtilisin-like fold; (ii) having four calcium ions and two disulfide bonds (Cys439-Cys442 and Cys207-Cys254); (iii) forming a three-dimensional structure consists ten α-helices which surround the central 6 β-strands and two β-strands; and (iv) having a structure crystallized with P2₁2₁2₁ space group either unit cell parameters of a=47.9 Å, b=74.6 Å, c=83.0 Å, α=β=γ 90° or 2374 atomic coordinates (including 4 calcium ions) set forth in Table 1.

In the present invention, a crystal of the protease comprises an enzymatic active site consisting of a catalytic triad of amino acid residues Asp185, His244 and Ser425.

The three-dimensional crystalline structure of the cold-adapted protease of the present invention can be represented by the atomic coordinates shown in Table 1 below.

TABLE 1 A B C D E F G H I J K ATOM 1 N SER A 119 29.662 −2.222 −19.296 1.00 13.97 ATOM 2 CA SER A 119 30.116 −2.772 −18.080 1.00 13.32 ATOM 3 CB SER A 119 30.331 −1.704 −17.074 1.00 17.13 ATOM 4 OG SER A 119 29.203 −0.888 −16.922 1.00 19.57 ATOM 5 C SER A 119 29.162 −3.752 −17.528 1.00 12.74 ATOM 6 O SER A 119 27.947 −3.671 −17.841 1.00 15.90 ATOM 7 N THR A 120 29.576 −4.689 −16.733 1.00 13.01 ATOM 8 CA THR A 120 28.740 −5.705 −16.131 1.00 16.10 ATOM 9 CB THR A 120 29.089 −7.146 −16.626 1.00 22.08 ATOM 10 OG1 THR A 120 28.606 −7.278 −18.004 1.00 29.55 ATOM 11 CG2 THR A 120 28.472 −8.209 −15.807 1.00 25.79 ATOM 12 C THR A 120 28.911 −5.565 −14.663 1.00 14.48 ATOM 13 O THR A 120 30.046 −5.623 −14.148 1.00 15.53 ATOM 14 N PRO A 121 27.859 −5.374 −13.898 1.00 11.10 ATOM 15 CA PRO A 121 28.025 −5.280 −12.462 1.00 11.72 ATOM 16 CB PRO A 121 26.760 −4.634 −12.002 1.00 13.83 ATOM 17 CG PRO A 121 25.777 −5.118 −12.949 1.00 13.25 ATOM 18 CD PRO A 121 26.460 −5.183 −14.325 1.00 12.56 ATOM 19 C PRO A 121 28.339 −6.556 −11.767 1.00 10.34 ATOM 20 O PRO A 121 28.139 −7.601 −12.301 1.00 10.98 ATOM 21 N ASN A 122 28.767 −6.391 −10.527 1.00 10.08 ATOM 22 CA ASN A 122 29.170 −7.539 −9.760 1.00 10.51 ATOM 23 CB ASN A 122 30.378 −7.156 −8.856 1.00 11.45 ATOM 24 CG ASN A 122 29.991 −6.361 −7.692 1.00 11.94 ATOM 25 OD1 ASN A 122 28.812 −6.148 −7.319 1.00 12.06 ATOM 26 ND2 ASN A 122 31.000 −6.035 −6.884 1.00 16.58 ATOM 27 C ASN A 122 28.132 −8.246 −8.912 1.00 10.10 ATOM 28 O ASN A 122 28.315 −9.238 −8.258 1.00 12.28 ATOM 29 N ASP A 123 26.863 −7.783 −9.066 1.00 9.30 ATOM 30 CA ASP A 123 25.823 −8.343 −8.254 1.00 9.23 ATOM 31 CB ASP A 123 24.538 −7.490 −8.474 1.00 9.04 ATOM 32 CG ASP A 123 24.792 −6.042 −8.214 1.00 7.86 ATOM 33 OD1 ASP A 123 25.386 −5.415 −9.101 1.00 8.84 ATOM 34 OD2 ASP A 123 24.470 −5.487 −7.146 1.00 8.40 ATOM 35 C ASP A 123 25.609 −9.820 −8.560 1.00 8.58 ATOM 36 O ASP A 123 25.495 −10.146 −9.733 1.00 9.49 ATOM 37 N PRO A 124 25.485 −10.661 −7.524 1.00 7.89 ATOM 38 CA PRO A 124 25.526 −12.092 −7.784 1.00 9.00 ATOM 39 CB PRO A 124 25.659 −12.698 −6.398 1.00 9.56 ATOM 40 CG PRO A 124 25.143 −11.583 −5.470 1.00 10.57 ATOM 41 CD PRO A 124 25.591 −10.329 −6.088 1.00 8.74 ATOM 42 C PRO A 124 24.335 −12.604 −8.533 1.00 10.01 ATOM 43 O PRO A 124 24.378 −13.674 −9.148 1.00 12.77 ATOM 44 N ARG A 125 23.175 −11.931 −8.452 1.00 8.76 ATOM 45 CA ARG A 125 22.010 −12.345 −9.220 1.00 8.50 ATOM 46 CB ARG A 125 20.733 −12.113 −8.414 1.00 8.44 ATOM 47 CG ARG A 125 20.666 −12.818 −7.108 1.00 9.42 ATOM 48 CD ARG A 125 20.699 −14.267 −7.204 1.00 9.93 ATOM 49 NE ARG A 125 19.498 −14.817 −7.811 1.00 10.17 ATOM 50 CZ ARG A 125 18.374 −15.094 −7.209 1.00 10.12 ATOM 51 NH1 ARG A 125 18.130 −14.785 −5.953 1.00 11.90 ATOM 52 NH2 ARG A 125 17.366 −15.651 −7.925 1.00 11.95 ATOM 53 C ARG A 125 21.904 −11.707 −10.604 1.00 7.86 ATOM 54 O ARG A 125 20.918 −11.954 −11.302 1.00 8.21 ATOM 55 N PHE A 126 22.827 −10.872 −11.028 1.00 8.60 ATOM 56 CA PHE A 126 22.801 −10.249 −12.361 1.00 8.84 ATOM 57 CB PHE A 126 24.050 −9.425 −12.607 1.00 9.25 ATOM 58 CG PHE A 126 24.065 −8.799 −13.961 1.00 9.36 ATOM 59 CD1 PHE A 126 23.304 −7.683 −14.306 1.00 9.98 ATOM 60 CE1 PHE A 126 23.363 −7.209 −15.599 1.00 11.58 ATOM 61 CZ PHE A 126 24.153 −7.848 −16.535 1.00 11.96 ATOM 62 CE2 PHE A 126 24.880 −8.914 −16.188 1.00 14.57 ATOM 63 CD2 PHE A 126 24.843 −9.393 −14.935 1.00 13.51 ATOM 64 C PHE A 126 22.612 −11.267 −13.439 1.00 9.49 ATOM 65 O PHE A 126 21.837 −11.052 −14.352 1.00 8.19 ATOM 66 N ASP A 127 23.307 −12.413 −13.390 1.00 9.86 ATOM 67 CA ASP A 127 23.174 −13.341 −14.446 1.00 10.89 ATOM 68 CB ASP A 127 24.174 −14.556 −14.268 1.00 15.11 ATOM 69 CG ASP A 127 25.579 −14.187 −14.653 1.00 22.69 ATOM 70 OD1 ASP A 127 25.873 −13.082 −15.210 1.00 22.53 ATOM 71 OD2 ASP A 127 26.444 −15.024 −14.308 1.00 26.82 ATOM 72 C ASP A 127 21.766 −13.924 −14.575 1.00 9.48 ATOM 73 O ASP A 127 21.349 −14.326 −15.672 1.00 11.55 ATOM 74 N ASP A 128 20.999 −13.981 −13.480 1.00 8.00 ATOM 75 CA ASP A 128 19.615 −14.385 −13.521 1.00 8.20 ATOM 76 CB ASP A 128 19.094 −14.733 −12.101 1.00 8.81 ATOM 77 CG ASP A 128 19.722 −15.981 −11.478 1.00 10.78 ATOM 78 OD1 ASP A 128 20.059 −16.855 −12.295 1.00 12.48 ATOM 79 OD2 ASP A 128 19.896 −15.999 −10.251 1.00 14.01 ATOM 80 C ASP A 128 18.689 −13.291 −14.160 1.00 7.71 ATOM 81 O ASP A 128 17.593 −13.630 −14.571 1.00 7.83 ATOM 82 N GLN A 129 19.199 −12.074 −14.240 1.00 6.43 ATOM 83 CA GLN A 129 18.416 −10.955 −14.789 1.00 6.39 ATOM 84 CB GLN A 129 18.811 −9.648 −14.078 1.00 6.11 ATOM 85 CG GLN A 129 18.425 −9.676 −12.616 1.00 6.68 ATOM 86 CD GLN A 129 18.695 −8.400 −11.926 1.00 6.03 ATOM 87 OE1 GLN A 129 19.840 −7.979 −11.719 1.00 6.44 ATOM 88 NE2 GLN A 129 17.604 −7.700 −11.561 1.00 6.15 ATOM 89 C GLN A 129 18.653 −10.892 −16.296 1.00 5.72 ATOM 90 O GLN A 129 19.160 −9.980 −16.866 1.00 6.33 ATOM 91 N TRP A 130 18.126 −11.965 −16.962 1.00 6.05 ATOM 92 CA TRP A 130 18.247 −12.126 −18.417 1.00 6.09 ATOM 93 CB TRP A 130 17.480 −13.366 −18.886 1.00 5.99 ATOM 94 CG TRP A 130 16.035 −13.384 −18.514 1.00 5.87 ATOM 95 CD1 TRP A 130 15.458 −13.937 −17.452 1.00 5.96 ATOM 96 NE1 TRP A 130 14.116 −13.655 −17.416 1.00 5.57 ATOM 97 CE2 TRP A 130 13.789 −12.902 −18.504 1.00 5.98 ATOM 98 CD2 TRP A 130 14.953 −12.717 −19.236 1.00 5.87 ATOM 99 CE3 TRP A 130 14.861 −12.000 −20.434 1.00 5.98 ATOM 100 CZ3 TRP A 130 13.643 −11.463 −20.799 1.00 6.46 ATOM 101 CH2 TRP A 130 12.511 −11.651 −20.066 1.00 6.18 ATOM 102 CZ2 TRP A 130 12.532 −12.356 −18.896 1.00 5.90 ATOM 103 C TRP A 130 17.767 −10.899 −19.186 1.00 5.42 ATOM 104 O TRP A 130 18.228 −10.555 −20.278 1.00 6.46 ATOM 105 N HIS A 131 16.741 −10.283 −18.564 1.00 4.95 ATOM 106 CA HIS A 131 16.069 −9.153 −19.107 1.00 5.15 ATOM 107 CB HIS A 131 14.772 −8.891 −18.342 1.00 5.21 ATOM 108 CG HIS A 131 14.906 −9.045 −16.875 1.00 4.87 ATOM 109 ND1 HIS A 131 15.205 −7.983 −16.052 1.00 4.65 ATOM 110 CE1 HIS A 131 15.363 −8.469 −14.813 1.00 4.50 ATOM 111 NE2 HIS A 131 15.151 −9.755 −14.766 1.00 5.16 ATOM 112 CD2 HIS A 131 14.872 −10.155 −16.055 1.00 5.36 ATOM 113 C HIS A 131 16.929 −7.890 −19.263 1.00 5.25 ATOM 114 O HIS A 131 16.562 −6.955 −19.943 1.00 5.30 ATOM 115 N TYR A 132 18.095 −7.854 −18.637 1.00 5.21 ATOM 116 CA TYR A 132 19.044 −6.791 −18.804 1.00 5.49 ATOM 117 CB TYR A 132 19.803 −6.562 −17.500 1.00 5.82 ATOM 118 CG TYR A 132 18.993 −6.055 −16.284 1.00 5.64 ATOM 119 CD1 TYR A 132 17.693 −5.548 −16.424 1.00 5.42 ATOM 120 CE1 TYR A 132 17.045 −5.089 −15.305 1.00 5.41 ATOM 121 CZ TYR A 132 17.649 −5.123 −14.072 1.00 5.62 ATOM 122 OH TYR A 132 16.948 −4.640 −12.949 1.00 5.68 ATOM 123 CE2 TYR A 132 18.910 −5.660 −13.936 1.00 6.31 ATOM 124 CD2 TYR A 132 19.563 −6.124 −15.033 1.00 6.18 ATOM 125 C TYR A 132 20.044 −6.965 −19.907 1.00 6.62 ATOM 126 O TYR A 132 20.582 −5.969 −20.367 1.00 7.65 ATOM 127 N TYR A 133 20.283 −8.213 −20.307 1.00 7.67 ATOM 128 CA TYR A 133 21.479 −8.416 −21.169 1.00 8.37 ATOM 129 CB TYR A 133 22.705 −8.809 −20.299 1.00 8.45 ATOM 130 CG TYR A 133 22.630 −10.151 −19.668 1.00 8.83 ATOM 131 CD1 TYR A 133 23.098 −11.270 −20.341 1.00 10.06 ATOM 132 CE1 TYR A 133 23.071 −12.473 −19.825 1.00 9.95 ATOM 133 CZ TYR A 133 22.501 −12.738 −18.554 1.00 9.62 ATOM 134 OH TYR A 133 22.403 −13.985 −18.058 1.00 10.83 ATOM 135 CE2 TYR A 133 22.009 −11.572 −17.816 1.00 8.72 ATOM 136 CD2 TYR A 133 22.115 −10.371 −18.399 1.00 8.80 ATOM 137 C TYR A 133 21.329 −9.511 −22.209 1.00 9.19 ATOM 138 O TYR A 133 22.199 −9.523 −23.145 1.00 9.96 ATOM 139 N GLU A 134 20.391 −10.422 −22.157 1.00 8.11 ATOM 140 CA GLU A 134 20.365 −11.521 −23.238 1.00 7.08 ATOM 141 CB GLU A 134 19.391 −12.595 −22.845 1.00 8.11 ATOM 142 CG GLU A 134 19.934 −13.532 −21.869 1.00 8.82 ATOM 143 CD GLU A 134 19.116 −14.781 −21.637 1.00 9.85 ATOM 144 OE1 GLU A 134 17.957 −14.861 −22.076 1.00 8.68 ATOM 145 OE2 GLU A 134 19.616 −15.714 −20.952 1.00 11.89 ATOM 146 C GLU A 134 19.953 −10.899 −24.550 1.00 8.65 ATOM 147 O GLU A 134 19.203 −9.935 −24.656 1.00 8.62 ATOM 148 N GLN A 135 20.450 −11.509 −25.671 1.00 9.63 ATOM 149 CA GLN A 135 20.189 −10.943 −26.968 1.00 10.15 ATOM 150 CB GLN A 135 21.054 −11.685 −28.032 1.00 12.60 ATOM 151 CG GLN A 135 22.542 −11.350 −27.805 1.00 18.13 ATOM 152 CD GLN A 135 22.879 −9.873 −27.878 1.00 26.31 ATOM 153 OE1 GLN A 135 23.520 −9.267 −26.969 1.00 26.28 ATOM 154 NE2 GLN A 135 22.435 −9.267 −28.962 1.00 27.49 ATOM 155 C GLN A 135 18.693 −10.990 −27.366 1.00 8.55 ATOM 156 O GLN A 135 18.198 −10.067 −27.967 1.00 10.28 ATOM 157 N ALA A 136 18.019 −12.059 −27.081 1.00 8.00 ATOM 158 CA ALA A 136 16.653 −12.189 −27.590 1.00 7.69 ATOM 159 CB ALA A 136 16.153 −13.621 −27.496 1.00 9.15 ATOM 160 C ALA A 136 15.701 −11.229 −26.878 1.00 7.63 ATOM 161 O ALA A 136 14.955 −10.490 −27.577 1.00 7.99 ATOM 162 N GLY A 137 15.668 −11.243 −25.574 1.00 7.71 ATOM 163 CA GLY A 137 14.631 −10.510 −24.800 1.00 7.92 ATOM 164 C GLY A 137 15.204 −9.425 −23.890 1.00 7.52 ATOM 165 O GLY A 137 14.391 −8.776 −23.200 1.00 8.16 ATOM 166 N GLY A 138 16.490 −9.148 −23.899 1.00 5.93 ATOM 167 CA GLY A 138 17.042 −8.195 −22.980 1.00 6.11 ATOM 168 C GLY A 138 17.106 −6.795 −23.466 1.00 6.67 ATOM 169 O GLY A 138 16.886 −6.459 −24.627 1.00 6.87 ATOM 170 N LEU A 139 17.411 −5.885 −22.510 1.00 6.36 ATOM 171 CA LEU A 139 17.382 −4.450 −22.709 1.00 6.71 ATOM 172 CB LEU A 139 16.980 −3.756 −21.396 1.00 6.50 ATOM 173 CG LEU A 139 16.164 −2.485 −21.503 1.00 6.37 ATOM 174 CD1 LEU A 139 14.747 −2.724 −21.977 1.00 7.38 ATOM 175 CD2 LEU A 139 16.209 −1.738 −20.179 1.00 7.55 ATOM 176 C LEU A 139 18.666 −3.828 −23.247 1.00 6.39 ATOM 177 O LEU A 139 18.747 −2.612 −23.425 1.00 6.92 ATOM 178 N ASN A 140 19.711 −4.644 −23.442 1.00 7.10 ATOM 179 CA ASN A 140 20.991 −4.183 −23.965 1.00 7.46 ATOM 180 CB ASN A 140 20.855 −3.698 −25.435 1.00 8.40 ATOM 181 CG ASN A 140 22.156 −3.735 −26.199 1.00 8.55 ATOM 182 OD1 ASN A 140 23.057 −4.554 −25.874 1.00 12.09 ATOM 183 ND2 ASN A 140 22.273 −2.863 −27.147 1.00 9.75 ATOM 184 C ASN A 140 21.621 −3.140 −23.075 1.00 7.14 ATOM 185 O ASN A 140 22.116 −2.130 −23.565 1.00 8.27 ATOM 186 N LEU A 141 21.622 −3.431 −21.771 1.00 6.74 ATOM 187 CA LEU A 141 22.118 −2.479 −20.756 1.00 6.84 ATOM 188 CB LEU A 141 21.472 −2.767 −19.455 1.00 6.87 ATOM 189 CG LEU A 141 20.085 −2.141 −19.336 1.00 6.24 ATOM 190 CD1 LEU A 141 19.255 −2.811 −18.259 1.00 6.64 ATOM 191 CD2 LEU A 141 20.188 −0.624 −19.106 1.00 6.68 ATOM 192 C LEU A 141 23.649 −2.416 −20.632 1.00 7.84 ATOM 193 O LEU A 141 24.143 −1.318 −20.401 1.00 8.32 ATOM 194 N PRO A 142 24.408 −3.537 −20.688 1.00 7.52 ATOM 195 CA PRO A 142 25.845 −3.365 −20.360 1.00 7.97 ATOM 196 CB PRO A 142 26.378 −4.793 −20.623 1.00 9.69 ATOM 197 CG PRO A 142 25.239 −5.692 −20.199 1.00 9.00 ATOM 198 CD PRO A 142 24.013 −4.948 −20.773 1.00 8.29 ATOM 199 C PRO A 142 26.525 −2.304 −21.176 1.00 8.88 ATOM 200 O PRO A 142 27.301 −1.479 −20.549 1.00 9.95 ATOM 201 N THR A 143 26.301 −2.181 −22.438 1.00 9.83 ATOM 202 CA THR A 143 26.899 −1.154 −23.210 1.00 10.77 ATOM 203 CB THR A 143 26.687 −1.432 −24.765 1.00 14.94 ATOM 204 OG1 THR A 143 27.409 −2.654 −24.983 1.00 21.55 ATOM 205 CG2 THR A 143 27.216 −0.226 −25.618 1.00 16.90 ATOM 206 C THR A 143 26.403 0.221 −22.865 1.00 10.24 ATOM 207 O THR A 143 27.100 1.234 −22.849 1.00 10.72 ATOM 208 N ALA A 144 25.073 0.324 −22.537 1.00 8.50 ATOM 209 CA ALA A 144 24.550 1.598 −22.122 1.00 8.05 ATOM 210 CB ALA A 144 23.035 1.470 −21.898 1.00 7.44 ATOM 211 C ALA A 144 25.237 2.144 −20.866 1.00 7.32 ATOM 212 O ALA A 144 25.521 3.361 −20.786 1.00 7.84 ATOM 213 N TRP A 145 25.455 1.256 −19.926 1.00 7.73 ATOM 214 CA TRP A 145 26.081 1.627 −18.629 1.00 7.48 ATOM 215 CB TRP A 145 26.021 0.462 −17.729 1.00 6.84 ATOM 216 CG TRP A 145 24.613 0.115 −17.183 1.00 6.67 ATOM 217 CD1 TRP A 145 23.595 0.983 −16.893 1.00 6.81 ATOM 218 NE1 TRP A 145 22.583 0.308 −16.369 1.00 6.25 ATOM 219 CE2 TRP A 145 22.856 −1.019 −16.269 1.00 6.87 ATOM 220 CD2 TRP A 145 24.178 −1.164 −16.783 1.00 6.25 ATOM 221 CE3 TRP A 145 24.731 −2.467 −16.817 1.00 7.18 ATOM 222 CZ3 TRP A 145 24.046 −3.500 −16.388 1.00 8.07 ATOM 223 CH2 TRP A 145 22.690 −3.309 −15.862 1.00 9.20 ATOM 224 CZ2 TRP A 145 22.167 −2.079 −15.751 1.00 7.47 ATOM 225 C TRP A 145 27.517 2.122 −18.805 1.00 8.30 ATOM 226 O TRP A 145 27.986 2.809 −17.866 1.00 9.06 ATOM 227 N ASP A 146 28.195 1.887 −19.921 1.00 9.00 ATOM 228 CA ASP A 146 29.496 2.522 −20.187 1.00 9.41 ATOM 229 CB ASP A 146 30.070 1.930 −21.484 1.00 9.73 ATOM 230 CG ASP A 146 30.506 0.542 −21.315 1.00 11.08 ATOM 231 OD1 ASP A 146 30.815 0.077 −20.211 1.00 13.32 ATOM 232 OD2 ASP A 146 30.435 −0.198 −22.373 1.00 15.55 ATOM 233 C ASP A 146 29.360 4.003 −20.245 1.00 12.39 ATOM 234 O ASP A 146 30.387 4.736 −20.091 1.00 13.71 ATOM 235 N THR A 147 28.180 4.585 −20.570 1.00 11.95 ATOM 236 CA THR A 147 27.963 6.012 −20.807 1.00 13.21 ATOM 237 CB THR A 147 27.273 6.162 −22.303 1.00 12.96 ATOM 238 OG1 THR A 147 28.267 5.662 −23.258 1.00 24.83 ATOM 239 CG2 THR A 147 27.031 7.563 −22.606 1.00 21.42 ATOM 240 C THR A 147 27.015 6.650 −19.796 1.00 10.15 ATOM 241 O THR A 147 27.076 7.814 −19.455 1.00 10.91 ATOM 242 N ALA A 148 25.948 5.897 −19.361 1.00 8.46 ATOM 243 CA ALA A 148 24.923 6.494 −18.501 1.00 8.41 ATOM 244 CB ALA A 148 23.760 7.022 −19.329 1.00 9.12 ATOM 245 C ALA A 148 24.422 5.417 −17.548 1.00 7.39 ATOM 246 O ALA A 148 24.166 4.293 −17.935 1.00 7.98 ATOM 247 N THR A 149 24.113 5.880 −16.348 1.00 7.48 ATOM 248 CA THR A 149 23.594 5.028 −15.231 1.00 7.88 ATOM 249 CB THR A 149 24.690 4.681 −14.254 1.00 8.71 ATOM 250 OG1 THR A 149 25.259 5.926 −13.759 1.00 9.67 ATOM 251 CG2 THR A 149 25.759 3.827 −14.939 1.00 9.70 ATOM 252 C THR A 149 22.418 5.693 −14.478 1.00 6.60 ATOM 253 O THR A 149 21.920 5.103 −13.521 1.00 7.10 ATOM 254 N GLY A 150 22.030 6.873 −14.891 1.00 7.48 ATOM 255 CA GLY A 150 20.979 7.631 −14.186 1.00 7.06 ATOM 256 C GLY A 150 21.390 8.566 −13.157 1.00 7.91 ATOM 257 O GLY A 150 20.572 9.141 −12.468 1.00 7.26 ATOM 258 N SER A 151 22.723 8.812 −13.050 1.00 8.63 ATOM 259 CA SER A 151 23.218 9.771 −12.004 1.00 8.89 ATOM 260 CB SER A 151 24.681 10.023 −12.257 1.00 12.29 ATOM 261 OG SER A 151 25.413 8.877 −12.137 1.00 18.84 ATOM 262 C SER A 151 22.547 11.127 −12.114 1.00 8.29 ATOM 263 O SER A 151 22.452 11.690 −13.199 1.00 9.10 ATOM 264 N GLY A 152 21.990 11.609 −10.979 1.00 9.13 ATOM 265 CA GLY A 152 21.363 12.902 −10.954 1.00 9.18 ATOM 266 C GLY A 152 19.882 12.926 −11.330 1.00 10.01 ATOM 267 O GLY A 152 19.272 14.021 −11.318 1.00 13.79 ATOM 268 N VAL A 153 19.349 11.792 −11.704 1.00 7.27 ATOM 269 CA VAL A 153 17.959 11.716 −12.202 1.00 6.62 ATOM 270 CB VAL A 153 17.957 10.865 −13.499 1.00 6.53 ATOM 271 CG1 VAL A 153 16.564 10.809 −14.055 1.00 6.48 ATOM 272 CG2 VAL A 153 18.898 11.454 −14.525 1.00 7.05 ATOM 273 C VAL A 153 17.081 11.105 −11.134 1.00 5.94 ATOM 274 O VAL A 153 17.521 10.270 −10.339 1.00 6.77 ATOM 275 N VAL A 154 15.818 11.578 −11.092 1.00 6.25 ATOM 276 CA VAL A 154 14.821 11.139 −10.143 1.00 5.63 ATOM 277 CB VAL A 154 14.333 12.307 −9.267 1.00 6.01 ATOM 278 CG1 VAL A 154 13.200 11.879 −8.345 1.00 6.81 ATOM 279 CG2 VAL A 154 15.484 12.865 −8.462 1.00 7.80 ATOM 280 C VAL A 154 13.658 10.463 −10.874 1.00 5.23 ATOM 281 O VAL A 154 13.100 11.060 −11.821 1.00 6.11 ATOM 282 N VAL A 155 13.339 9.260 −10.425 1.00 5.35 ATOM 283 CA VAL A 155 12.200 8.499 −10.938 1.00 4.93 ATOM 284 CB VAL A 155 12.638 7.114 −11.529 1.00 4.95 ATOM 285 CG1 VAL A 155 11.422 6.374 −12.101 1.00 5.12 ATOM 286 CG2 VAL A 155 13.742 7.265 −12.562 1.00 5.75 ATOM 287 C VAL A 155 11.196 8.336 −9.878 1.00 4.39 ATOM 288 O VAL A 155 11.490 7.763 −8.814 1.00 5.65 ATOM 289 N ALA A 156 9.953 8.791 −10.097 1.00 4.41 ATOM 290 CA ALA A 156 8.840 8.527 −9.147 1.00 4.84 ATOM 291 CB ALA A 156 7.903 9.733 −9.116 1.00 5.28 ATOM 292 C ALA A 156 8.167 7.230 −9.548 1.00 4.82 ATOM 293 O ALA A 156 7.791 7.055 −10.697 1.00 6.06 ATOM 294 N VAL A 157 8.000 6.341 −8.591 1.00 4.18 ATOM 295 CA VAL A 157 7.340 5.029 −8.780 1.00 4.79 ATOM 296 CB VAL A 157 8.208 3.888 −8.288 1.00 5.10 ATOM 297 CG1 VAL A 157 7.441 2.614 −8.261 1.00 5.83 ATOM 298 CG2 VAL A 157 9.456 3.788 −9.128 1.00 5.17 ATOM 299 C VAL A 157 6.017 5.108 −8.084 1.00 4.51 ATOM 300 O VAL A 157 5.972 5.181 −6.840 1.00 4.98 ATOM 301 N LEU A 158 4.918 5.155 −8.848 1.00 4.40 ATOM 302 CA LEU A 158 3.559 5.297 −8.329 1.00 4.68 ATOM 303 CB LEU A 158 2.714 6.194 −9.247 1.00 5.68 ATOM 304 CG LEU A 158 2.828 7.695 −9.027 1.00 6.39 ATOM 305 CD1 LEU A 158 4.251 8.181 −9.264 1.00 6.73 ATOM 306 CD2 LEU A 158 1.889 8.435 −9.973 1.00 7.00 ATOM 307 C LEU A 158 2.989 3.925 −8.212 1.00 4.90 ATOM 308 O LEU A 158 2.713 3.294 −9.249 1.00 5.24 ATOM 309 N ASP A 159 2.855 3.386 −7.008 1.00 5.11 ATOM 310 CA ASP A 159 2.662 1.949 −6.851 1.00 5.57 ATOM 311 CB ASP A 159 3.981 1.213 −7.238 1.00 5.88 ATOM 312 CG ASP A 159 3.761 −0.070 −7.839 1.00 7.36 ATOM 313 OD1 ASP A 159 3.235 −0.982 −7.184 1.00 7.67 ATOM 314 OD2 ASP A 159 4.146 −0.313 −8.995 1.00 8.72 ATOM 315 C ASP A 159 2.136 1.689 −5.475 1.00 5.20 ATOM 316 O ASP A 159 1.513 2.554 −4.799 1.00 5.18 ATOM 317 N THR A 160 2.429 0.477 −4.959 1.00 4.95 ATOM 318 CA THR A 160 1.925 0.033 −3.650 1.00 5.28 ATOM 319 CB THR A 160 1.822 −1.517 −3.571 1.00 5.73 ATOM 320 OG1 THR A 160 3.187 −2.060 −3.602 1.00 6.05 ATOM 321 CG2 THR A 160 1.042 −2.101 −4.719 1.00 6.49 ATOM 322 C THR A 160 2.739 0.549 −2.473 1.00 5.53 ATOM 323 O THR A 160 2.405 0.243 −1.335 1.00 6.23 ATOM 324 N GLY A 161 3.789 1.313 −2.745 1.00 5.28 ATOM 325 CA GLY A 161 4.774 1.664 −1.762 1.00 5.79 ATOM 326 C GLY A 161 5.983 0.811 −1.821 1.00 5.26 ATOM 327 O GLY A 161 6.169 0.052 −2.800 1.00 6.39 ATOM 328 N TYR A 162 6.844 0.848 −0.827 1.00 5.56 ATOM 329 CA TYR A 162 8.097 0.075 −0.835 1.00 5.56 ATOM 330 CB TYR A 162 9.265 0.926 −1.314 1.00 6.51 ATOM 331 CG TYR A 162 9.733 1.923 −0.293 1.00 6.58 ATOM 332 CD1 TYR A 162 9.012 3.044 0.058 1.00 6.65 ATOM 333 CE1 TYR A 162 9.405 3.876 1.035 1.00 7.04 ATOM 334 CZ TYR A 162 10.553 3.647 1.718 1.00 7.73 ATOM 335 OH TYR A 162 10.955 4.541 2.705 1.00 10.14 ATOM 336 CE2 TYR A 162 11.315 2.597 1.409 1.00 7.11 ATOM 337 CD2 TYR A 162 10.919 1.754 0.418 1.00 7.03 ATOM 338 C TYR A 162 8.391 −0.502 0.557 1.00 5.64 ATOM 339 O TYR A 162 7.876 0.032 1.557 1.00 5.57 ATOM 340 N ARG A 163 9.228 −1.532 0.581 1.00 5.90 ATOM 341 CA ARG A 163 9.928 −1.887 1.825 1.00 7.08 ATOM 342 CB ARG A 163 9.820 −3.355 2.128 1.00 7.63 ATOM 343 CG ARG A 163 8.436 −3.879 2.345 1.00 7.54 ATOM 344 CD ARG A 163 7.603 −3.123 3.314 1.00 7.64 ATOM 345 NE ARG A 163 8.278 −2.861 4.582 1.00 6.89 ATOM 346 CZ ARG A 163 7.794 −2.034 5.522 1.00 7.32 ATOM 347 NH1 ARG A 163 8.544 −1.769 6.574 1.00 7.85 ATOM 348 NH2 ARG A 163 6.649 −1.487 5.354 1.00 7.79 ATOM 349 C ARG A 163 11.367 −1.478 1.684 1.00 7.26 ATOM 350 O ARG A 163 11.938 −1.468 0.556 1.00 6.77 ATOM 351 N PRO A 164 12.034 −1.113 2.782 1.00 7.12 ATOM 352 CA PRO A 164 13.416 −0.633 2.751 1.00 7.02 ATOM 353 CB PRO A 164 13.555 0.093 4.102 1.00 7.65 ATOM 354 CG PRO A 164 12.750 −0.758 4.978 1.00 7.06 ATOM 355 CD PRO A 164 11.498 −1.079 4.178 1.00 6.95 ATOM 356 C PRO A 164 14.427 −1.764 2.598 1.00 7.37 ATOM 357 O PRO A 164 15.363 −1.954 3.411 1.00 7.96 ATOM 358 N HIS A 165 14.290 −2.481 1.522 1.00 6.81 ATOM 359 CA HIS A 165 15.125 −3.673 1.223 1.00 6.19 ATOM 360 CB HIS A 165 14.679 −4.200 −0.176 1.00 6.54 ATOM 361 CG HIS A 165 15.271 −5.517 −0.551 1.00 6.60 ATOM 362 ND1 HIS A 165 16.618 −5.748 −0.705 1.00 6.75 ATOM 363 CE1 HIS A 165 16.771 −7.001 −1.106 1.00 6.97 ATOM 364 NE2 HIS A 165 15.569 −7.536 −1.252 1.00 7.18 ATOM 365 CD2 HIS A 165 14.625 −6.634 −0.908 1.00 6.70 ATOM 366 C HIS A 165 16.567 −3.227 1.250 1.00 6.19 ATOM 367 O HIS A 165 16.940 −2.172 0.713 1.00 6.52 ATOM 368 N ALA A 166 17.412 −4.085 1.794 1.00 6.05 ATOM 369 CA ALA A 166 18.841 −3.807 1.833 1.00 6.22 ATOM 370 CB ALA A 166 19.614 −4.979 2.497 1.00 7.60 ATOM 371 C ALA A 166 19.509 −3.517 0.535 1.00 6.60 ATOM 372 O ALA A 166 20.508 −2.802 0.426 1.00 7.57 ATOM 373 N ASP A 167 18.901 −4.071 −0.574 1.00 6.35 ATOM 374 CA ASP A 167 19.455 −3.860 −1.928 1.00 6.24 ATOM 375 CB ASP A 167 19.486 −5.159 −2.748 1.00 6.79 ATOM 376 CG ASP A 167 20.595 −5.255 −3.726 1.00 7.36 ATOM 377 OD1 ASP A 167 21.695 −4.689 −3.476 1.00 7.55 ATOM 378 OD2 ASP A 167 20.397 −5.910 −4.814 1.00 6.66 ATOM 379 C ASP A 167 18.764 −2.761 −2.723 1.00 6.29 ATOM 380 O ASP A 167 18.869 −2.673 −3.912 1.00 6.98 ATOM 381 N LEU A 168 17.996 −1.941 −1.964 1.00 6.39 ATOM 382 CA LEU A 168 17.204 −0.839 −2.585 1.00 6.26 ATOM 383 CB LEU A 168 15.713 −1.293 −2.633 1.00 7.18 ATOM 384 CG LEU A 168 14.749 −0.313 −3.306 1.00 8.10 ATOM 385 CD1 LEU A 168 15.153 0.006 −4.742 1.00 9.20 ATOM 386 CD2 LEU A 168 13.363 −0.861 −3.191 1.00 9.23 ATOM 387 C LEU A 168 17.343 0.471 −1.818 1.00 6.50 ATOM 388 O LEU A 168 17.319 1.541 −2.420 1.00 6.32 ATOM 389 N ASN A 169 17.295 0.404 −0.483 1.00 6.84 ATOM 390 CA ASN A 169 16.980 1.614 0.302 1.00 7.08 ATOM 391 CB ASN A 169 16.843 1.239 1.785 1.00 8.36 ATOM 392 CG ASN A 169 16.147 2.245 2.526 1.00 10.04 ATOM 393 OD1 ASN A 169 16.751 2.855 3.514 1.00 16.72 ATOM 394 ND2 ASN A 169 14.976 2.518 2.202 1.00 9.54 ATOM 395 C ASN A 169 17.940 2.752 0.095 1.00 7.21 ATOM 396 O ASN A 169 17.519 3.903 0.194 1.00 7.74 ATOM 397 N ALA A 170 19.192 2.492 −0.164 1.00 6.89 ATOM 398 CA ALA A 170 20.094 3.618 −0.395 1.00 8.20 ATOM 399 CB ALA A 170 21.530 3.198 −0.592 1.00 9.33 ATOM 400 C ALA A 170 19.713 4.518 −1.538 1.00 8.07 ATOM 401 O ALA A 170 20.153 5.646 −1.671 1.00 9.94 ATOM 402 N ASN A 171 18.914 3.932 −2.526 1.00 6.67 ATOM 403 CA ASN A 171 18.440 4.703 −3.667 1.00 6.60 ATOM 404 CB ASN A 171 18.581 3.863 −4.942 1.00 6.96 ATOM 405 CG ASN A 171 19.974 3.734 −5.420 1.00 8.99 ATOM 406 OD1 ASN A 171 20.875 4.433 −4.889 1.00 10.77 ATOM 407 ND2 ASN A 171 20.206 2.852 −6.354 1.00 9.61 ATOM 408 C ASN A 171 17.060 5.200 −3.518 1.00 6.97 ATOM 409 O ASN A 171 16.472 5.735 −4.503 1.00 7.86 ATOM 410 N ILE A 172 16.454 5.153 −2.355 1.00 6.72 ATOM 411 CA ILE A 172 15.100 5.676 −2.059 1.00 7.31 ATOM 412 CB ILE A 172 14.353 4.688 −1.150 1.00 7.92 ATOM 413 CG1 ILE A 172 14.097 3.383 −1.802 1.00 8.61 ATOM 414 CD1 ILE A 172 13.079 3.404 −2.877 1.00 12.01 ATOM 415 CG2 ILE A 172 13.083 5.316 −0.552 1.00 9.61 ATOM 416 C ILE A 172 15.181 7.056 −1.448 1.00 8.27 ATOM 417 O ILE A 172 15.975 7.253 −0.495 1.00 11.02 ATOM 418 N LEU A 173 14.429 7.979 −1.935 1.00 7.10 ATOM 419 CA LEU A 173 14.229 9.322 −1.412 1.00 7.53 ATOM 420 CB LEU A 173 14.191 10.326 −2.555 1.00 8.24 ATOM 421 CG LEU A 173 15.519 10.597 −3.225 1.00 9.19 ATOM 422 CD1 LEU A 173 15.282 11.380 −4.501 1.00 10.78 ATOM 423 CD2 LEU A 173 16.407 11.388 −2.226 1.00 11.16 ATOM 424 C LEU A 173 12.932 9.370 −0.651 1.00 7.76 ATOM 425 O LEU A 173 12.056 8.522 −0.801 1.00 7.69 ATOM 426 N PRO A 174 12.694 10.390 0.186 1.00 7.85 ATOM 427 CA PRO A 174 11.417 10.584 0.764 1.00 7.82 ATOM 428 CB PRO A 174 11.532 11.980 1.473 1.00 9.30 ATOM 429 CG PRO A 174 12.984 12.061 1.802 1.00 10.51 ATOM 430 CD PRO A 174 13.661 11.416 0.611 1.00 8.84 ATOM 431 C PRO A 174 10.333 10.696 −0.307 1.00 7.30 ATOM 432 O PRO A 174 10.553 11.260 −1.381 1.00 7.76 ATOM 433 N GLY A 175 9.154 10.200 0.001 1.00 6.79 ATOM 434 CA GLY A 175 7.996 10.210 −0.914 1.00 6.26 ATOM 435 C GLY A 175 6.765 10.639 −0.273 1.00 5.54 ATOM 436 O GLY A 175 6.762 11.489 0.638 1.00 7.38 ATOM 437 N TYR A 176 5.644 10.116 −0.721 1.00 5.71 ATOM 438 CA TYR A 176 4.359 10.473 −0.119 1.00 5.79 ATOM 439 CB TYR A 176 3.806 11.798 −0.712 1.00 6.83 ATOM 440 CG TYR A 176 3.016 12.598 0.351 1.00 7.97 ATOM 441 CD1 TYR A 176 3.687 13.341 1.283 1.00 8.82 ATOM 442 CE1 TYR A 176 3.066 13.964 2.339 1.00 10.20 ATOM 443 CZ TYR A 176 1.819 13.810 2.453 1.00 10.76 ATOM 444 OH TYR A 176 1.202 14.472 3.554 1.00 18.66 ATOM 445 CE2 TYR A 176 1.005 13.174 1.617 1.00 10.48 ATOM 446 CD2 TYR A 176 1.627 12.474 0.495 1.00 9.61 ATOM 447 C TYR A 176 3.338 9.404 −0.355 1.00 5.80 ATOM 448 O TYR A 176 3.436 8.664 −1.367 1.00 6.30 ATOM 449 N ASP A 177 2.389 9.249 0.547 1.00 5.45 ATOM 450 CA ASP A 177 1.336 8.285 0.504 1.00 5.69 ATOM 451 CB ASP A 177 1.344 7.460 1.813 1.00 5.95 ATOM 452 CG ASP A 177 0.375 6.348 1.824 1.00 5.42 ATOM 453 OD1 ASP A 177 −0.630 6.289 1.123 1.00 6.78 ATOM 454 OD2 ASP A 177 0.612 5.414 2.691 1.00 6.08 ATOM 455 C ASP A 177 0.048 8.992 0.265 1.00 6.83 ATOM 456 O ASP A 177 −0.480 9.725 1.129 1.00 6.64 ATOM 457 N MET A 178 −0.490 8.846 −0.959 1.00 6.59 ATOM 458 CA MET A 178 −1.678 9.512 −1.408 1.00 7.10 ATOM 459 CB MET A 178 −1.605 9.706 −2.908 1.00 8.66 ATOM 460 CG MET A 178 −0.513 10.596 −3.376 1.00 12.57 ATOM 461 SD MET A 178 −0.880 12.356 −2.735 1.00 14.68 ATOM 462 CE MET A 178 0.703 13.091 −3.292 1.00 16.71 ATOM 463 C MET A 178 −2.923 8.804 −1.129 1.00 7.38 ATOM 464 O MET A 178 −4.036 9.257 −1.524 1.00 7.66 ATOM 465 N ILE A 179 −2.905 7.638 −0.488 1.00 6.89 ATOM 466 CA ILE A 179 −4.128 6.845 −0.312 1.00 6.94 ATOM 467 CB ILE A 179 −3.868 5.388 0.004 1.00 6.92 ATOM 468 CG1 ILE A 179 −3.000 4.760 −1.050 1.00 7.39 ATOM 469 CD1 ILE A 179 −2.557 3.390 −0.662 1.00 7.15 ATOM 470 CG2 ILE A 179 −5.221 4.677 0.138 1.00 6.97 ATOM 471 C ILE A 179 −5.058 7.480 0.768 1.00 8.71 ATOM 472 O ILE A 179 −4.673 7.648 1.923 1.00 8.31 ATOM 473 N SER A 180 −6.237 7.910 0.285 1.00 8.81 ATOM 474 CA SER A 180 −7.198 8.630 1.181 1.00 10.10 ATOM 475 CB SER A 180 −8.028 9.621 0.281 1.00 12.44 ATOM 476 OG SER A 180 −7.273 10.522 −0.445 1.00 15.19 ATOM 477 C SER A 180 −8.111 7.675 1.930 1.00 11.84 ATOM 478 O SER A 180 −8.470 7.985 3.113 1.00 15.18 ATOM 479 N ASN A 181 −8.479 6.536 1.365 1.00 10.16 ATOM 480 CA ASN A 181 −9.420 5.595 2.008 1.00 10.79 ATOM 481 CB ASN A 181 −10.070 4.827 0.890 1.00 13.53 ATOM 482 CG ASN A 181 −11.030 3.766 1.345 1.00 17.13 ATOM 483 OD1 ASN A 181 −12.160 3.727 0.835 1.00 27.54 ATOM 484 ND2 ASN A 181 −10.644 2.874 2.148 1.00 15.26 ATOM 485 C ASN A 181 −8.698 4.766 3.032 1.00 12.42 ATOM 486 O ASN A 181 −7.657 4.115 2.672 1.00 10.80 ATOM 487 N LEU A 182 −9.080 4.817 4.282 1.00 12.94 ATOM 488 CA LEU A 182 −8.359 4.163 5.341 1.00 13.82 ATOM 489 CB LEU A 182 −9.001 4.520 6.725 1.00 17.63 ATOM 490 CG LEU A 182 −8.244 5.772 7.282 1.00 21.27 ATOM 491 CD1 LEU A 182 −8.515 7.063 6.546 1.00 22.77 ATOM 492 CD2 LEU A 182 −8.530 6.004 8.771 1.00 25.97 ATOM 493 C LEU A 182 −8.309 2.666 5.160 1.00 12.47 ATOM 494 O LEU A 182 −7.285 2.066 5.509 1.00 11.86 ATOM 495 N SER A 183 −9.370 2.062 4.649 1.00 13.51 ATOM 496 CA SER A 183 −9.408 0.626 4.481 1.00 14.42 ATOM 497 CB SER A 183 −10.801 0.147 4.016 1.00 18.23 ATOM 498 OG SER A 183 −11.693 0.448 5.055 1.00 27.95 ATOM 499 C SER A 183 −8.358 0.210 3.421 1.00 14.48 ATOM 500 O SER A 183 −7.677 −0.816 3.541 1.00 14.34 ATOM 501 N VAL A 184 −8.254 0.962 2.340 1.00 10.65 ATOM 502 CA VAL A 184 −7.299 0.682 1.274 1.00 9.80 ATOM 503 CB VAL A 184 −7.563 1.548 0.050 1.00 9.12 ATOM 504 CG1 VAL A 184 −6.513 1.370 −0.987 1.00 9.92 ATOM 505 CG2 VAL A 184 −8.959 1.230 −0.529 1.00 10.32 ATOM 506 C VAL A 184 −5.892 0.959 1.838 1.00 9.37 ATOM 507 O VAL A 184 −4.947 0.163 1.614 1.00 8.64 ATOM 508 N ALA A 185 −5.717 2.035 2.560 1.00 7.74 ATOM 509 CA ALA A 185 −4.409 2.496 3.014 1.00 8.40 ATOM 510 CB ALA A 185 −4.518 3.782 3.769 1.00 8.61 ATOM 511 C ALA A 185 −3.765 1.462 3.951 1.00 7.36 ATOM 512 O ALA A 185 −2.561 1.236 3.898 1.00 8.04 ATOM 513 N ASN A 186 −4.512 0.866 4.888 1.00 7.38 ATOM 514 CA ASN A 186 −4.053 −0.202 5.727 1.00 7.56 ATOM 515 CB ASN A 186 −3.698 −1.446 4.911 1.00 8.71 ATOM 516 CG ASN A 186 −3.419 −2.663 5.748 1.00 9.43 ATOM 517 OD1 ASN A 186 −4.042 −2.869 6.806 1.00 11.20 ATOM 518 ND2 ASN A 186 −2.466 −3.475 5.363 1.00 9.33 ATOM 519 C ASN A 186 −2.870 0.273 6.514 1.00 7.70 ATOM 520 O ASN A 186 −1.983 −0.556 6.813 1.00 8.52 ATOM 521 N ASP A 187 −2.829 1.517 6.960 1.00 7.97 ATOM 522 CA ASP A 187 −1.704 2.049 7.738 1.00 8.33 ATOM 523 CB ASP A 187 −0.717 2.777 6.809 1.00 7.56 ATOM 524 CG ASP A 187 −1.272 4.015 6.197 1.00 8.27 ATOM 525 OD1 ASP A 187 −2.468 4.348 6.451 1.00 7.87 ATOM 526 OD2 ASP A 187 −0.487 4.741 5.479 1.00 7.37 ATOM 527 C ASP A 187 −2.160 2.986 8.860 1.00 8.94 ATOM 528 O ASP A 187 −1.344 3.636 9.461 1.00 11.36 ATOM 529 N GLY A 188 −3.443 3.017 9.114 1.00 10.87 ATOM 530 CA GLY A 188 −3.977 3.736 10.284 1.00 12.33 ATOM 531 C GLY A 188 −4.503 5.108 9.968 1.00 13.89 ATOM 532 O GLY A 188 −5.087 5.774 10.892 1.00 15.82 ATOM 533 N GLY A 189 −4.443 5.647 8.757 1.00 10.60 ATOM 534 CA GLY A 189 −4.950 6.944 8.448 1.00 11.77 ATOM 535 C GLY A 189 −4.862 7.217 6.985 1.00 9.51 ATOM 536 O GLY A 189 −4.467 6.390 6.148 1.00 8.77 ATOM 537 N GLY A 190 −5.336 8.378 6.634 1.00 9.97 ATOM 538 CA GLY A 190 −5.334 8.901 5.280 1.00 9.16 ATOM 539 C GLY A 190 −4.024 9.320 4.775 1.00 8.98 ATOM 540 O GLY A 190 −2.971 8.745 5.127 1.00 8.01 ATOM 541 N ARG A 191 −3.972 10.327 3.952 1.00 7.94 ATOM 542 CA ARG A 191 −2.731 10.738 3.314 1.00 8.02 ATOM 543 CB ARG A 191 −2.963 11.871 2.266 1.00 8.51 ATOM 544 CG ARG A 191 −3.877 11.432 1.136 1.00 7.74 ATOM 545 CD ARG A 191 −3.902 12.559 0.083 1.00 8.51 ATOM 546 NE ARG A 191 −4.599 12.102 −1.104 1.00 8.28 ATOM 547 CZ ARG A 191 −4.911 12.871 −2.147 1.00 8.35 ATOM 548 NH1 ARG A 191 −4.629 14.149 −2.127 1.00 9.98 ATOM 549 NH2 ARG A 191 −5.409 12.285 −3.169 1.00 9.09 ATOM 550 C ARG A 191 −1.657 11.008 4.308 1.00 8.04 ATOM 551 O ARG A 191 −1.896 11.619 5.349 1.00 9.64 ATOM 552 N ASP A 192 −0.430 10.636 4.004 1.00 7.25 ATOM 553 CA ASP A 192 0.640 10.768 4.962 1.00 6.35 ATOM 554 CB ASP A 192 0.574 9.701 6.044 1.00 6.88 ATOM 555 CG ASP A 192 0.876 8.291 5.513 1.00 6.77 ATOM 556 OD1 ASP A 192 2.096 7.958 5.536 1.00 8.59 ATOM 557 OD2 ASP A 192 −0.003 7.556 5.101 1.00 6.72 ATOM 558 C ASP A 192 1.979 10.728 4.306 1.00 7.55 ATOM 559 O ASP A 192 2.092 10.273 3.133 1.00 7.78 ATOM 560 N SER A 193 3.053 11.101 4.960 1.00 6.92 ATOM 561 CA SER A 193 4.348 11.212 4.348 1.00 7.50 ATOM 562 CB SER A 193 5.240 12.283 4.984 1.00 9.40 ATOM 563 OG SER A 193 5.563 11.849 6.268 1.00 13.98 ATOM 564 C SER A 193 5.146 9.884 4.222 1.00 8.09 ATOM 565 O SER A 193 6.210 9.953 3.632 1.00 10.63 ATOM 566 N ASP A 194 4.610 8.792 4.779 1.00 6.40 ATOM 567 CA ASP A 194 5.391 7.522 4.747 1.00 6.88 ATOM 568 CB ASP A 194 5.285 6.892 6.135 1.00 7.25 ATOM 569 CG ASP A 194 6.120 5.683 6.255 1.00 10.11 ATOM 570 OD1 ASP A 194 6.667 5.164 5.277 1.00 8.47 ATOM 571 OD2 ASP A 194 6.331 5.187 7.397 1.00 10.52 ATOM 572 C ASP A 194 4.790 6.635 3.662 1.00 6.07 ATOM 573 O ASP A 194 3.717 6.058 3.799 1.00 6.06 ATOM 574 N ALA A 195 5.547 6.457 2.575 1.00 5.69 ATOM 575 CA ALA A 195 5.146 5.690 1.413 1.00 5.95 ATOM 576 CB ALA A 195 5.736 6.331 0.154 1.00 6.97 ATOM 577 C ALA A 195 5.528 4.270 1.505 1.00 5.77 ATOM 578 O ALA A 195 5.492 3.503 0.525 1.00 6.21 ATOM 579 N ARG A 196 5.943 3.757 2.686 1.00 5.76 ATOM 580 CA ARG A 196 6.167 2.338 2.814 1.00 5.39 ATOM 581 CB ARG A 196 6.839 2.012 4.156 1.00 5.31 ATOM 582 CG ARG A 196 8.262 2.402 4.193 1.00 5.59 ATOM 583 CD ARG A 196 8.965 2.196 5.509 1.00 6.11 ATOM 584 NE ARG A 196 10.318 2.671 5.486 1.00 5.99 ATOM 585 CZ ARG A 196 11.291 2.307 6.362 1.00 6.28 ATOM 586 NH1 ARG A 196 11.064 1.394 7.261 1.00 6.70 ATOM 587 NH2 ARG A 196 12.480 2.848 6.217 1.00 6.61 ATOM 588 C ARG A 196 4.923 1.534 2.624 1.00 5.46 ATOM 589 O ARG A 196 3.805 1.989 2.885 1.00 6.75 ATOM 590 N ASP A 197 5.087 0.312 2.118 1.00 5.18 ATOM 591 CA ASP A 197 4.000 −0.590 1.838 1.00 5.46 ATOM 592 CB ASP A 197 4.408 −1.513 0.700 1.00 5.77 ATOM 593 CG ASP A 197 3.339 −2.365 0.133 1.00 5.07 ATOM 594 OD1 ASP A 197 2.233 −2.457 0.741 1.00 5.88 ATOM 595 OD2 ASP A 197 3.582 −3.024 −0.908 1.00 6.33 ATOM 596 C ASP A 197 3.705 −1.415 3.064 1.00 5.80 ATOM 597 O ASP A 197 4.553 −2.239 3.480 1.00 6.13 ATOM 598 N PRO A 198 2.518 −1.280 3.680 1.00 5.31 ATOM 599 CA PRO A 198 2.163 −2.136 4.851 1.00 5.77 ATOM 600 CB PRO A 198 1.021 −1.371 5.461 1.00 6.08 ATOM 601 CG PRO A 198 0.279 −0.730 4.269 1.00 6.18 ATOM 602 CD PRO A 198 1.414 −0.399 3.318 1.00 5.79 ATOM 603 C PRO A 198 1.790 −3.489 4.470 1.00 6.13 ATOM 604 O PRO A 198 1.582 −4.338 5.376 1.00 7.38 ATOM 605 N GLY A 199 1.609 −3.797 3.209 1.00 6.60 ATOM 606 CA GLY A 199 1.085 −4.986 2.654 1.00 7.51 ATOM 607 C GLY A 199 −0.337 −4.863 2.155 1.00 7.67 ATOM 608 O GLY A 199 −1.180 −4.373 2.888 1.00 10.17 ATOM 609 N ASP A 200 −0.567 −5.331 0.955 1.00 6.97 ATOM 610 CA ASP A 200 −1.900 −5.225 0.292 1.00 7.36 ATOM 611 CB ASP A 200 −1.784 −4.531 −1.074 1.00 7.25 ATOM 612 CG ASP A 200 −1.028 −5.342 −2.048 1.00 8.64 ATOM 613 OD1 ASP A 200 −0.670 −6.493 −1.749 1.00 9.82 ATOM 614 OD2 ASP A 200 −0.707 −4.901 −3.160 1.00 9.41 ATOM 615 C ASP A 200 −2.657 −6.522 0.241 1.00 7.77 ATOM 616 O ASP A 200 −3.644 −6.653 −0.499 1.00 8.52 ATOM 617 N ALA A 201 −2.262 −7.494 1.053 1.00 6.72 ATOM 618 CA ALA A 201 −3.000 −8.739 1.185 1.00 6.99 ATOM 619 CB ALA A 201 −2.428 −9.661 2.301 1.00 6.57 ATOM 620 C ALA A 201 −4.438 −8.465 1.529 1.00 7.55 ATOM 621 O ALA A 201 −4.767 −7.591 2.280 1.00 8.42 ATOM 622 N VAL A 202 −5.295 −9.319 0.903 1.00 8.04 ATOM 623 CA VAL A 202 −6.745 −9.245 1.124 1.00 9.48 ATOM 624 CB VAL A 202 −7.487 −8.565 −0.054 1.00 10.37 ATOM 625 CG1 VAL A 202 −7.252 −7.081 −0.044 1.00 10.28 ATOM 626 CG2 VAL A 202 −7.166 −9.182 −1.337 1.00 11.04 ATOM 627 C VAL A 202 −7.286 −10.665 1.405 1.00 10.19 ATOM 628 O VAL A 202 −6.903 −11.630 0.831 1.00 10.72 ATOM 629 N ALA A 203 −8.337 −10.618 2.201 1.00 11.68 ATOM 630 CA ALA A 203 −9.123 −11.870 2.504 1.00 13.91 ATOM 631 CB ALA A 203 −9.788 −11.667 3.795 1.00 14.90 ATOM 632 C ALA A 203 −10.113 −12.092 1.404 1.00 15.33 ATOM 633 O ALA A 203 −10.431 −11.146 0.645 1.00 14.65 ATOM 634 N ALA A 204 −10.636 −13.337 1.318 1.00 17.00 ATOM 635 CA ALA A 204 −11.659 −13.634 0.314 1.00 18.07 ATOM 636 CB ALA A 204 −12.202 −15.055 0.542 1.00 19.01 ATOM 637 C ALA A 204 −12.852 −12.673 0.458 1.00 17.51 ATOM 638 O ALA A 204 −13.241 −12.292 1.578 1.00 16.85 ATOM 639 N ASN A 205 −13.256 −12.133 −0.672 1.00 17.92 ATOM 640 CA ASN A 205 −14.397 −11.176 −0.702 1.00 18.82 ATOM 641 CB ASN A 205 −15.666 −11.822 −0.062 1.00 19.75 ATOM 642 CG ASN A 205 −16.040 −13.085 −0.737 1.00 24.23 ATOM 643 OD1 ASN A 205 −16.045 −13.123 −1.928 1.00 21.45 ATOM 644 ND2 ASN A 205 −16.239 −14.161 0.021 1.00 27.35 ATOM 645 C ASN A 205 −14.207 −9.833 −0.064 1.00 19.17 ATOM 646 O ASN A 205 −15.084 −8.968 −0.061 1.00 19.44 ATOM 647 N GLU A 206 −12.964 −9.512 0.331 1.00 14.83 ATOM 648 CA GLU A 206 −12.724 −8.217 0.903 1.00 16.36 ATOM 649 CB GLU A 206 −11.320 −8.148 1.583 1.00 17.79 ATOM 650 CG GLU A 206 −11.096 −6.977 2.445 1.00 19.23 ATOM 651 CD GLU A 206 −9.922 −7.194 3.442 1.00 19.08 ATOM 652 OE1 GLU A 206 −9.146 −8.178 3.344 1.00 17.16 ATOM 653 OE2 GLU A 206 −9.844 −6.347 4.381 1.00 21.73 ATOM 654 C GLU A 206 −12.902 −7.125 −0.125 1.00 15.26 ATOM 655 O GLU A 206 −13.222 −5.958 0.248 1.00 19.72 ATOM 656 N CYS A 207 −12.633 −7.385 −1.359 1.00 14.23 ATOM 657 CA CYS A 207 −12.683 −6.387 −2.452 1.00 16.42 ATOM 658 CB CYS A 207 −11.485 −6.583 −3.314 1.00 14.78 ATOM 659 SG CYS A 207 −9.906 −6.463 −2.453 1.00 14.96 ATOM 660 C CYS A 207 −13.972 −6.570 −3.315 1.00 21.52 ATOM 661 O CYS A 207 −14.048 −6.068 −4.447 1.00 23.46 ATOM 662 N GLY A 208 −14.827 −7.409 −2.785 1.00 20.98 ATOM 663 CA GLY A 208 −16.064 −7.795 −3.503 1.00 23.51 ATOM 664 C GLY A 208 −16.257 −9.282 −3.548 1.00 19.51 ATOM 665 O GLY A 208 −16.884 −9.794 −2.668 1.00 23.61 ATOM 666 N THR A 209 −15.779 −9.922 −4.613 1.00 20.09 ATOM 667 CA THR A 209 −15.871 −11.408 −4.777 1.00 19.12 ATOM 668 CB THR A 209 −16.798 −11.765 −5.955 1.00 24.34 ATOM 669 OG1 THR A 209 −16.213 −11.246 −7.137 1.00 30.57 ATOM 670 CG2 THR A 209 −18.218 −11.124 −5.747 1.00 25.63 ATOM 671 C THR A 209 −14.552 −12.122 −4.996 1.00 23.27 ATOM 672 O THR A 209 −14.493 −13.270 −5.387 1.00 22.39 ATOM 673 N ASN A 210 −13.479 −11.425 −4.709 1.00 20.50 ATOM 674 CA ASN A 210 −12.114 −12.007 −4.882 1.00 17.96 ATOM 675 CB ASN A 210 −11.111 −10.875 −4.568 1.00 17.15 ATOM 676 CG ASN A 210 −11.211 −10.379 −3.163 1.00 16.88 ATOM 677 OD1 ASN A 210 −12.138 −9.682 −2.754 1.00 17.67 ATOM 678 ND2 ASN A 210 −10.232 −10.704 −2.312 1.00 13.84 ATOM 679 C ASN A 210 −11.820 −13.197 −3.975 1.00 14.40 ATOM 680 O ASN A 210 −12.290 −13.338 −2.911 1.00 15.29 ATOM 681 N GLY A 211 −10.912 −14.015 −4.490 1.00 15.74 ATOM 682 CA GLY A 211 −10.299 −14.941 −3.541 1.00 15.89 ATOM 683 C GLY A 211 −9.224 −14.257 −2.694 1.00 14.31 ATOM 684 O GLY A 211 −8.755 −13.134 −3.020 1.00 14.67 ATOM 685 N ALA A 212 −8.816 −14.955 −1.685 1.00 15.00 ATOM 686 CA ALA A 212 −7.728 −14.439 −0.816 1.00 13.95 ATOM 687 CB ALA A 212 −7.520 −15.357 0.424 1.00 15.40 ATOM 688 C ALA A 212 −6.458 −14.293 −1.643 1.00 13.20 ATOM 689 O ALA A 212 −6.124 −15.003 −2.532 1.00 14.94 ATOM 690 N GLN A 213 −5.735 −13.194 −1.352 1.00 11.58 ATOM 691 CA GLN A 213 −4.457 −12.917 −2.032 1.00 11.58 ATOM 692 CB GLN A 213 −4.595 −11.746 −3.048 1.00 13.40 ATOM 693 CG GLN A 213 −5.640 −12.012 −4.165 1.00 19.00 ATOM 694 CD GLN A 213 −6.120 −10.737 −4.871 1.00 28.51 ATOM 695 OE1 GLN A 213 −7.232 −10.715 −5.495 1.00 35.94 ATOM 696 NE2 GLN A 213 −5.326 −9.665 −4.774 1.00 31.68 ATOM 697 C GLN A 213 −3.441 −12.455 −0.982 1.00 9.38 ATOM 698 O GLN A 213 −3.725 −11.654 −0.142 1.00 9.82 ATOM 699 N ASN A 214 −2.241 −12.987 −1.144 1.00 9.02 ATOM 700 CA ASN A 214 −1.097 −12.488 −0.371 1.00 8.55 ATOM 701 CB ASN A 214 0.047 −13.471 −0.498 1.00 10.57 ATOM 702 CG ASN A 214 −0.168 −14.783 0.282 1.00 13.11 ATOM 703 OD1 ASN A 214 −1.064 −14.893 1.082 1.00 14.30 ATOM 704 ND2 ASN A 214 0.800 −15.668 0.170 1.00 19.85 ATOM 705 C ASN A 214 −0.644 −11.131 −0.827 1.00 8.60 ATOM 706 O ASN A 214 −1.024 −10.651 −1.908 1.00 9.05 ATOM 707 N SER A 215 0.153 −10.480 −0.011 1.00 7.14 ATOM 708 CA SER A 215 0.694 −9.161 −0.414 1.00 6.34 ATOM 709 CB SER A 215 1.466 −8.613 0.781 1.00 6.38 ATOM 710 OG SER A 215 0.623 −8.244 1.836 1.00 6.35 ATOM 711 C SER A 215 1.546 −9.291 −1.627 1.00 6.25 ATOM 712 O SER A 215 2.313 −10.176 −1.822 1.00 8.61 ATOM 713 N SER A 216 1.400 −8.279 −2.471 1.00 6.13 ATOM 714 CA SER A 216 2.097 −8.231 −3.739 1.00 6.79 ATOM 715 CB SER A 216 1.371 −7.282 −4.750 1.00 6.97 ATOM 716 OG SER A 216 1.442 −5.968 −4.357 1.00 7.26 ATOM 717 C SER A 216 3.543 −7.770 −3.669 1.00 6.04 ATOM 718 O SER A 216 4.379 −8.114 −4.478 1.00 7.48 ATOM 719 N TRP A 217 3.808 −6.888 −2.701 1.00 5.46 ATOM 720 CA TRP A 217 5.102 −6.148 −2.674 1.00 5.91 ATOM 721 CB TRP A 217 6.241 −6.987 −2.081 1.00 5.66 ATOM 722 CG TRP A 217 5.823 −7.650 −0.842 1.00 5.49 ATOM 723 CD1 TRP A 217 5.701 −9.013 −0.611 1.00 6.05 ATOM 724 NE1 TRP A 217 5.212 −9.248 0.655 1.00 6.18 ATOM 725 CE2 TRP A 217 5.004 −8.053 1.291 1.00 5.67 ATOM 726 CD2 TRP A 217 5.367 −7.023 0.361 1.00 5.54 ATOM 727 CE3 TRP A 217 5.273 −5.695 0.832 1.00 6.38 ATOM 728 CZ3 TRP A 217 4.697 −5.422 2.015 1.00 6.55 ATOM 729 CH2 TRP A 217 4.307 −6.472 2.894 1.00 6.64 ATOM 730 CZ2 TRP A 217 4.459 −7.750 2.537 1.00 6.04 ATOM 731 C TRP A 217 5.460 −5.597 −4.032 1.00 5.23 ATOM 732 O TRP A 217 6.610 −5.453 −4.397 1.00 5.68 ATOM 733 N HIS A 218 4.391 −5.150 −4.783 1.00 5.88 ATOM 734 CA HIS A 218 4.590 −4.827 −6.170 1.00 5.91 ATOM 735 CB HIS A 218 3.157 −4.608 −6.769 1.00 6.76 ATOM 736 CG HIS A 218 3.130 −4.410 −8.217 1.00 6.64 ATOM 737 ND1 HIS A 218 3.128 −3.185 −8.796 1.00 8.19 ATOM 738 CE1 HIS A 218 2.891 −3.302 −10.068 1.00 8.67 ATOM 739 NE2 HIS A 218 2.770 −4.530 −10.362 1.00 10.03 ATOM 740 CD2 HIS A 218 2.906 −5.295 −9.229 1.00 8.13 ATOM 741 C HIS A 218 5.521 −3.631 −6.340 1.00 4.77 ATOM 742 O HIS A 218 6.390 −3.636 −7.225 1.00 4.97 ATOM 743 N GLY A 219 5.312 −2.618 −5.508 1.00 4.47 ATOM 744 CA GLY A 219 6.144 −1.458 −5.636 1.00 4.95 ATOM 745 C GLY A 219 7.618 −1.640 −5.271 1.00 4.49 ATOM 746 O GLY A 219 8.464 −0.930 −5.806 1.00 4.95 ATOM 747 N THR A 220 7.918 −2.630 −4.448 1.00 4.97 ATOM 748 CA THR A 220 9.303 −2.943 −4.105 1.00 4.75 ATOM 749 CB THR A 220 9.300 −3.873 −2.911 1.00 5.04 ATOM 750 OG1 THR A 220 8.735 −3.195 −1.748 1.00 5.77 ATOM 751 CG2 THR A 220 10.720 −4.341 −2.527 1.00 5.11 ATOM 752 C THR A 220 10.003 −3.622 −5.272 1.00 4.30 ATOM 753 O THR A 220 11.116 −3.311 −5.610 1.00 5.06 ATOM 754 N HIS A 221 9.239 −4.476 −5.991 1.00 4.45 ATOM 755 CA HIS A 221 9.742 −5.148 −7.185 1.00 4.74 ATOM 756 CB HIS A 221 8.789 −6.261 −7.559 1.00 4.67 ATOM 757 CG HIS A 221 9.317 −7.267 −8.524 1.00 4.91 ATOM 758 ND1 HIS A 221 9.419 −7.038 −9.851 1.00 5.30 ATOM 759 CE1 HIS A 221 9.994 −8.088 −10.423 1.00 5.39 ATOM 760 NE2 HIS A 221 10.201 −8.989 −9.492 1.00 5.46 ATOM 761 CD2 HIS A 221 9.810 −8.491 −8.295 1.00 5.51 ATOM 762 C HIS A 221 10.010 −4.157 −8.282 1.00 4.16 ATOM 763 O HIS A 221 11.088 −4.055 −8.878 1.00 4.36 ATOM 764 N VAL A 222 9.014 −3.312 −8.566 1.00 4.32 ATOM 765 CA VAL A 222 9.131 −2.278 −9.616 1.00 4.14 ATOM 766 CB VAL A 222 7.751 −1.568 −9.769 1.00 4.18 ATOM 767 CG1 VAL A 222 7.870 −0.344 −10.631 1.00 4.46 ATOM 768 CG2 VAL A 222 6.734 −2.503 −10.345 1.00 4.63 ATOM 769 C VAL A 222 10.238 −1.307 −9.292 1.00 3.91 ATOM 770 O VAL A 222 11.050 −0.965 −10.166 1.00 4.33 ATOM 771 N ALA A 223 10.296 −0.808 −8.057 1.00 4.38 ATOM 772 CA ALA A 223 11.374 0.117 −7.729 1.00 4.85 ATOM 773 CB ALA A 223 11.208 0.687 −6.315 1.00 5.91 ATOM 774 C ALA A 223 12.759 −0.494 −7.922 1.00 4.94 ATOM 775 O ALA A 223 13.680 0.242 −8.289 1.00 5.10 ATOM 776 N GLY A 224 12.897 −1.778 −7.563 1.00 4.62 ATOM 777 CA GLY A 224 14.195 −2.422 −7.784 1.00 4.77 ATOM 778 C GLY A 224 14.589 −2.549 −9.243 1.00 4.86 ATOM 779 O GLY A 224 15.779 −2.438 −9.565 1.00 5.03 ATOM 780 N THR A 225 13.586 −2.731 −10.149 1.00 4.29 ATOM 781 CA THR A 225 13.961 −2.742 −11.536 1.00 4.60 ATOM 782 CB THR A 225 12.741 −3.163 −12.433 1.00 4.81 ATOM 783 OG1 THR A 225 12.367 −4.489 −11.995 1.00 5.17 ATOM 784 CG2 THR A 225 13.039 −3.100 −13.848 1.00 4.90 ATOM 785 C THR A 225 14.464 −1.374 −11.957 1.00 5.08 ATOM 786 O THR A 225 15.437 −1.262 −12.711 1.00 5.31 ATOM 787 N VAL A 226 13.827 −0.323 −11.449 1.00 4.57 ATOM 788 CA VAL A 226 14.330 1.029 −11.809 1.00 4.72 ATOM 789 CB VAL A 226 13.383 2.137 −11.308 1.00 4.59 ATOM 790 CG1 VAL A 226 13.975 3.517 −11.600 1.00 5.81 ATOM 791 CG2 VAL A 226 11.983 1.939 −11.936 1.00 4.58 ATOM 792 C VAL A 226 15.710 1.305 −11.229 1.00 4.90 ATOM 793 O VAL A 226 16.605 1.780 −11.968 1.00 6.04 ATOM 794 N ALA A 227 15.945 0.990 −9.969 1.00 5.55 ATOM 795 CA ALA A 227 17.134 1.499 −9.290 1.00 5.21 ATOM 796 CB ALA A 227 16.935 2.957 −8.911 1.00 6.76 ATOM 797 C ALA A 227 17.574 0.635 −8.072 1.00 5.23 ATOM 798 O ALA A 227 18.111 1.202 −7.102 1.00 6.20 ATOM 799 N ALA A 228 17.555 −0.681 −8.147 1.00 4.90 ATOM 800 CA ALA A 228 18.229 −1.491 −7.130 1.00 5.55 ATOM 801 CB ALA A 228 18.192 −2.954 −7.426 1.00 5.94 ATOM 802 C ALA A 228 19.693 −1.021 −7.093 1.00 6.47 ATOM 803 O ALA A 228 20.330 −0.670 −8.057 1.00 6.25 ATOM 804 N VAL A 229 20.218 −1.123 −5.852 1.00 6.64 ATOM 805 CA VAL A 229 21.624 −0.770 −5.586 1.00 7.17 ATOM 806 CB VAL A 229 21.870 −0.723 −4.112 1.00 7.60 ATOM 807 CG1 VAL A 229 23.399 −0.422 −3.881 1.00 9.16 ATOM 808 CG2 VAL A 229 21.013 0.294 −3.335 1.00 7.45 ATOM 809 C VAL A 229 22.522 −1.777 −6.292 1.00 6.02 ATOM 810 O VAL A 229 22.329 −2.973 −6.121 1.00 6.69 ATOM 811 N THR A 230 23.410 −1.307 −7.115 1.00 6.10 ATOM 812 CA THR A 230 24.166 −2.102 −8.045 1.00 7.34 ATOM 813 CB THR A 230 23.812 −1.660 −9.452 1.00 6.87 ATOM 814 OG1 THR A 230 22.398 −1.910 −9.610 1.00 7.41 ATOM 815 CG2 THR A 230 24.510 −2.400 −10.534 1.00 7.60 ATOM 816 C THR A 230 25.685 −1.964 −7.702 1.00 8.01 ATOM 817 O THR A 230 26.137 −0.947 −7.210 1.00 9.48 ATOM 818 N ASN A 231 26.388 −3.043 −8.058 1.00 8.75 ATOM 819 CA ASN A 231 27.866 −3.114 −7.787 1.00 10.63 ATOM 820 CB ASN A 231 28.624 −1.998 −8.483 1.00 11.86 ATOM 821 CG ASN A 231 28.911 −2.370 −9.833 1.00 15.66 ATOM 822 OD1 ASN A 231 29.324 −3.518 −10.096 1.00 19.43 ATOM 823 ND2 ASN A 231 28.782 −1.396 −10.709 1.00 18.84 ATOM 824 C ASN A 231 28.129 −3.131 −6.373 1.00 12.07 ATOM 825 O ASN A 231 29.251 −2.726 −5.961 1.00 14.30 ATOM 826 N ASN A 232 27.231 −3.601 −5.538 1.00 8.94 ATOM 827 CA ASN A 232 27.419 −3.765 −4.096 1.00 9.39 ATOM 828 CB ASN A 232 26.302 −3.087 −3.269 1.00 9.14 ATOM 829 CG ASN A 232 24.936 −3.758 −3.486 1.00 7.37 ATOM 830 OD1 ASN A 232 24.722 −4.465 −4.495 1.00 8.78 ATOM 831 ND2 ASN A 232 24.059 −3.607 −2.538 1.00 8.00 ATOM 832 C ASN A 232 27.652 −5.202 −3.670 1.00 8.81 ATOM 833 O ASN A 232 27.538 −5.537 −2.506 1.00 10.78 ATOM 834 N GLY A 233 27.871 −6.098 −4.632 1.00 9.35 ATOM 835 CA GLY A 233 28.127 −7.448 −4.304 1.00 10.02 ATOM 836 C GLY A 233 27.087 −8.256 −3.714 1.00 10.05 ATOM 837 O GLY A 233 27.269 −9.339 −3.113 1.00 12.28 ATOM 838 N GLU A 234 25.813 −7.749 −3.841 1.00 10.22 ATOM 839 CA GLU A 234 24.679 −8.288 −3.156 1.00 10.60 ATOM 840 CB GLU A 234 24.302 −7.434 −1.923 1.00 13.14 ATOM 841 CG GLU A 234 23.161 −7.904 −1.128 1.00 15.74 ATOM 842 CD GLU A 234 22.913 −7.005 0.140 1.00 18.30 ATOM 843 OE1 GLU A 234 21.922 −7.310 0.863 1.00 19.80 ATOM 844 OE2 GLU A 234 23.733 −6.063 0.356 1.00 20.57 ATOM 845 C GLU A 234 23.447 −8.305 −4.109 1.00 8.14 ATOM 846 O GLU A 234 23.282 −7.327 −4.814 1.00 8.02 ATOM 847 N GLY A 235 22.679 −9.335 −4.106 1.00 7.54 ATOM 848 CA GLY A 235 21.354 −9.286 −4.757 1.00 7.74 ATOM 849 C GLY A 235 21.495 −9.012 −6.228 1.00 7.31 ATOM 850 O GLY A 235 22.250 −9.618 −7.007 1.00 7.35 ATOM 851 N VAL A 236 20.648 −8.080 −6.681 1.00 6.74 ATOM 852 CA VAL A 236 20.372 −7.701 −8.072 1.00 6.15 ATOM 853 CB VAL A 236 18.806 −7.744 −8.309 1.00 6.57 ATOM 854 CG1 VAL A 236 18.238 −9.127 −8.145 1.00 7.27 ATOM 855 CG2 VAL A 236 18.126 −6.754 −7.397 1.00 6.46 ATOM 856 C VAL A 236 20.946 −6.398 −8.534 1.00 5.64 ATOM 857 O VAL A 236 21.523 −5.647 −7.663 1.00 6.39 ATOM 858 N ALA A 237 20.770 −6.064 −9.815 1.00 5.71 ATOM 859 CA ALA A 237 21.051 −4.773 −10.332 1.00 5.75 ATOM 860 CB ALA A 237 22.013 −4.879 −11.501 1.00 7.74 ATOM 861 C ALA A 237 19.775 −4.055 −10.737 1.00 6.15 ATOM 862 O ALA A 237 18.715 −4.672 −10.840 1.00 6.36 ATOM 863 N GLY A 238 19.908 −2.747 −10.899 1.00 5.24 ATOM 864 CA GLY A 238 18.806 −1.887 −11.354 1.00 5.24 ATOM 865 C GLY A 238 19.161 −1.313 −12.698 1.00 5.44 ATOM 866 O GLY A 238 20.319 −1.162 −13.094 1.00 6.90 ATOM 867 N VAL A 239 18.159 −0.906 −13.500 1.00 5.05 ATOM 868 CA VAL A 239 18.389 −0.348 −14.831 1.00 5.73 ATOM 869 CB VAL A 239 17.035 −0.260 −15.579 1.00 5.25 ATOM 870 CG1 VAL A 239 17.203 0.460 −16.909 1.00 5.92 ATOM 871 CG2 VAL A 239 16.449 −1.641 −15.779 1.00 5.74 ATOM 872 C VAL A 239 19.085 0.968 −14.764 1.00 5.55 ATOM 873 O VAL A 239 20.015 1.221 −15.543 1.00 6.81 ATOM 874 N ALA A 240 18.580 1.874 −13.929 1.00 5.48 ATOM 875 CA ALA A 240 19.144 3.205 −13.737 1.00 6.06 ATOM 876 CB ALA A 240 18.146 4.300 −14.029 1.00 6.87 ATOM 877 C ALA A 240 19.676 3.268 −12.321 1.00 5.93 ATOM 878 O ALA A 240 19.190 4.006 −11.481 1.00 6.15 ATOM 879 N TYR A 241 20.769 2.516 −12.067 1.00 6.17 ATOM 880 CA TYR A 241 21.204 2.225 −10.719 1.00 6.10 ATOM 881 CB TYR A 241 22.053 0.970 −10.673 1.00 6.60 ATOM 882 CG TYR A 241 23.276 0.981 −11.532 1.00 7.15 ATOM 883 CD1 TYR A 241 24.442 1.663 −11.121 1.00 8.13 ATOM 884 CE1 TYR A 241 25.635 1.581 −11.866 1.00 8.02 ATOM 885 CZ TYR A 241 25.628 0.836 −12.976 1.00 8.52 ATOM 886 OH TYR A 241 26.797 0.672 −13.728 1.00 10.69 ATOM 887 CE2 TYR A 241 24.492 0.212 −13.433 1.00 8.91 ATOM 888 CD2 TYR A 241 23.349 0.278 −12.677 1.00 7.50 ATOM 889 C TYR A 241 21.880 3.389 −9.996 1.00 6.07 ATOM 890 O TYR A 241 22.047 3.288 −8.742 1.00 6.86 ATOM 891 N ASN A 242 22.190 4.459 −10.689 1.00 6.15 ATOM 892 CA ASN A 242 22.674 5.696 −9.982 1.00 7.26 ATOM 893 CB ASN A 242 23.967 6.190 −10.612 1.00 7.71 ATOM 894 CG ASN A 242 25.166 5.397 −10.213 1.00 8.57 ATOM 895 OD1 ASN A 242 25.253 4.843 −9.135 1.00 10.45 ATOM 896 ND2 ASN A 242 26.039 5.196 −11.172 1.00 9.24 ATOM 897 C ASN A 242 21.623 6.772 −9.950 1.00 7.31 ATOM 898 O ASN A 242 21.867 7.848 −9.450 1.00 8.52 ATOM 899 N ALA A 243 20.370 6.473 −10.424 1.00 6.93 ATOM 900 CA ALA A 243 19.236 7.348 −10.188 1.00 6.35 ATOM 901 CB ALA A 243 18.137 7.068 −11.186 1.00 6.56 ATOM 902 C ALA A 243 18.768 7.155 −8.768 1.00 7.21 ATOM 903 O ALA A 243 19.207 6.285 −8.035 1.00 7.50 ATOM 904 N LYS A 244 17.778 7.930 −8.366 1.00 6.74 ATOM 905 CA LYS A 244 17.078 7.768 −7.108 1.00 7.56 ATOM 906 CB LYS A 244 17.296 8.944 −6.165 1.00 10.32 ATOM 907 CG LYS A 244 18.718 9.160 −5.833 1.00 13.60 ATOM 908 CD LYS A 244 19.279 8.077 −4.980 1.00 17.31 ATOM 909 CE LYS A 244 20.665 8.533 −4.375 1.00 25.46 ATOM 910 NZ LYS A 244 21.456 7.272 −4.249 1.00 33.20 ATOM 911 C LYS A 244 15.568 7.660 −7.355 1.00 6.32 ATOM 912 O LYS A 244 15.065 8.258 −8.371 1.00 6.92 ATOM 913 N VAL A 245 14.894 6.901 −6.522 1.00 5.87 ATOM 914 CA VAL A 245 13.451 6.669 −6.613 1.00 6.25 ATOM 915 CB VAL A 245 13.129 5.203 −6.562 1.00 6.74 ATOM 916 CG1 VAL A 245 11.625 4.919 −6.341 1.00 6.97 ATOM 917 CG2 VAL A 245 13.567 4.515 −7.826 1.00 7.77 ATOM 918 C VAL A 245 12.704 7.407 −5.537 1.00 6.74 ATOM 919 O VAL A 245 13.060 7.304 −4.350 1.00 6.32 ATOM 920 N VAL A 246 11.691 8.148 −5.974 1.00 5.40 ATOM 921 CA VAL A 246 10.702 8.735 −5.028 1.00 5.84 ATOM 922 CB VAL A 246 10.276 10.136 −5.427 1.00 5.85 ATOM 923 CG1 VAL A 246 9.120 10.656 −4.638 1.00 6.06 ATOM 924 CG2 VAL A 246 11.456 11.072 −5.307 1.00 6.78 ATOM 925 C VAL A 246 9.522 7.797 −5.036 1.00 5.38 ATOM 926 O VAL A 246 8.811 7.636 −6.059 1.00 5.68 ATOM 927 N PRO A 247 9.212 7.128 −3.950 1.00 5.41 ATOM 928 CA PRO A 247 8.066 6.263 −3.851 1.00 5.68 ATOM 929 CB PRO A 247 8.388 5.348 −2.669 1.00 6.41 ATOM 930 CG PRO A 247 9.232 6.241 −1.764 1.00 6.73 ATOM 931 CD PRO A 247 10.017 7.145 −2.677 1.00 6.06 ATOM 932 C PRO A 247 6.803 7.032 −3.600 1.00 4.78 ATOM 933 O PRO A 247 6.749 7.810 −2.669 1.00 5.49 ATOM 934 N VAL A 248 5.816 6.898 −4.473 1.00 5.14 ATOM 935 CA VAL A 248 4.527 7.567 −4.336 1.00 5.39 ATOM 936 CB VAL A 248 4.259 8.530 −5.503 1.00 5.52 ATOM 937 CG1 VAL A 248 2.982 9.311 −5.251 1.00 6.62 ATOM 938 CG2 VAL A 248 5.411 9.466 −5.697 1.00 6.19 ATOM 939 C VAL A 248 3.529 6.498 −4.189 1.00 5.25 ATOM 940 O VAL A 248 3.206 5.770 −5.143 1.00 6.04 ATOM 941 N ARG A 249 3.022 6.306 −2.961 1.00 5.06 ATOM 942 CA ARG A 249 2.142 5.228 −2.653 1.00 5.32 ATOM 943 CB ARG A 249 2.250 4.745 −1.198 1.00 5.83 ATOM 944 CG ARG A 249 1.282 3.682 −0.862 1.00 5.96 ATOM 945 CD ARG A 249 1.664 2.996 0.449 1.00 5.65 ATOM 946 NE ARG A 249 0.755 1.895 0.658 1.00 5.98 ATOM 947 CZ ARG A 249 −0.264 1.790 1.514 1.00 5.77 ATOM 948 NH1 ARG A 249 −0.504 2.756 2.365 1.00 7.32 ATOM 949 NH2 ARG A 249 −1.049 0.742 1.494 1.00 6.35 ATOM 950 C ARG A 249 0.710 5.615 −3.046 1.00 5.14 ATOM 951 O ARG A 249 0.134 6.509 −2.438 1.00 5.55 ATOM 952 N VAL A 250 0.153 4.900 −4.011 1.00 5.71 ATOM 953 CA VAL A 250 −1.186 5.154 −4.506 1.00 5.93 ATOM 954 CB VAL A 250 −1.143 5.824 −5.910 1.00 6.23 ATOM 955 CG1 VAL A 250 −0.352 7.153 −5.896 1.00 6.88 ATOM 956 CG2 VAL A 250 −0.590 4.890 −6.965 1.00 5.83 ATOM 957 C VAL A 250 −2.050 3.943 −4.545 1.00 6.27 ATOM 958 O VAL A 250 −3.280 4.094 −4.753 1.00 6.95 ATOM 959 N LEU A 251 −1.527 2.764 −4.292 1.00 5.77 ATOM 960 CA LEU A 251 −2.250 1.475 −4.325 1.00 6.61 ATOM 961 CB LEU A 251 −1.692 0.533 −5.378 1.00 7.12 ATOM 962 CG LEU A 251 −1.510 1.066 −6.805 1.00 7.36 ATOM 963 CD1 LEU A 251 −0.864 0.008 −7.698 1.00 8.31 ATOM 964 CD2 LEU A 251 −2.866 1.499 −7.390 1.00 10.10 ATOM 965 C LEU A 251 −2.116 0.786 −2.982 1.00 6.74 ATOM 966 O LEU A 251 −1.023 0.703 −2.413 1.00 6.44 ATOM 967 N GLY A 252 −3.245 0.314 −2.478 1.00 7.06 ATOM 968 CA GLY A 252 −3.321 −0.484 −1.271 1.00 6.95 ATOM 969 C GLY A 252 −4.178 −1.666 −1.401 1.00 6.86 ATOM 970 O GLY A 252 −4.266 −2.329 −2.458 1.00 7.08 ATOM 971 N LYS A 253 −4.880 −2.019 −0.300 1.00 7.18 ATOM 972 CA LYS A 253 −5.773 −3.205 −0.394 1.00 8.36 ATOM 973 CB LYS A 253 −6.380 −3.473 0.962 1.00 9.35 ATOM 974 CG LYS A 253 −5.361 −3.949 2.003 1.00 9.58 ATOM 975 CD LYS A 253 −5.917 −4.004 3.419 1.00 11.68 ATOM 976 CE LYS A 253 −6.982 −5.042 3.527 1.00 13.74 ATOM 977 NZ LYS A 253 −7.499 −5.202 4.949 1.00 15.16 ATOM 978 C LYS A 253 −6.914 −2.975 −1.384 1.00 7.99 ATOM 979 O LYS A 253 −7.608 −1.933 −1.254 1.00 10.37 ATOM 980 N CYS A 254 −7.024 −3.862 −2.323 1.00 9.18 ATOM 981 CA CYS A 254 −8.081 −3.798 −3.389 1.00 10.97 ATOM 982 CB CYS A 254 −9.488 −3.453 −2.857 1.00 12.26 ATOM 983 SG CYS A 254 −9.985 −4.581 −1.550 1.00 14.67 ATOM 984 C CYS A 254 −7.700 −2.814 −4.479 1.00 12.69 ATOM 985 O CYS A 254 −8.509 −2.689 −5.457 1.00 16.80 ATOM 986 N GLY A 255 −6.527 −2.197 −4.499 1.00 9.49 ATOM 987 CA GLY A 255 −6.099 −1.337 −5.592 1.00 10.14 ATOM 988 C GLY A 255 −6.025 0.075 −5.130 1.00 9.93 ATOM 989 O GLY A 255 −5.648 0.412 −4.033 1.00 8.93 ATOM 990 N GLY A 256 −6.324 1.050 −6.063 1.00 9.91 ATOM 991 CA GLY A 256 −6.245 2.465 −5.832 1.00 9.93 ATOM 992 C GLY A 256 −7.328 3.265 −6.502 1.00 10.09 ATOM 993 O GLY A 256 −7.936 2.762 −7.443 1.00 13.46 ATOM 994 N LEU A 257 −7.527 4.414 −5.955 1.00 9.25 ATOM 995 CA LEU A 257 −8.531 5.361 −6.468 1.00 8.95 ATOM 996 CB LEU A 257 −8.953 6.221 −5.334 1.00 12.19 ATOM 997 CG LEU A 257 −10.129 7.202 −5.512 1.00 18.10 ATOM 998 CD1 LEU A 257 −11.412 6.505 −6.019 1.00 21.85 ATOM 999 CD2 LEU A 257 −10.460 7.968 −4.192 1.00 22.02 ATOM 1000 C LEU A 257 −7.926 6.243 −7.558 1.00 8.83 ATOM 1001 O LEU A 257 −6.841 6.820 −7.352 1.00 8.21 ATOM 1002 N THR A 258 −8.652 6.417 −8.641 1.00 9.81 ATOM 1003 CA THR A 258 −8.236 7.302 −9.716 1.00 10.64 ATOM 1004 CB THR A 258 −9.387 7.434 −10.785 1.00 12.32 ATOM 1005 OG1 THR A 258 −9.677 6.104 −11.189 1.00 17.49 ATOM 1006 CG2 THR A 258 −8.946 8.382 −11.935 1.00 14.19 ATOM 1007 C THR A 258 −7.790 8.649 −9.218 1.00 9.03 ATOM 1008 O THR A 258 −6.715 9.187 −9.662 1.00 8.12 ATOM 1009 N SER A 259 −8.480 9.287 −8.299 1.00 9.39 ATOM 1010 CA SER A 259 −8.152 10.570 −7.861 1.00 10.48 ATOM 1011 CB SER A 259 −9.314 11.279 −7.102 1.00 14.15 ATOM 1012 OG SER A 259 −9.714 10.414 −6.132 1.00 16.77 ATOM 1013 C SER A 259 −6.844 10.661 −7.010 1.00 9.02 ATOM 1014 O SER A 259 −6.121 11.639 −7.080 1.00 10.05 ATOM 1015 N ASP A 260 −6.604 9.566 −6.243 1.00 8.17 ATOM 1016 CA ASP A 260 −5.349 9.490 −5.520 1.00 7.71 ATOM 1017 CB ASP A 260 −5.378 8.342 −4.527 1.00 7.43 ATOM 1018 CG ASP A 260 −6.327 8.541 −3.335 1.00 8.37 ATOM 1019 OD1 ASP A 260 −6.692 9.689 −3.015 1.00 9.60 ATOM 1020 OD2 ASP A 260 −6.535 7.526 −2.645 1.00 8.36 ATOM 1021 C ASP A 260 −4.182 9.290 −6.488 1.00 6.53 ATOM 1022 O ASP A 260 −3.109 9.899 −6.285 1.00 7.09 ATOM 1023 N ILE A 261 −4.336 8.449 −7.464 1.00 6.25 ATOM 1024 CA ILE A 261 −3.303 8.244 −8.489 1.00 6.08 ATOM 1025 CB ILE A 261 −3.704 7.131 −9.455 1.00 6.71 ATOM 1026 CG1 ILE A 261 −3.840 5.824 −8.664 1.00 6.87 ATOM 1027 CD1 ILE A 261 −4.616 4.751 −9.389 1.00 8.00 ATOM 1028 CG2 ILE A 261 −2.734 7.056 −10.585 1.00 7.12 ATOM 1029 C ILE A 261 −2.984 9.544 −9.180 1.00 6.73 ATOM 1030 O ILE A 261 −1.813 9.895 −9.401 1.00 6.78 ATOM 1031 N ALA A 262 −4.016 10.288 −9.542 1.00 6.85 ATOM 1032 CA ALA A 262 −3.826 11.564 −10.259 1.00 6.67 ATOM 1033 CB ALA A 262 −5.174 12.082 −10.732 1.00 8.05 ATOM 1034 C ALA A 262 −3.107 12.575 −9.408 1.00 6.45 ATOM 1035 O ALA A 262 −2.175 13.253 −9.874 1.00 6.76 ATOM 1036 N ASP A 263 −3.450 12.694 −8.123 1.00 6.58 ATOM 1037 CA ASP A 263 −2.701 13.562 −7.228 1.00 6.94 ATOM 1038 CB ASP A 263 −3.420 13.706 −5.908 1.00 8.02 ATOM 1039 CG ASP A 263 −4.623 14.581 −5.966 1.00 8.36 ATOM 1040 OD1 ASP A 263 −4.750 15.377 −6.937 1.00 10.85 ATOM 1041 OD2 ASP A 263 −5.415 14.551 −5.005 1.00 9.59 ATOM 1042 C ASP A 263 −1.229 13.139 −7.063 1.00 6.95 ATOM 1043 O ASP A 263 −0.307 13.932 −6.949 1.00 6.77 ATOM 1044 N GLY A 264 −1.038 11.793 −7.106 1.00 5.92 ATOM 1045 CA GLY A 264 0.317 11.300 −7.125 1.00 5.79 ATOM 1046 C GLY A 264 1.169 11.740 −8.312 1.00 5.75 ATOM 1047 O GLY A 264 2.314 12.095 −8.173 1.00 6.19 ATOM 1048 N ILE A 265 0.509 11.693 −9.486 1.00 5.47 ATOM 1049 CA ILE A 265 1.179 12.139 −10.729 1.00 5.79 ATOM 1050 CB ILE A 265 0.250 11.968 −11.930 1.00 5.68 ATOM 1051 CG1 ILE A 265 −0.031 10.446 −12.213 1.00 6.12 ATOM 1052 CD1 ILE A 265 −1.150 10.186 −13.166 1.00 6.43 ATOM 1053 CG2 ILE A 265 0.761 12.678 −13.144 1.00 5.98 ATOM 1054 C ILE A 265 1.550 13.628 −10.605 1.00 6.09 ATOM 1055 O ILE A 265 2.697 14.018 −10.840 1.00 6.12 ATOM 1056 N ILE A 266 0.580 14.410 −10.163 1.00 5.82 ATOM 1057 CA ILE A 266 0.848 15.876 −9.992 1.00 6.32 ATOM 1058 CB ILE A 266 −0.452 16.574 −9.515 1.00 6.83 ATOM 1059 CG1 ILE A 266 −1.523 16.502 −10.585 1.00 7.13 ATOM 1060 CD1 ILE A 266 −2.888 16.779 −10.035 1.00 8.04 ATOM 1061 CG2 ILE A 266 −0.152 18.054 −9.109 1.00 7.65 ATOM 1062 C ILE A 266 2.019 16.137 −9.023 1.00 6.10 ATOM 1063 O ILE A 266 2.986 16.814 −9.370 1.00 6.21 ATOM 1064 N TRP A 267 1.896 15.533 −7.838 1.00 5.45 ATOM 1065 CA TRP A 267 2.916 15.792 −6.811 1.00 6.03 ATOM 1066 CB TRP A 267 2.433 15.143 −5.472 1.00 6.31 ATOM 1067 CG TRP A 267 3.353 15.405 −4.313 1.00 6.84 ATOM 1068 CD1 TRP A 267 3.368 16.549 −3.498 1.00 7.96 ATOM 1069 NE1 TRP A 267 4.337 16.395 −2.573 1.00 9.06 ATOM 1070 CE2 TRP A 267 5.048 15.255 −2.758 1.00 7.31 ATOM 1071 CD2 TRP A 267 4.424 14.590 −3.844 1.00 7.20 ATOM 1072 CE3 TRP A 267 4.960 13.358 −4.257 1.00 7.18 ATOM 1073 CZ3 TRP A 267 6.030 12.865 −3.566 1.00 7.97 ATOM 1074 CH2 TRP A 267 6.602 13.549 −2.484 1.00 7.66 ATOM 1075 CZ2 TRP A 267 6.089 14.687 −2.055 1.00 8.56 ATOM 1076 C TRP A 267 4.290 15.297 −7.237 1.00 6.14 ATOM 1077 O TRP A 267 5.319 15.894 −6.963 1.00 7.03 ATOM 1078 N ALA A 268 4.324 14.095 −7.849 1.00 5.33 ATOM 1079 CA ALA A 268 5.563 13.481 −8.266 1.00 5.53 ATOM 1080 CB ALA A 268 5.316 12.200 −9.003 1.00 5.81 ATOM 1081 C ALA A 268 6.324 14.384 −9.196 1.00 5.99 ATOM 1082 O ALA A 268 7.562 14.427 −9.189 1.00 6.17 ATOM 1083 N SER A 269 5.587 15.152 −10.034 1.00 5.83 ATOM 1084 CA SER A 269 6.202 16.072 −10.995 1.00 6.89 ATOM 1085 CB SER A 269 5.235 16.305 −12.143 1.00 6.90 ATOM 1086 OG SER A 269 4.220 17.243 −11.857 1.00 7.31 ATOM 1087 C SER A 269 6.648 17.405 −10.385 1.00 8.30 ATOM 1088 O SER A 269 7.193 18.222 −11.158 1.00 9.78 ATOM 1089 N GLY A 270 6.303 17.649 −9.143 1.00 7.39 ATOM 1090 CA GLY A 270 6.600 18.935 −8.507 1.00 8.65 ATOM 1091 C GLY A 270 5.433 19.840 −8.555 1.00 10.47 ATOM 1092 O GLY A 270 5.574 21.082 −8.253 1.00 11.96 ATOM 1093 N GLY A 271 4.254 19.401 −8.916 1.00 9.96 ATOM 1094 CA GLY A 271 3.096 20.224 −8.826 1.00 9.95 ATOM 1095 C GLY A 271 2.500 20.223 −7.462 1.00 11.79 ATOM 1096 O GLY A 271 2.798 19.395 −6.600 1.00 13.48 ATOM 1097 N SER A 272 1.552 21.106 −7.238 1.00 13.99 ATOM 1098 CA SER A 272 0.959 21.296 −5.925 1.00 14.15 ATOM 1099 CB SER A 272 0.788 22.819 −5.639 1.00 17.92 ATOM 1100 OG SER A 272 0.229 23.073 −4.325 1.00 26.73 ATOM 1101 C SER A 272 −0.358 20.554 −5.773 1.00 13.01 ATOM 1102 O SER A 272 −1.188 20.583 −6.699 1.00 15.70 ATOM 1103 N VAL A 273 −0.539 19.909 −4.610 1.00 10.80 ATOM 1104 CA VAL A 273 −1.756 19.215 −4.237 1.00 11.04 ATOM 1105 CB VAL A 273 −1.535 17.679 −4.149 1.00 10.96 ATOM 1106 CG1 VAL A 273 −2.728 16.946 −3.608 1.00 11.20 ATOM 1107 CG2 VAL A 273 −1.057 17.164 −5.507 1.00 11.40 ATOM 1108 C VAL A 273 −2.130 19.836 −2.918 1.00 12.31 ATOM 1109 O VAL A 273 −1.372 19.902 −1.983 1.00 11.77 ATOM 1110 N SER A 274 −3.409 20.202 −2.826 1.00 14.45 ATOM 1111 CA SER A 274 −3.909 20.763 −1.522 1.00 15.38 ATOM 1112 CB SER A 274 −5.406 20.989 −1.732 1.00 15.81 ATOM 1113 OG SER A 274 −5.696 21.991 −2.743 1.00 26.14 ATOM 1114 C SER A 274 −3.710 19.802 −0.331 1.00 13.55 ATOM 1115 O SER A 274 −3.930 18.639 −0.589 1.00 21.53 ATOM 1116 N GLY A 275 −3.094 20.354 0.717 1.00 18.38 ATOM 1117 CA GLY A 275 −2.880 19.649 1.986 1.00 17.94 ATOM 1118 C GLY A 275 −1.596 18.853 1.990 1.00 19.27 ATOM 1119 O GLY A 275 −1.263 18.225 2.966 1.00 23.01 ATOM 1120 N ILE A 276 −0.790 18.940 0.942 1.00 14.27 ATOM 1121 CA ILE A 276 0.403 18.122 0.885 1.00 13.55 ATOM 1122 CB ILE A 276 0.412 17.199 −0.358 1.00 14.72 ATOM 1123 CG1 ILE A 276 −0.829 16.308 −0.406 1.00 16.48 ATOM 1124 CD1 ILE A 276 −0.730 15.235 −1.457 1.00 18.90 ATOM 1125 CG2 ILE A 276 1.661 16.308 −0.382 1.00 14.09 ATOM 1126 C ILE A 276 1.625 19.086 0.808 1.00 11.46 ATOM 1127 O ILE A 276 1.686 19.904 −0.111 1.00 12.23 ATOM 1128 N PRO A 277 2.659 18.837 1.620 1.00 13.41 ATOM 1129 CA PRO A 277 3.837 19.658 1.483 1.00 13.72 ATOM 1130 CB PRO A 277 4.772 19.154 2.604 1.00 16.49 ATOM 1131 CG PRO A 277 3.993 18.157 3.412 1.00 19.44 ATOM 1132 CD PRO A 277 2.711 17.841 2.700 1.00 16.91 ATOM 1133 C PRO A 277 4.524 19.615 0.062 1.00 11.88 ATOM 1134 O PRO A 277 4.476 18.481 −0.693 1.00 13.42 ATOM 1135 N ALA A 278 5.185 20.636 −0.371 1.00 15.17 ATOM 1136 CA ALA A 278 5.785 20.612 −1.660 1.00 15.11 ATOM 1137 CB ALA A 278 6.511 21.936 −1.947 1.00 18.13 ATOM 1138 C ALA A 278 6.790 19.482 −1.759 1.00 13.79 ATOM 1139 O ALA A 278 7.515 19.149 −0.841 1.00 14.07 ATOM 1140 N ASN A 279 6.866 18.855 −2.928 1.00 12.14 ATOM 1141 CA ASN A 279 7.859 17.804 −3.184 1.00 10.11 ATOM 1142 CB ASN A 279 7.411 17.051 −4.414 1.00 8.65 ATOM 1143 CG ASN A 279 8.240 15.817 −4.666 1.00 7.63 ATOM 1144 OD1 ASN A 279 9.254 15.517 −4.022 1.00 8.87 ATOM 1145 ND2 ASN A 279 7.846 15.091 −5.700 1.00 8.18 ATOM 1146 C ASN A 279 9.268 18.381 −3.356 1.00 10.02 ATOM 1147 O ASN A 279 9.491 19.134 −4.324 1.00 12.54 ATOM 1148 N SER A 280 10.192 18.052 −2.457 1.00 10.05 ATOM 1149 CA SER A 280 11.495 18.549 −2.626 1.00 11.95 ATOM 1150 CB SER A 280 12.275 18.733 −1.358 1.00 17.79 ATOM 1151 OG SER A 280 12.239 17.687 −0.676 1.00 22.04 ATOM 1152 C SER A 280 12.368 17.842 −3.650 1.00 11.25 ATOM 1153 O SER A 280 13.473 18.263 −3.986 1.00 12.26 ATOM 1154 N ASN A 281 11.851 16.723 −4.230 1.00 9.31 ATOM 1155 CA ASN A 281 12.519 15.954 −5.198 1.00 9.21 ATOM 1156 CB ASN A 281 12.989 14.617 −4.612 1.00 9.81 ATOM 1157 CG ASN A 281 13.889 14.777 −3.391 1.00 10.78 ATOM 1158 OD1 ASN A 281 15.049 15.136 −3.639 1.00 12.93 ATOM 1159 ND2 ASN A 281 13.393 14.466 −2.242 1.00 10.82 ATOM 1160 C ASN A 281 11.655 15.719 −6.419 1.00 9.16 ATOM 1161 O ASN A 281 11.289 14.537 −6.701 1.00 8.57 ATOM 1162 N PRO A 282 11.300 16.743 −7.133 1.00 8.30 ATOM 1163 CA PRO A 282 10.492 16.544 −8.349 1.00 8.28 ATOM 1164 CB PRO A 282 10.342 17.939 −8.996 1.00 9.66 ATOM 1165 CG PRO A 282 11.444 18.719 −8.327 1.00 12.41 ATOM 1166 CD PRO A 282 11.756 18.144 −6.985 1.00 10.17 ATOM 1167 C PRO A 282 11.168 15.565 −9.278 1.00 8.13 ATOM 1168 O PRO A 282 12.360 15.534 −9.457 1.00 9.78 ATOM 1169 N ALA A 283 10.301 14.746 −9.919 1.00 6.74 ATOM 1170 CA ALA A 283 10.774 13.627 −10.762 1.00 6.94 ATOM 1171 CB ALA A 283 9.833 12.452 −10.671 1.00 7.83 ATOM 1172 C ALA A 283 11.005 14.021 −12.180 1.00 6.35 ATOM 1173 O ALA A 283 10.242 14.783 −12.758 1.00 8.11 ATOM 1174 N ASP A 284 12.017 13.476 −12.788 1.00 6.31 ATOM 1175 CA ASP A 284 12.225 13.493 −14.216 1.00 7.04 ATOM 1176 CB ASP A 284 13.699 13.242 −14.520 1.00 7.44 ATOM 1177 CG ASP A 284 14.611 14.165 −13.785 1.00 9.45 ATOM 1178 OD1 ASP A 284 14.612 15.324 −14.180 1.00 17.29 ATOM 1179 OD2 ASP A 284 15.242 13.874 −12.775 1.00 9.56 ATOM 1180 C ASP A 284 11.382 12.496 −15.008 1.00 5.94 ATOM 1181 O ASP A 284 11.002 12.712 −16.142 1.00 6.32 ATOM 1182 N VAL A 285 11.068 11.418 −14.313 1.00 5.19 ATOM 1183 CA VAL A 285 10.379 10.261 −14.950 1.00 4.89 ATOM 1184 CB VAL A 285 11.347 9.114 −15.228 1.00 5.45 ATOM 1185 CG1 VAL A 285 10.636 7.921 −15.869 1.00 5.56 ATOM 1186 CG2 VAL A 285 12.519 9.570 −16.032 1.00 5.70 ATOM 1187 C VAL A 285 9.315 9.792 −13.935 1.00 4.48 ATOM 1188 O VAL A 285 9.656 9.702 −12.747 1.00 5.18 ATOM 1189 N ILE A 286 8.120 9.476 −14.400 1.00 4.40 ATOM 1190 CA ILE A 286 7.099 8.761 −13.620 1.00 4.82 ATOM 1191 CB ILE A 286 5.776 9.499 −13.627 1.00 5.15 ATOM 1192 CG1 ILE A 286 5.846 10.743 −12.668 1.00 5.83 ATOM 1193 CD1 ILE A 286 4.742 11.702 −12.908 1.00 7.06 ATOM 1194 CG2 ILE A 286 4.594 8.652 −13.331 1.00 5.68 ATOM 1195 C ILE A 286 6.966 7.407 −14.206 1.00 4.95 ATOM 1196 O ILE A 286 6.829 7.231 −15.439 1.00 5.80 ATOM 1197 N ASN A 287 6.925 6.392 −13.349 1.00 4.39 ATOM 1198 CA ASN A 287 6.522 5.010 −13.706 1.00 4.73 ATOM 1199 CB ASN A 287 7.625 4.020 −13.263 1.00 4.91 ATOM 1200 CG ASN A 287 7.327 2.629 −13.726 1.00 5.06 ATOM 1201 OD1 ASN A 287 7.750 2.246 −14.821 1.00 6.39 ATOM 1202 ND2 ASN A 287 6.538 1.866 −12.951 1.00 5.38 ATOM 1203 C ASN A 287 5.197 4.671 −13.082 1.00 4.41 ATOM 1204 O ASN A 287 5.037 4.781 −11.856 1.00 5.46 ATOM 1205 N MET A 288 4.245 4.232 −13.892 1.00 4.75 ATOM 1206 CA MET A 288 2.952 3.743 −13.423 1.00 5.21 ATOM 1207 CB MET A 288 1.743 4.591 −13.902 1.00 6.62 ATOM 1208 CG MET A 288 1.687 5.883 −13.083 1.00 8.49 ATOM 1209 SD MET A 288 0.094 6.772 −13.290 1.00 13.07 ATOM 1210 CE MET A 288 0.844 7.333 −14.427 1.00 4.78 ATOM 1211 C MET A 288 2.757 2.360 −13.950 1.00 4.85 ATOM 1212 O MET A 288 2.243 2.064 −15.069 1.00 5.84 ATOM 1213 N SER A 289 3.062 1.371 −13.093 1.00 5.39 ATOM 1214 CA SER A 289 2.854 −0.079 −13.357 1.00 5.94 ATOM 1215 CB SER A 289 3.985 −0.840 −12.698 1.00 5.58 ATOM 1216 OG SER A 289 5.173 −0.729 −13.441 1.00 5.46 ATOM 1217 C SER A 289 1.508 −0.440 −12.789 1.00 6.76 ATOM 1218 O SER A 289 1.408 −1.182 −11.780 1.00 9.17 ATOM 1219 N LEU A 290 0.442 0.111 −13.317 1.00 6.50 ATOM 1220 CA LEU A 290 −0.919 0.006 −12.767 1.00 7.32 ATOM 1221 CB LEU A 290 −1.058 0.932 −11.529 1.00 9.11 ATOM 1222 CG LEU A 290 −0.895 2.437 −11.810 1.00 9.49 ATOM 1223 CD1 LEU A 290 −2.127 3.091 −12.475 1.00 11.92 ATOM 1224 CD2 LEU A 290 −0.569 3.260 −10.567 1.00 11.15 ATOM 1225 C LEU A 290 −1.911 0.306 −13.848 1.00 8.73 ATOM 1226 O LEU A 290 −1.551 0.899 −14.911 1.00 8.82 ATOM 1227 N GLY A 291 −3.128 −0.080 −13.637 1.00 8.02 ATOM 1228 CA GLY A 291 −4.174 0.294 −14.574 1.00 10.38 ATOM 1229 C GLY A 291 −5.466 −0.425 −14.364 1.00 10.59 ATOM 1230 O GLY A 291 −5.650 −1.168 −13.389 1.00 12.54 ATOM 1231 N GLY A 292 −6.365 −0.153 −15.267 1.00 12.32 ATOM 1232 CA GLY A 292 −7.724 −0.730 −15.196 1.00 14.27 ATOM 1233 C GLY A 292 −8.348 −0.611 −16.561 1.00 15.55 ATOM 1234 O GLY A 292 −7.722 −0.212 −17.508 1.00 12.08 ATOM 1235 N SER A 293 −9.592 −1.090 −16.694 1.00 16.07 ATOM 1236 CA SER A 293 −10.264 −1.018 −17.954 1.00 15.01 ATOM 1237 CB SER A 293 −11.111 −2.264 −18.162 1.00 19.77 ATOM 1238 OG SER A 293 −12.008 −2.313 −17.128 1.00 22.56 ATOM 1239 C SER A 293 −11.052 0.254 −18.114 1.00 12.44 ATOM 1240 O SER A 293 −11.523 0.865 −17.169 1.00 15.68 ATOM 1241 N GLY A 294 −11.138 0.665 −19.382 1.00 10.69 ATOM 1242 CA GLY A 294 −11.955 1.805 −19.766 1.00 11.92 ATOM 1243 C GLY A 294 −11.173 3.008 −20.208 1.00 11.73 ATOM 1244 O GLY A 294 −9.941 2.965 −20.296 1.00 10.64 ATOM 1245 N SER A 295 −11.847 4.046 −20.606 1.00 11.59 ATOM 1246 CA SER A 295 −11.242 5.215 −21.123 1.00 11.60 ATOM 1247 CB SER A 295 −12.337 6.112 −21.711 1.00 14.59 ATOM 1248 OG SER A 295 −13.251 6.441 −20.755 1.00 17.83 ATOM 1249 C SER A 295 −10.405 5.956 −20.041 1.00 11.02 ATOM 1250 O SER A 295 −10.576 5.752 −18.868 1.00 12.16 ATOM 1251 N CYS A 296 −9.461 6.753 −20.539 1.00 9.96 ATOM 1252 CA CYS A 296 −8.648 7.626 −19.719 1.00 9.95 ATOM 1253 CB CYS A 296 −7.531 8.234 −20.547 1.00 9.38 ATOM 1254 SG CYS A 296 −6.451 9.352 −19.620 1.00 10.13 ATOM 1255 C CYS A 296 −9.567 8.755 −19.167 1.00 10.16 ATOM 1256 O CYS A 296 −10.116 9.498 −19.984 1.00 11.87 ATOM 1257 N SER A 297 −9.661 8.912 −17.872 1.00 9.61 ATOM 1258 CA SER A 297 −10.508 9.933 −17.412 1.00 11.76 ATOM 1259 CB SER A 297 −10.893 9.683 −15.989 1.00 16.12 ATOM 1260 OG SER A 297 −9.836 10.089 −15.117 1.00 16.26 ATOM 1261 C SER A 297 −9.932 11.317 −17.506 1.00 10.23 ATOM 1262 O SER A 297 −8.671 11.474 −17.572 1.00 9.33 ATOM 1263 N SER A 298 −10.749 12.339 −17.477 1.00 10.50 ATOM 1264 CA SER A 298 −10.279 13.700 −17.536 1.00 10.32 ATOM 1265 CB SER A 298 −11.339 14.792 −17.490 1.00 13.80 ATOM 1266 OG SER A 298 −12.265 14.461 −16.607 1.00 22.94 ATOM 1267 C SER A 298 −9.382 13.973 −16.289 1.00 9.57 ATOM 1268 O SER A 298 −8.435 14.706 −16.465 1.00 9.26 ATOM 1269 N THR A 299 −9.664 13.438 −15.111 1.00 9.37 ATOM 1270 CA THR A 299 −8.859 13.684 −13.924 1.00 9.40 ATOM 1271 CB THR A 299 −9.462 12.907 −12.745 1.00 11.36 ATOM 1272 OG1 THR A 299 −10.792 13.425 −12.515 1.00 14.11 ATOM 1273 CG2 THR A 299 −8.661 13.064 −11.448 1.00 12.52 ATOM 1274 C THR A 299 −7.435 13.166 −14.230 1.00 8.25 ATOM 1275 O THR A 299 −6.464 13.828 −13.910 1.00 8.51 ATOM 1276 N THR A 300 −7.329 11.968 −14.800 1.00 7.52 ATOM 1277 CA THR A 300 −6.016 11.418 −15.103 1.00 6.60 ATOM 1278 CB THR A 300 −6.137 9.945 −15.481 1.00 6.73 ATOM 1279 OG1 THR A 300 −6.888 9.287 −14.435 1.00 7.87 ATOM 1280 CG2 THR A 300 −4.814 9.305 −15.645 1.00 7.35 ATOM 1281 C THR A 300 −5.279 12.204 −16.188 1.00 6.06 ATOM 1282 O THR A 300 −4.110 12.514 −16.038 1.00 5.90 ATOM 1283 N GLN A 301 −5.944 12.526 −17.287 1.00 5.83 ATOM 1284 CA GLN A 301 −5.328 13.260 −18.364 1.00 5.71 ATOM 1285 CB GLN A 301 −6.272 13.397 −19.540 1.00 5.57 ATOM 1286 CG GLN A 301 −5.614 14.030 −20.753 1.00 6.20 ATOM 1287 CD GLN A 301 −4.531 13.131 −21.341 1.00 6.83 ATOM 1288 OE1 GLN A 301 −4.846 12.017 −21.877 1.00 8.22 ATOM 1289 NE2 GLN A 301 −3.303 13.510 −21.284 1.00 6.95 ATOM 1290 C GLN A 301 −4.884 14.624 −17.865 1.00 5.60 ATOM 1291 O GLN A 301 −3.794 15.072 −18.220 1.00 5.56 ATOM 1292 N ASN A 302 −5.677 15.259 −17.046 1.00 5.70 ATOM 1293 CA ASN A 302 −5.264 16.565 −16.478 1.00 6.86 ATOM 1294 CB ASN A 302 −6.387 17.163 −15.567 1.00 7.92 ATOM 1295 CG ASN A 302 −7.558 17.723 −16.299 1.00 10.64 ATOM 1296 OD1 ASN A 302 −7.501 17.998 −17.461 1.00 12.43 ATOM 1297 ND2 ASN A 302 −8.627 17.798 −15.595 1.00 11.71 ATOM 1298 C ASN A 302 −3.960 16.483 −15.708 1.00 5.90 ATOM 1299 O ASN A 302 −3.064 17.313 −15.787 1.00 6.09 ATOM 1300 N ALA A 303 −3.887 15.420 −14.890 1.00 5.76 ATOM 1301 CA ALA A 303 −2.657 15.183 −14.093 1.00 5.36 ATOM 1302 CB ALA A 303 −2.896 14.077 −13.107 1.00 5.81 ATOM 1303 C ALA A 303 −1.428 14.942 −14.973 1.00 4.93 ATOM 1304 O ALA A 303 −0.344 15.487 −14.730 1.00 5.87 ATOM 1305 N ILE A 304 −1.590 14.069 −15.990 1.00 4.83 ATOM 1306 CA ILE A 304 −0.550 13.806 −16.933 1.00 5.22 ATOM 1307 CB ILE A 304 −0.994 12.740 −17.912 1.00 5.57 ATOM 1308 CG1 ILE A 304 −1.111 11.406 −17.157 1.00 6.10 ATOM 1309 CD1 ILE A 304 −1.828 10.367 −17.995 1.00 8.37 ATOM 1310 CG2 ILE A 304 −0.095 12.628 −19.142 1.00 5.77 ATOM 1311 C ILE A 304 −0.099 15.100 −17.634 1.00 5.29 ATOM 1312 O ILE A 304 1.084 15.298 −17.837 1.00 5.61 ATOM 1313 N ASN A 305 −1.087 15.923 −18.047 1.00 5.61 ATOM 1314 CA ASN A 305 −0.716 17.178 −18.713 1.00 5.77 ATOM 1315 CB ASN A 305 −1.967 17.881 −19.159 1.00 6.64 ATOM 1316 CG ASN A 305 −2.768 17.202 −20.272 1.00 6.95 ATOM 1317 OD1 ASN A 305 −2.271 16.312 −20.956 1.00 9.05 ATOM 1318 ND2 ASN A 305 −3.996 17.672 −20.462 1.00 8.02 ATOM 1319 C ASN A 305 0.134 18.062 −17.809 1.00 6.37 ATOM 1320 O ASN A 305 1.065 18.651 −18.282 1.00 6.68 ATOM 1321 N THR A 306 −0.255 18.131 −16.562 1.00 5.52 ATOM 1322 CA THR A 306 0.506 18.859 −15.564 1.00 6.89 ATOM 1323 CB THR A 306 −0.171 18.851 −14.208 1.00 8.46 ATOM 1324 OG1 THR A 306 −1.363 19.694 −14.327 1.00 13.62 ATOM 1325 CG2 THR A 306 0.677 19.375 −13.089 1.00 9.84 ATOM 1326 C THR A 306 1.927 18.309 −15.431 1.00 6.74 ATOM 1327 O THR A 306 2.940 19.039 −15.483 1.00 6.54 ATOM 1328 N ALA A 307 2.039 16.969 −15.269 1.00 5.72 ATOM 1329 CA ALA A 307 3.397 16.378 −15.135 1.00 5.35 ATOM 1330 CB ALA A 307 3.250 14.875 −14.941 1.00 6.32 ATOM 1331 C ALA A 307 4.266 16.693 −16.339 1.00 5.04 ATOM 1332 O ALA A 307 5.444 17.110 −16.226 1.00 5.83 ATOM 1333 N ARG A 308 3.708 16.497 −17.551 1.00 5.47 ATOM 1334 CA ARG A 308 4.461 16.794 −18.795 1.00 5.90 ATOM 1335 CB ARG A 308 3.599 16.473 −20.010 1.00 6.22 ATOM 1336 CG ARG A 308 3.317 14.979 −20.183 1.00 6.61 ATOM 1337 CD ARG A 308 2.259 14.780 −21.218 1.00 7.12 ATOM 1338 NE ARG A 308 2.456 15.493 −22.461 1.00 8.07 ATOM 1339 CZ ARG A 308 3.367 15.207 −23.357 1.00 8.69 ATOM 1340 NH1 ARG A 308 4.056 14.111 −23.272 1.00 8.42 ATOM 1341 NH2 ARG A 308 3.684 16.030 −24.339 1.00 10.95 ATOM 1342 C ARG A 308 4.896 18.249 −18.874 1.00 6.20 ATOM 1343 O ARG A 308 6.011 18.559 −19.218 1.00 7.50 ATOM 1344 N SER A 309 4.010 19.135 −18.400 1.00 6.49 ATOM 1345 CA SER A 309 4.311 20.632 −18.474 1.00 7.46 ATOM 1346 CB SER A 309 3.065 21.377 −18.074 1.00 9.00 ATOM 1347 OG SER A 309 2.768 21.369 −16.677 1.00 12.74 ATOM 1348 C SER A 309 5.418 20.953 −17.474 1.00 7.02 ATOM 1349 O SER A 309 6.176 21.940 −17.651 1.00 8.86 ATOM 1350 N ASN A 310 5.570 20.149 −16.413 1.00 6.46 ATOM 1351 CA ASN A 310 6.658 20.310 −15.445 1.00 7.47 ATOM 1352 CB ASN A 310 6.181 19.813 −14.090 1.00 8.11 ATOM 1353 CG ASN A 310 5.201 20.717 −13.445 1.00 9.14 ATOM 1354 OD1 ASN A 310 5.203 21.951 −13.765 1.00 11.68 ATOM 1355 ND2 ASN A 310 4.404 20.234 −12.539 1.00 8.66 ATOM 1356 C ASN A 310 7.907 19.599 −15.889 1.00 7.63 ATOM 1357 O ASN A 310 8.902 19.602 −15.152 1.00 10.73 ATOM 1358 N GLY A 311 7.941 18.968 −17.074 1.00 7.13 ATOM 1359 CA GLY A 311 9.152 18.388 −17.626 1.00 7.00 ATOM 1360 C GLY A 311 9.249 16.892 −17.370 1.00 6.93 ATOM 1361 O GLY A 311 10.332 16.301 −17.617 1.00 10.07 ATOM 1362 N THR A 312 8.223 16.272 −16.805 1.00 6.21 ATOM 1363 CA THR A 312 8.311 14.833 −16.390 1.00 5.71 ATOM 1364 CB THR A 312 7.471 14.645 −15.144 1.00 6.01 ATOM 1365 OG1 THR A 312 7.915 15.560 −14.115 1.00 7.73 ATOM 1366 CG2 THR A 312 7.541 13.221 −14.606 1.00 6.28 ATOM 1367 C THR A 312 7.733 13.959 −17.484 1.00 5.44 ATOM 1368 O THR A 312 6.570 14.161 −17.948 1.00 6.74 ATOM 1369 N VAL A 313 8.495 12.967 −17.960 1.00 4.97 ATOM 1370 CA VAL A 313 8.008 11.932 −18.874 1.00 5.06 ATOM 1371 CB VAL A 313 9.149 11.392 −19.742 1.00 5.61 ATOM 1372 CG1 VAL A 313 10.143 10.576 −18.946 1.00 5.33 ATOM 1373 CG2 VAL A 313 8.621 10.560 −20.931 1.00 6.27 ATOM 1374 C VAL A 313 7.262 10.886 −18.085 1.00 4.65 ATOM 1375 O VAL A 313 7.659 10.524 −16.971 1.00 5.58 ATOM 1376 N VAL A 314 6.198 10.368 −18.679 1.00 4.67 ATOM 1377 CA VAL A 314 5.283 9.436 −17.993 1.00 4.36 ATOM 1378 CB VAL A 314 3.871 10.027 −17.928 1.00 5.26 ATOM 1379 CG1 VAL A 314 2.969 9.092 −17.155 1.00 6.38 ATOM 1380 CG2 VAL A 314 3.888 11.375 −17.243 1.00 5.78 ATOM 1381 C VAL A 314 5.308 8.083 −18.713 1.00 4.50 ATOM 1382 O VAL A 314 4.913 8.041 −19.878 1.00 5.54 ATOM 1383 N VAL A 315 5.723 7.034 −18.026 1.00 4.48 ATOM 1384 CA VAL A 315 5.864 5.697 −18.561 1.00 4.66 ATOM 1385 CB VAL A 315 7.331 5.209 −18.333 1.00 5.25 ATOM 1386 CG1 VAL A 315 7.495 3.800 −18.906 1.00 5.92 ATOM 1387 CG2 VAL A 315 8.331 6.172 −19.014 1.00 5.37 ATOM 1388 C VAL A 315 4.879 4.801 −17.885 1.00 4.81 ATOM 1389 O VAL A 315 4.806 4.761 −16.667 1.00 5.27 ATOM 1390 N ILE A 316 4.114 4.029 −18.694 1.00 4.90 ATOM 1391 CA ILE A 316 2.950 3.299 −18.197 1.00 4.70 ATOM 1392 CB ILE A 316 1.640 4.016 −18.580 1.00 5.52 ATOM 1393 CG1 ILE A 316 1.716 5.459 −18.054 1.00 6.42 ATOM 1394 CD1 ILE A 316 0.497 6.304 −18.292 1.00 8.35 ATOM 1395 CG2 ILE A 316 0.449 3.256 −18.009 1.00 6.28 ATOM 1396 C ILE A 316 2.925 1.882 −18.763 1.00 4.57 ATOM 1397 O ILE A 316 3.269 1.623 −19.921 1.00 5.52 ATOM 1398 N ALA A 317 2.570 0.936 −17.888 1.00 3.86 ATOM 1399 CA ALA A 317 2.272 −0.437 −18.297 1.00 4.33 ATOM 1400 CB ALA A 317 2.171 −1.307 −17.094 1.00 4.61 ATOM 1401 C ALA A 317 0.986 −0.541 −19.071 1.00 5.00 ATOM 1402 O ALA A 317 −0.059 −0.077 −18.632 1.00 6.34 ATOM 1403 N ALA A 318 1.023 −1.256 −20.211 1.00 4.73 ATOM 1404 CA ALA A 318 −0.216 −1.305 −21.106 1.00 5.48 ATOM 1405 CB ALA A 318 0.198 −1.737 −22.482 1.00 6.17 ATOM 1406 C ALA A 318 −1.235 −2.202 −20.593 1.00 5.66 ATOM 1407 O ALA A 318 −2.423 −2.128 −21.118 1.00 6.82 ATOM 1408 N GLY A 319 −0.996 −3.077 −19.640 1.00 5.02 ATOM 1409 CA GLY A 319 −1.978 −4.025 −19.097 1.00 5.57 ATOM 1410 C GLY A 319 −1.810 −5.445 −19.604 1.00 5.40 ATOM 1411 O GLY A 319 −1.131 −5.710 −20.563 1.00 5.78 ATOM 1412 N ASN A 320 −2.476 −6.372 −18.936 1.00 6.68 ATOM 1413 CA ASN A 320 −2.191 −7.812 −18.983 1.00 7.71 ATOM 1414 CB ASN A 320 −1.642 −8.326 −17.698 1.00 8.60 ATOM 1415 CG ASN A 320 −0.449 −7.538 −17.205 1.00 10.07 ATOM 1416 OD1 ASN A 320 0.421 −7.027 −17.972 1.00 8.35 ATOM 1417 ND2 ASN A 320 −0.340 −7.442 −15.900 1.00 13.88 ATOM 1418 C ASN A 320 −3.426 −8.618 −19.368 1.00 8.66 ATOM 1419 O ASN A 320 −3.589 −9.739 −18.876 1.00 10.16 ATOM 1420 N ASP A 321 −4.238 −8.089 −20.231 1.00 7.49 ATOM 1421 CA ASP A 321 −5.531 −8.706 −20.616 1.00 9.71 ATOM 1422 CB ASP A 321 −6.569 −7.538 −20.641 1.00 11.83 ATOM 1423 CG ASP A 321 −6.378 −6.550 −19.371 1.00 15.03 ATOM 1424 OD1 ASP A 321 −7.039 −6.989 −18.395 1.00 16.94 ATOM 1425 OD2 ASP A 321 −5.644 −5.424 −19.304 1.00 16.25 ATOM 1426 C ASP A 321 −5.435 −9.406 −21.926 1.00 8.14 ATOM 1427 O ASP A 321 −6.492 −9.867 −22.471 1.00 9.13 ATOM 1428 N ASN A 322 −4.316 −9.500 −22.626 1.00 6.51 ATOM 1429 CA ASN A 322 −4.212 −10.052 −23.936 1.00 6.61 ATOM 1430 CB ASN A 322 −4.312 −11.592 −23.964 1.00 7.14 ATOM 1431 CG ASN A 322 −4.082 −12.168 −25.290 1.00 7.15 ATOM 1432 OD1 ASN A 322 −3.359 −11.705 −26.143 1.00 8.73 ATOM 1433 ND2 ASN A 322 −4.772 −13.340 −25.552 1.00 8.38 ATOM 1434 C ASN A 322 −5.302 −9.416 −24.786 1.00 7.81 ATOM 1435 O ASN A 322 −5.985 −10.144 −25.583 1.00 8.91 ATOM 1436 N ASP A 323 −5.384 −8.091 −24.774 1.00 7.92 ATOM 1437 CA ASP A 323 −6.486 −7.430 −25.450 1.00 9.18 ATOM 1438 CB ASP A 323 −7.615 −7.221 −24.435 1.00 9.74 ATOM 1439 CG ASP A 323 −9.003 −6.817 −25.038 1.00 12.12 ATOM 1440 OD1 ASP A 323 −9.199 −6.968 −26.214 1.00 13.77 ATOM 1441 OD2 ASP A 323 −9.741 −6.391 −24.141 1.00 16.14 ATOM 1442 C ASP A 323 −5.954 −6.124 −26.007 1.00 9.00 ATOM 1443 O ASP A 323 −4.826 −5.689 −25.817 1.00 8.00 ATOM 1444 N ASN A 324 −6.833 −5.409 −26.686 1.00 9.69 ATOM 1445 CA ASN A 324 −6.516 −4.107 −27.317 1.00 9.55 ATOM 1446 CB ASN A 324 −7.718 −3.692 −28.195 1.00 11.03 ATOM 1447 CG ASN A 324 −7.567 −2.404 −28.942 1.00 14.91 ATOM 1448 OD1 ASN A 324 −6.586 −1.707 −28.829 1.00 12.45 ATOM 1449 ND2 ASN A 324 −8.596 −2.105 −29.762 1.00 17.83 ATOM 1450 C ASN A 324 −6.290 −3.045 −26.281 1.00 8.72 ATOM 1451 O ASN A 324 −7.102 −2.666 −25.433 1.00 9.67 ATOM 1452 N SER A 325 −5.085 −2.481 −26.295 1.00 7.76 ATOM 1453 CA SER A 325 −4.607 −1.492 −25.358 1.00 8.88 ATOM 1454 CB SER A 325 −3.106 −1.303 −25.592 1.00 9.29 ATOM 1455 OG SER A 325 −2.837 −0.855 −26.892 1.00 10.13 ATOM 1456 C SER A 325 −5.345 −0.170 −25.430 1.00 9.62 ATOM 1457 O SER A 325 −5.275 0.578 −24.498 1.00 10.18 ATOM 1458 N ALA A 326 −6.131 0.027 −26.489 1.00 9.81 ATOM 1459 CA ALA A 326 −7.021 1.216 −26.567 1.00 9.49 ATOM 1460 CB ALA A 326 −7.857 1.151 −27.853 1.00 11.34 ATOM 1461 C ALA A 326 −7.946 1.287 −25.430 1.00 9.35 ATOM 1462 O ALA A 326 −8.411 2.359 −25.039 1.00 9.59 ATOM 1463 N ASN A 327 −8.277 0.161 −24.792 1.00 9.32 ATOM 1464 CA ASN A 327 −9.306 0.114 −23.811 1.00 10.59 ATOM 1465 CB ASN A 327 −10.310 −0.955 −24.250 1.00 14.30 ATOM 1466 CG ASN A 327 −10.933 −0.581 −25.618 1.00 17.57 ATOM 1467 OD1 ASN A 327 −11.244 0.620 −25.877 1.00 20.41 ATOM 1468 ND2 ASN A 327 −11.070 −1.541 −26.436 1.00 21.91 ATOM 1469 C ASN A 327 −8.919 −0.099 −22.385 1.00 9.65 ATOM 1470 O ASN A 327 −9.694 −0.446 −21.519 1.00 9.83 ATOM 1471 N TYR A 328 −7.666 0.272 −22.093 1.00 7.86 ATOM 1472 CA TYR A 328 −7.044 0.097 −20.811 1.00 7.81 ATOM 1473 CB TYR A 328 −6.128 −1.134 −20.761 1.00 9.19 ATOM 1474 CG TYR A 328 −6.908 −2.396 −20.972 1.00 9.72 ATOM 1475 CD1 TYR A 328 −7.608 −2.989 −19.926 1.00 11.80 ATOM 1476 CE1 TYR A 328 −8.431 −4.102 −20.184 1.00 12.69 ATOM 1477 CZ TYR A 328 −8.561 −4.560 −21.406 1.00 11.69 ATOM 1478 OH TYR A 328 −9.343 −5.726 −21.605 1.00 14.32 ATOM 1479 CE2 TYR A 328 −7.984 −3.990 −22.483 1.00 11.45 ATOM 1480 CD2 TYR A 328 −7.148 −2.886 −22.214 1.00 10.35 ATOM 1481 C TYR A 328 −6.290 1.396 −20.433 1.00 7.90 ATOM 1482 O TYR A 328 −5.498 1.895 −21.218 1.00 7.66 ATOM 1483 N ASN A 329 −6.609 1.899 −19.270 1.00 8.30 ATOM 1484 CA ASN A 329 −6.059 3.156 −18.757 1.00 8.15 ATOM 1485 CB ASN A 329 −7.231 4.096 −18.249 1.00 8.84 ATOM 1486 CG ASN A 329 −7.991 3.505 −17.140 1.00 12.04 ATOM 1487 OD1 ASN A 329 −7.583 3.534 −16.014 1.00 14.73 ATOM 1488 ND2 ASN A 329 −9.070 2.904 −17.451 1.00 12.11 ATOM 1489 C ASN A 329 −5.044 2.889 −17.649 1.00 8.51 ATOM 1490 O ASN A 329 −5.195 1.845 −16.958 1.00 10.32 ATOM 1491 N PRO A 330 −4.121 3.756 −17.334 1.00 8.01 ATOM 1492 CA PRO A 330 −3.885 5.035 −18.023 1.00 7.20 ATOM 1493 CB PRO A 330 −3.242 5.881 −16.928 1.00 7.49 ATOM 1494 CG PRO A 330 −2.477 4.873 −16.070 1.00 8.85 ATOM 1495 CD PRO A 330 −3.350 3.633 −16.077 1.00 9.41 ATOM 1496 C PRO A 330 −2.998 4.915 −19.247 1.00 6.48 ATOM 1497 O PRO A 330 −2.652 5.928 −19.852 1.00 6.33 ATOM 1498 N GLY A 331 −2.705 3.706 −19.732 1.00 6.43 ATOM 1499 CA GLY A 331 −1.905 3.608 −20.881 1.00 7.08 ATOM 1500 C GLY A 331 −2.476 4.199 −22.150 1.00 7.24 ATOM 1501 O GLY A 331 −1.780 4.590 −23.070 1.00 8.74 ATOM 1502 N ASN A 332 −3.824 4.242 −22.211 1.00 7.21 ATOM 1503 CA ASN A 332 −4.584 4.885 −23.279 1.00 6.98 ATOM 1504 CB ASN A 332 −5.918 4.176 −23.473 1.00 6.72 ATOM 1505 CG ASN A 332 −6.839 4.336 −22.307 1.00 6.75 ATOM 1506 OD1 ASN A 332 −6.509 4.970 −21.310 1.00 7.09 ATOM 1507 ND2 ASN A 332 −8.046 3.759 −22.400 1.00 7.72 ATOM 1508 C ASN A 332 −4.725 6.366 −23.124 1.00 7.68 ATOM 1509 O ASN A 332 −5.337 7.000 −24.009 1.00 9.61 ATOM 1510 N CYS A 333 −4.098 6.973 −22.131 1.00 6.78 ATOM 1511 CA CYS A 333 −4.102 8.444 −22.043 1.00 6.97 ATOM 1512 CB CYS A 333 −3.637 8.849 −20.688 1.00 6.66 ATOM 1513 SG CYS A 333 −4.699 8.310 −19.333 1.00 8.62 ATOM 1514 C CYS A 333 −3.163 8.966 −23.106 1.00 6.49 ATOM 1515 O CYS A 333 −2.347 8.283 −23.739 1.00 7.78 ATOM 1516 N ASN A 334 −3.266 10.264 −23.440 1.00 6.48 ATOM 1517 CA ASN A 334 −2.400 10.935 −24.363 1.00 6.14 ATOM 1518 CB ASN A 334 −3.094 12.027 −25.154 1.00 7.05 ATOM 1519 CG ASN A 334 −4.204 11.493 −26.001 1.00 9.21 ATOM 1520 OD1 ASN A 334 −3.985 10.511 −26.724 1.00 12.82 ATOM 1521 ND2 ASN A 334 −5.355 12.021 −25.894 1.00 10.07 ATOM 1522 C ASN A 334 −1.150 11.459 −23.666 1.00 5.19 ATOM 1523 O ASN A 334 −1.198 11.821 −22.487 1.00 6.15 ATOM 1524 N GLY A 335 −0.060 11.529 −24.412 1.00 4.99 ATOM 1525 CA GLY A 335 1.206 12.057 −23.884 1.00 5.47 ATOM 1526 C GLY A 335 1.968 11.159 −22.959 1.00 6.11 ATOM 1527 O GLY A 335 2.698 11.671 −22.114 1.00 6.82 ATOM 1528 N VAL A 336 1.829 9.839 −23.139 1.00 5.58 ATOM 1529 CA VAL A 336 2.495 8.867 −22.287 1.00 5.14 ATOM 1530 CB VAL A 336 1.476 8.112 −21.388 1.00 5.83 ATOM 1531 CG1 VAL A 336 0.588 9.068 −20.579 1.00 6.64 ATOM 1532 CG2 VAL A 336 0.593 7.162 −22.146 1.00 7.05 ATOM 1533 C VAL A 336 3.267 7.923 −23.121 1.00 5.06 ATOM 1534 O VAL A 336 2.993 7.730 −24.311 1.00 5.59 ATOM 1535 N VAL A 337 4.219 7.239 −22.493 1.00 4.68 ATOM 1536 CA VAL A 337 4.959 6.131 −23.132 1.00 5.01 ATOM 1537 CB VAL A 337 6.438 6.150 −22.796 1.00 5.68 ATOM 1538 CG1 VAL A 337 7.170 5.087 −23.515 1.00 6.36 ATOM 1539 CG2 VAL A 337 7.054 7.547 −23.050 1.00 6.32 ATOM 1540 C VAL A 337 4.308 4.836 −22.657 1.00 4.74 ATOM 1541 O VAL A 337 4.492 4.438 −21.489 1.00 4.49 ATOM 1542 N ASN A 338 3.515 4.207 −23.512 1.00 4.54 ATOM 1543 CA ASN A 338 2.754 3.016 −23.149 1.00 4.70 ATOM 1544 CB ASN A 338 1.361 3.031 −23.774 1.00 5.18 ATOM 1545 CG ASN A 338 0.442 2.043 −23.130 1.00 6.18 ATOM 1546 OD1 ASN A 338 0.572 1.674 −22.000 1.00 6.42 ATOM 1547 ND2 ASN A 338 −0.597 1.626 −23.907 1.00 7.16 ATOM 1548 C ASN A 338 3.517 1.799 −23.608 1.00 4.85 ATOM 1549 O ASN A 338 3.987 1.717 −24.771 1.00 5.38 ATOM 1550 N VAL A 339 3.701 0.865 −22.672 1.00 4.31 ATOM 1551 CA VAL A 339 4.721 −0.207 −22.825 1.00 4.83 ATOM 1552 CB VAL A 339 5.724 −0.114 −21.673 1.00 4.41 ATOM 1553 CG1 VAL A 339 6.798 −1.201 −21.814 1.00 4.27 ATOM 1554 CG2 VAL A 339 6.369 1.265 −21.631 1.00 4.86 ATOM 1555 C VAL A 339 4.066 −1.580 −22.810 1.00 4.15 ATOM 1556 O VAL A 339 3.390 −1.965 −21.847 1.00 5.14 ATOM 1557 N GLY A 340 4.309 −2.323 −23.918 1.00 4.14 ATOM 1558 CA GLY A 340 3.946 −3.754 −23.984 1.00 4.32 ATOM 1559 C GLY A 340 5.086 −4.592 −23.475 1.00 5.09 ATOM 1560 O GLY A 340 6.233 −4.182 −23.361 1.00 5.27 ATOM 1561 N SER A 341 4.789 −5.865 −23.154 1.00 4.87 ATOM 1562 CA SER A 341 5.745 −6.772 −22.570 1.00 4.72 ATOM 1563 CB SER A 341 5.134 −7.431 −21.362 1.00 5.28 ATOM 1564 OG SER A 341 6.007 −8.451 −20.867 1.00 5.47 ATOM 1565 C SER A 341 6.183 −7.864 −23.510 1.00 4.95 ATOM 1566 O SER A 341 5.275 −8.491 −24.153 1.00 5.74 ATOM 1567 N VAL A 342 7.463 −8.123 −23.632 1.00 4.86 ATOM 1568 CA VAL A 342 8.007 −9.324 −24.246 1.00 4.81 ATOM 1569 CB VAL A 342 9.097 −9.062 −25.282 1.00 5.65 ATOM 1570 CG1 VAL A 342 8.540 −8.234 −26.457 1.00 6.42 ATOM 1571 CG2 VAL A 342 10.325 −8.396 −24.683 1.00 5.90 ATOM 1572 C VAL A 342 8.475 −10.276 −23.214 1.00 5.27 ATOM 1573 O VAL A 342 8.895 −9.939 −22.086 1.00 5.26 ATOM 1574 N GLY A 343 8.481 −11.554 −23.586 1.00 5.24 ATOM 1575 CA GLY A 343 9.146 −12.606 −22.776 1.00 5.76 ATOM 1576 C GLY A 343 10.573 −12.750 −23.153 1.00 5.22 ATOM 1577 O GLY A 343 11.144 −12.075 −24.002 1.00 5.70 ATOM 1578 N ARG A 344 11.204 −13.785 −22.577 1.00 5.72 ATOM 1579 CA ARG A 344 12.584 −14.124 −22.810 1.00 6.92 ATOM 1580 CB ARG A 344 12.983 −15.203 −21.776 1.00 6.66 ATOM 1581 CG ARG A 344 14.487 −15.473 −21.757 1.00 7.10 ATOM 1582 CD ARG A 344 14.804 −16.311 −20.525 1.00 8.76 ATOM 1583 NE ARG A 344 16.241 −16.486 −20.323 1.00 9.16 ATOM 1584 CZ ARG A 344 16.700 −17.083 −19.207 1.00 11.98 ATOM 1585 NH1 ARG A 344 15.878 −17.579 −18.309 1.00 12.37 ATOM 1586 NH2 ARG A 344 17.984 −17.008 −18.972 1.00 14.02 ATOM 1587 C ARG A 344 12.881 −14.510 −24.255 1.00 6.39 ATOM 1588 O ARG A 344 14.015 −14.419 −24.677 1.00 7.27 ATOM 1589 N ASN A 345 11.867 −14.912 −25.008 1.00 6.91 ATOM 1590 CA ASN A 345 12.026 −15.143 −26.417 1.00 7.10 ATOM 1591 CB ASN A 345 10.923 −16.094 −27.005 1.00 8.01 ATOM 1592 CG ASN A 345 9.509 −15.493 −26.923 1.00 8.93 ATOM 1593 OD1 ASN A 345 8.597 −15.938 −27.694 1.00 14.51 ATOM 1594 ND2 ASN A 345 9.305 −14.485 −26.190 1.00 6.88 ATOM 1595 C ASN A 345 12.074 −13.886 −27.277 1.00 7.36 ATOM 1596 O ASN A 345 12.341 −13.961 −28.459 1.00 7.72 ATOM 1597 N GLY A 346 11.806 −12.700 −26.691 1.00 6.37 ATOM 1598 CA GLY A 346 11.714 −11.502 −27.478 1.00 6.55 ATOM 1599 C GLY A 346 10.430 −11.363 −28.298 1.00 6.47 ATOM 1600 O GLY A 346 10.412 −10.466 −29.170 1.00 8.14 ATOM 1601 N GLY A 347 9.454 −12.147 −28.015 1.00 6.60 ATOM 1602 CA GLY A 347 8.131 −12.002 −28.584 1.00 6.68 ATOM 1603 C GLY A 347 7.151 −11.565 −27.560 1.00 6.36 ATOM 1604 O GLY A 347 7.410 −11.609 −26.356 1.00 6.30 ATOM 1605 N ARG A 348 5.921 −11.261 −28.009 1.00 6.37 ATOM 1606 CA ARG A 348 4.936 −10.747 −27.092 1.00 5.68 ATOM 1607 CB ARG A 348 3.579 −10.458 −27.811 1.00 6.39 ATOM 1608 CG ARG A 348 2.643 −9.668 −26.939 1.00 6.99 ATOM 1609 CD ARG A 348 1.437 −9.176 −27.719 1.00 7.49 ATOM 1610 NE ARG A 348 0.618 −10.240 −28.281 1.00 7.26 ATOM 1611 CZ ARG A 348 −0.299 −10.954 −27.678 1.00 8.60 ATOM 1612 NH1 ARG A 348 −0.680 −10.751 −26.466 1.00 8.08 ATOM 1613 NH2 ARG A 348 −0.985 −11.861 −28.441 1.00 12.89 ATOM 1614 C ARG A 348 4.700 −11.677 −25.949 1.00 5.83 ATOM 1615 O ARG A 348 4.432 −12.890 −26.126 1.00 8.22 ATOM 1616 N ALA A 349 4.730 −11.238 −24.714 1.00 5.39 ATOM 1617 CA ALA A 349 4.322 −12.074 −23.592 1.00 5.89 ATOM 1618 CB ALA A 349 4.628 −11.326 −22.268 1.00 5.69 ATOM 1619 C ALA A 349 2.875 −12.383 −23.725 1.00 6.58 ATOM 1620 O ALA A 349 2.081 −11.538 −24.097 1.00 7.04 ATOM 1621 N TYR A 350 2.476 −13.620 −23.386 1.00 7.05 ATOM 1622 CA TYR A 350 1.125 −14.098 −23.715 1.00 6.47 ATOM 1623 CB TYR A 350 0.918 −15.524 −23.199 1.00 6.57 ATOM 1624 CG TYR A 350 1.144 −15.738 −21.732 1.00 6.61 ATOM 1625 CD1 TYR A 350 0.129 −15.593 −20.800 1.00 7.23 ATOM 1626 CE1 TYR A 350 0.326 −15.829 −19.472 1.00 7.00 ATOM 1627 CZ TYR A 350 1.600 −16.245 −19.012 1.00 7.54 ATOM 1628 OH TYR A 350 1.714 −16.524 −17.630 1.00 10.10 ATOM 1629 CE2 TYR A 350 2.620 −16.357 −19.917 1.00 6.84 ATOM 1630 CD2 TYR A 350 2.419 −16.104 −21.216 1.00 7.27 ATOM 1631 C TYR A 350 0.047 −13.202 −23.190 1.00 6.81 ATOM 1632 O TYR A 350 −1.035 −13.102 −23.808 1.00 8.05 ATOM 1633 N TYR A 351 0.209 −12.555 −22.043 1.00 5.85 ATOM 1634 CA TYR A 351 −0.812 −11.744 −21.414 1.00 6.56 ATOM 1635 CB TYR A 351 −0.603 −11.743 −19.855 1.00 6.50 ATOM 1636 CG TYR A 351 0.802 −11.279 −19.504 1.00 5.99 ATOM 1637 CD1 TYR A 351 1.157 −9.936 −19.582 1.00 6.08 ATOM 1638 CE1 TYR A 351 2.462 −9.538 −19.385 1.00 5.14 ATOM 1639 CZ TYR A 351 3.472 −10.487 −19.128 1.00 5.18 ATOM 1640 OH TYR A 351 4.758 −10.041 −18.946 1.00 5.21 ATOM 1641 CE2 TYR A 351 3.110 −11.788 −18.970 1.00 5.45 ATOM 1642 CD2 TYR A 351 1.838 −12.173 −19.181 1.00 5.78 ATOM 1643 C TYR A 351 −0.781 −10.275 −21.917 1.00 5.57 ATOM 1644 O TYR A 351 −1.670 −9.539 −21.504 1.00 6.66 ATOM 1645 N SER A 352 0.235 −9.869 −22.644 1.00 5.60 ATOM 1646 CA SER A 352 0.379 −8.438 −22.835 1.00 6.09 ATOM 1647 CB SER A 352 1.690 −8.199 −23.546 1.00 5.75 ATOM 1648 OG SER A 352 1.921 −6.739 −23.614 1.00 5.93 ATOM 1649 C SER A 352 −0.718 −7.894 −23.709 1.00 5.48 ATOM 1650 O SER A 352 −1.043 −8.423 −24.791 1.00 6.27 ATOM 1651 N ASN A 353 −1.241 −6.739 −23.338 1.00 5.46 ATOM 1652 CA ASN A 353 −2.035 −5.940 −24.260 1.00 5.50 ATOM 1653 CB ASN A 353 −2.743 −4.820 −23.516 1.00 6.24 ATOM 1654 CG ASN A 353 −3.818 −5.262 −22.590 1.00 6.96 ATOM 1655 OD1 ASN A 353 −4.342 −6.380 −22.661 1.00 8.00 ATOM 1656 ND2 ASN A 353 −4.173 −4.401 −21.645 1.00 6.15 ATOM 1657 C ASN A 353 −1.214 −5.471 −25.408 1.00 6.48 ATOM 1658 O ASN A 353 0.038 −5.473 −25.368 1.00 6.94 ATOM 1659 N TYR A 354 −1.906 −5.035 −26.440 1.00 6.28 ATOM 1660 CA TYR A 354 −1.262 −4.695 −27.719 1.00 6.32 ATOM 1661 CB TYR A 354 −0.929 −5.972 −28.533 1.00 8.02 ATOM 1662 CG TYR A 354 −2.113 −6.796 −28.832 1.00 10.87 ATOM 1663 CD1 TYR A 354 −3.109 −6.409 −29.810 1.00 15.69 ATOM 1664 CE1 TYR A 354 −4.329 −7.113 −29.966 1.00 19.53 ATOM 1665 CZ TYR A 354 −4.570 −8.197 −29.068 1.00 18.06 ATOM 1666 OH TYR A 354 −5.770 −8.939 −29.168 1.00 22.96 ATOM 1667 CE2 TYR A 354 −3.617 −8.632 −28.175 1.00 13.59 ATOM 1668 CD2 TYR A 354 −2.472 −7.850 −27.987 1.00 9.66 ATOM 1669 C TYR A 354 −2.205 −3.773 −28.510 1.00 6.17 ATOM 1670 O TYR A 354 −3.396 −3.803 −28.185 1.00 8.17 ATOM 1671 N GLY A 355 −1.696 −3.024 −29.434 1.00 6.53 ATOM 1672 CA GLY A 355 −2.549 −2.075 −30.199 1.00 7.00 ATOM 1673 C GLY A 355 −1.788 −0.900 −30.644 1.00 7.60 ATOM 1674 O GLY A 355 −0.564 −0.745 −30.394 1.00 7.20 ATOM 1675 N SER A 356 −2.509 0.004 −31.317 1.00 8.55 ATOM 1676 CA SER A 356 −1.895 1.157 −31.891 1.00 9.15 ATOM 1677 CB SER A 356 −2.947 1.946 −32.736 1.00 13.62 ATOM 1678 OG SER A 356 −3.589 1.071 −33.656 1.00 25.87 ATOM 1679 C SER A 356 −1.269 2.133 −30.909 1.00 7.65 ATOM 1680 O SER A 356 −0.269 2.762 −31.266 1.00 8.60 ATOM 1681 N ASN A 357 −1.766 2.199 −29.699 1.00 6.38 ATOM 1682 CA ASN A 357 −1.252 3.128 −28.763 1.00 6.67 ATOM 1683 CB ASN A 357 −2.289 3.679 −27.810 1.00 6.94 ATOM 1684 CG ASN A 357 −2.770 2.696 −26.798 1.00 7.29 ATOM 1685 OD1 ASN A 357 −2.076 1.678 −26.493 1.00 8.50 ATOM 1686 ND2 ASN A 357 −3.887 2.928 −26.174 1.00 8.77 ATOM 1687 C ASN A 357 −0.041 2.561 −27.988 1.00 6.61 ATOM 1688 O ASN A 357 0.541 3.284 −27.180 1.00 7.75 ATOM 1689 N ILE A 358 0.437 1.369 −28.283 1.00 5.48 ATOM 1690 CA ILE A 358 1.721 0.899 −27.669 1.00 5.60 ATOM 1691 CB ILE A 358 1.940 −0.596 −27.946 1.00 6.09 ATOM 1692 CG1 ILE A 358 0.793 −1.433 −27.287 1.00 6.84 ATOM 1693 CD1 ILE A 358 0.642 −1.388 −25.847 1.00 7.02 ATOM 1694 CG2 ILE A 358 3.292 −0.994 −27.375 1.00 5.81 ATOM 1695 C ILE A 358 2.795 1.692 −28.363 1.00 5.94 ATOM 1696 O ILE A 358 2.855 1.772 −29.631 1.00 6.64 ATOM 1697 N ASP A 359 3.706 2.255 −27.555 1.00 4.84 ATOM 1698 CA ASP A 359 4.833 2.963 −28.114 1.00 4.88 ATOM 1699 CB ASP A 359 5.195 4.111 −27.169 1.00 5.64 ATOM 1700 CG ASP A 359 4.098 5.139 −27.105 1.00 6.23 ATOM 1701 OD1 ASP A 359 3.725 5.699 −28.202 1.00 7.22 ATOM 1702 OD2 ASP A 359 3.534 5.456 −26.045 1.00 6.68 ATOM 1703 C ASP A 359 6.014 2.107 −28.343 1.00 5.09 ATOM 1704 O ASP A 359 6.728 2.156 −29.387 1.00 5.79 ATOM 1705 N VAL A 360 6.368 1.270 −27.340 1.00 4.94 ATOM 1706 CA VAL A 360 7.533 0.377 −27.321 1.00 5.41 ATOM 1707 CB VAL A 360 8.777 1.064 −26.687 1.00 5.10 ATOM 1708 CG1 VAL A 360 9.197 2.330 −27.454 1.00 5.67 ATOM 1709 CG2 VAL A 360 8.550 1.397 −25.221 1.00 5.78 ATOM 1710 C VAL A 360 7.208 −0.861 −26.516 1.00 4.41 ATOM 1711 O VAL A 360 6.226 −0.886 −25.782 1.00 5.19 ATOM 1712 N ALA A 361 8.065 −1.866 −26.644 1.00 4.82 ATOM 1713 CA ALA A 361 8.032 −3.025 −25.786 1.00 4.40 ATOM 1714 CB ALA A 361 7.837 −4.266 −26.606 1.00 5.13 ATOM 1715 C ALA A 361 9.257 −3.135 −24.955 1.00 4.85 ATOM 1716 O ALA A 361 10.332 −2.659 −25.348 1.00 4.85 ATOM 1717 N ALA A 362 9.157 −3.913 −23.871 1.00 4.38 ATOM 1718 CA ALA A 362 10.343 −4.211 −23.017 1.00 4.66 ATOM 1719 CB ALA A 362 10.604 −3.075 −22.040 1.00 5.12 ATOM 1720 C ALA A 362 10.063 −5.544 −22.349 1.00 4.63 ATOM 1721 O ALA A 362 8.901 −5.949 −22.243 1.00 4.56 ATOM 1722 N PRO A 363 11.098 −6.202 −21.794 1.00 4.75 ATOM 1723 CA PRO A 363 10.854 −7.457 −21.113 1.00 5.03 ATOM 1724 CB PRO A 363 12.310 −7.933 −20.807 1.00 5.56 ATOM 1725 CG PRO A 363 13.112 −6.747 −20.837 1.00 5.52 ATOM 1726 CD PRO A 363 12.537 −5.886 −21.890 1.00 5.59 ATOM 1727 C PRO A 363 10.035 −7.296 −19.882 1.00 4.95 ATOM 1728 O PRO A 363 10.410 −6.600 −18.929 1.00 6.07 ATOM 1729 N GLY A 364 8.902 −8.019 −19.811 1.00 4.45 ATOM 1730 CA GLY A 364 8.134 −8.221 −18.633 1.00 4.81 ATOM 1731 C GLY A 364 7.975 −9.622 −18.137 1.00 4.58 ATOM 1732 O GLY A 364 7.366 −9.881 −17.122 1.00 4.95 ATOM 1733 N GLY A 365 8.519 −10.551 −18.964 1.00 5.43 ATOM 1734 CA GLY A 365 8.549 −11.968 −18.574 1.00 5.30 ATOM 1735 C GLY A 365 7.305 −12.738 −18.958 1.00 5.95 ATOM 1736 O GLY A 365 6.234 −12.183 −19.175 1.00 6.71 ATOM 1737 N ALA A 366 7.496 −14.034 −19.093 1.00 6.43 ATOM 1738 CA ALA A 366 6.381 −14.950 −19.434 1.00 6.70 ATOM 1739 CB ALA A 366 6.309 −15.187 −20.901 1.00 6.91 ATOM 1740 C ALA A 366 6.565 −16.219 −18.615 1.00 6.85 ATOM 1741 O ALA A 366 7.339 −17.117 −19.085 1.00 8.17 ATOM 1742 N GLN A 367 5.892 −16.305 −17.493 1.00 6.60 ATOM 1743 CA GLN A 367 6.042 −17.389 −16.546 1.00 7.44 ATOM 1744 CB GLN A 367 5.922 −16.806 −15.131 1.00 7.93 ATOM 1745 CG GLN A 367 7.066 −15.850 −14.729 1.00 7.53 ATOM 1746 CD GLN A 367 6.836 −15.297 −13.354 1.00 7.96 ATOM 1747 OE1 GLN A 367 5.786 −14.743 −13.042 1.00 9.16 ATOM 1748 NE2 GLN A 367 7.814 −15.474 −12.451 1.00 7.49 ATOM 1749 C GLN A 367 4.943 −18.410 −16.743 1.00 7.17 ATOM 1750 O GLN A 367 3.833 −18.104 −17.204 1.00 8.46 ATOM 1751 N SER A 368 5.197 −19.643 −16.290 1.00 7.60 ATOM 1752 CA SER A 368 4.185 −20.697 −16.319 1.00 7.36 ATOM 1753 CB SER A 368 4.466 −21.784 −17.334 1.00 8.32 ATOM 1754 OG SER A 368 5.544 −22.635 −16.952 1.00 8.40 ATOM 1755 C SER A 368 3.904 −21.233 −14.948 1.00 8.67 ATOM 1756 O SER A 368 3.054 −22.130 −14.788 1.00 9.23 ATOM 1757 N PHE A 369 4.559 −20.690 −13.890 1.00 9.15 ATOM 1758 CA PHE A 369 4.333 −21.108 −12.502 1.00 10.03 ATOM 1759 CB PHE A 369 4.942 −22.468 −12.220 1.00 10.13 ATOM 1760 CG PHE A 369 6.408 −22.556 −12.371 1.00 9.92 ATOM 1761 CD1 PHE A 369 7.050 −22.825 −13.534 1.00 9.82 ATOM 1762 CE1 PHE A 369 8.369 −22.888 −13.710 1.00 10.94 ATOM 1763 CZ PHE A 369 9.155 −22.712 −12.597 1.00 11.14 ATOM 1764 CE2 PHE A 369 8.604 −22.438 −11.384 1.00 11.18 ATOM 1765 CD2 PHE A 369 7.259 −22.383 −11.204 1.00 10.58 ATOM 1766 C PHE A 369 4.873 −20.011 −11.596 1.00 10.37 ATOM 1767 O PHE A 369 5.642 −19.135 −12.064 1.00 9.55 ATOM 1768 N ALA A 370 4.498 −20.053 −10.312 1.00 14.06 ATOM 1769 CA ALA A 370 4.892 −18.969 −9.415 1.00 12.94 ATOM 1770 CB ALA A 370 4.146 −19.099 −8.073 1.00 16.83 ATOM 1771 C ALA A 370 6.391 −18.943 −9.214 1.00 10.84 ATOM 1772 O ALA A 370 7.018 −19.891 −9.012 1.00 12.18 ATOM 1773 N ASN A 371 6.922 −17.727 −9.331 1.00 10.56 ATOM 1774 CA ASN A 371 8.346 −17.495 −9.153 1.00 9.61 ATOM 1775 CB ASN A 371 8.838 −17.891 −7.682 1.00 10.27 ATOM 1776 CG ASN A 371 10.039 −17.106 −7.271 1.00 11.82 ATOM 1777 OD1 ASN A 371 10.136 −15.908 −7.525 1.00 9.95 ATOM 1778 ND2 ASN A 371 10.957 −17.732 −6.566 1.00 14.01 ATOM 1779 C ASN A 371 9.237 −18.202 −10.154 1.00 7.76 ATOM 1780 O ASN A 371 10.424 −18.349 −9.972 1.00 9.49 ATOM 1781 N ASP A 372 8.660 −18.554 −11.328 1.00 7.67 ATOM 1782 CA ASP A 372 9.439 −19.105 −12.423 1.00 7.36 ATOM 1783 CB ASP A 372 8.388 −19.331 −13.578 1.00 6.98 ATOM 1784 CG ASP A 372 8.931 −19.840 −14.888 1.00 6.85 ATOM 1785 OD1 ASP A 372 10.130 −20.026 −15.077 1.00 8.43 ATOM 1786 OD2 ASP A 372 7.994 −19.987 −15.770 1.00 8.15 ATOM 1787 C ASP A 372 10.552 −18.144 −12.844 1.00 7.36 ATOM 1788 O ASP A 372 10.260 −16.943 −13.113 1.00 6.89 ATOM 1789 N SER A 373 11.789 −18.611 −13.057 1.00 7.22 ATOM 1790 CA SER A 373 12.904 −17.824 −13.435 1.00 7.27 ATOM 1791 CB SER A 373 14.212 −18.514 −13.097 1.00 8.45 ATOM 1792 OG SER A 373 14.306 −19.703 −13.923 1.00 10.38 ATOM 1793 C SER A 373 12.855 −17.252 −14.831 1.00 7.13 ATOM 1794 O SER A 373 13.729 −16.486 −15.246 1.00 8.16 ATOM 1795 N GLU A 374 11.848 −17.684 −15.609 1.00 6.59 ATOM 1796 CA GLU A 374 11.550 −17.008 −16.876 1.00 6.73 ATOM 1797 CB GLU A 374 10.529 −17.795 −17.698 1.00 7.58 ATOM 1798 CG GLU A 374 11.052 −19.129 −18.224 1.00 8.39 ATOM 1799 CD GLU A 374 12.152 −18.969 −19.175 1.00 9.22 ATOM 1800 OE1 GLU A 374 11.868 −18.586 −20.360 1.00 12.84 ATOM 1801 OE2 GLU A 374 13.336 −19.052 −18.871 1.00 11.82 ATOM 1802 C GLU A 374 10.998 −15.588 −16.642 1.00 6.60 ATOM 1803 O GLU A 374 10.897 −14.774 −17.590 1.00 7.38 ATOM 1804 N GLY A 375 10.609 −15.248 −15.404 1.00 6.07 ATOM 1805 CA GLY A 375 10.131 −13.891 −15.077 1.00 6.30 ATOM 1806 C GLY A 375 11.252 −12.887 −14.941 1.00 5.58 ATOM 1807 O GLY A 375 12.423 −13.169 −15.175 1.00 6.01 ATOM 1808 N VAL A 376 10.843 −11.743 −14.426 1.00 5.53 ATOM 1809 CA VAL A 376 11.732 −10.617 −14.162 1.00 5.61 ATOM 1810 CB VAL A 376 11.093 −9.264 −14.596 1.00 5.00 ATOM 1811 CG1 VAL A 376 11.873 −8.097 −14.061 1.00 5.19 ATOM 1812 CG2 VAL A 376 10.933 −9.218 −16.085 1.00 6.21 ATOM 1813 C VAL A 376 12.110 −10.617 −12.689 1.00 5.01 ATOM 1814 O VAL A 376 11.229 −10.626 −11.824 1.00 4.97 ATOM 1815 N LEU A 377 13.416 −10.709 −12.425 1.00 5.13 ATOM 1816 CA LEU A 377 13.896 −10.809 −11.043 1.00 5.03 ATOM 1817 CB LEU A 377 15.204 −11.574 −11.030 1.00 5.59 ATOM 1818 CG LEU A 377 15.828 −11.880 −9.665 1.00 5.86 ATOM 1819 CD1 LEU A 377 14.988 −12.738 −8.806 1.00 6.64 ATOM 1820 CD2 LEU A 377 17.205 −12.513 −9.802 1.00 6.55 ATOM 1821 C LEU A 377 14.196 −9.416 −10.459 1.00 4.22 ATOM 1822 O LEU A 377 14.775 −8.569 −11.109 1.00 5.02 ATOM 1823 N SER A 378 13.674 −9.211 −9.248 1.00 4.66 ATOM 1824 CA SER A 378 13.896 −7.927 −8.561 1.00 4.58 ATOM 1825 CB SER A 378 12.932 −6.879 −9.083 1.00 4.79 ATOM 1826 OG SER A 378 13.362 −5.598 −8.714 1.00 5.38 ATOM 1827 C SER A 378 13.842 −8.136 −7.067 1.00 5.15 ATOM 1828 O SER A 378 13.609 −9.209 −6.539 1.00 5.53 ATOM 1829 N THR A 379 14.029 −7.002 −6.355 1.00 5.21 ATOM 1830 CA THR A 379 13.815 −6.914 −4.906 1.00 5.09 ATOM 1831 CB THR A 379 14.208 −5.506 −4.421 1.00 4.92 ATOM 1832 OG1 THR A 379 13.675 −4.509 −5.324 1.00 5.01 ATOM 1833 CG2 THR A 379 15.725 −5.307 −4.451 1.00 5.43 ATOM 1834 C THR A 379 12.445 −7.253 −4.526 1.00 5.05 ATOM 1835 O THR A 379 11.463 −6.850 −5.236 1.00 6.03 ATOM 1836 N TYR A 380 12.222 −7.897 −3.410 1.00 5.22 ATOM 1837 CA TYR A 380 10.961 −8.408 −3.024 1.00 5.45 ATOM 1838 CB TYR A 380 10.698 −9.773 −3.748 1.00 6.80 ATOM 1839 CG TYR A 380 9.345 −10.248 −3.896 1.00 9.74 ATOM 1840 CD1 TYR A 380 8.333 −9.427 −4.410 1.00 11.08 ATOM 1841 CE1 TYR A 380 6.963 −10.076 −4.475 1.00 14.44 ATOM 1842 CZ TYR A 380 6.890 −11.401 −3.950 1.00 12.17 ATOM 1843 OH TYR A 380 5.742 −12.325 −3.912 1.00 22.38 ATOM 1844 CE2 TYR A 380 7.840 −12.107 −3.542 1.00 16.42 ATOM 1845 CD2 TYR A 380 9.063 −11.556 −3.488 1.00 10.82 ATOM 1846 C TYR A 380 10.853 −8.596 −1.510 1.00 5.43 ATOM 1847 O TYR A 380 11.788 −8.158 −0.817 1.00 6.11 ATOM 1848 N ASN A 381 9.805 −9.145 −0.985 1.00 5.77 ATOM 1849 CA ASN A 381 9.661 −9.365 0.457 1.00 5.95 ATOM 1850 CB ASN A 381 8.777 −8.237 1.040 1.00 6.34 ATOM 1851 CG ASN A 381 8.832 −8.237 2.577 1.00 5.59 ATOM 1852 OD1 ASN A 381 8.121 −8.985 3.226 1.00 6.24 ATOM 1853 ND2 ASN A 381 9.684 −7.389 3.153 1.00 5.15 ATOM 1854 C ASN A 381 9.053 −10.699 0.645 1.00 6.60 ATOM 1855 O ASN A 381 8.199 −11.112 −0.175 1.00 6.82 ATOM 1856 N SER A 382 9.412 −11.393 1.692 1.00 6.99 ATOM 1857 CA SER A 382 8.909 −12.745 1.978 1.00 6.67 ATOM 1858 CB SER A 382 9.939 −13.547 2.755 1.00 7.93 ATOM 1859 OG SER A 382 10.112 −13.002 4.054 1.00 9.98 ATOM 1860 C SER A 382 7.622 −12.797 2.677 1.00 7.50 ATOM 1861 O SER A 382 7.041 −13.881 2.906 1.00 8.69 ATOM 1862 N GLY A 383 7.060 −11.660 3.151 1.00 7.27 ATOM 1863 CA GLY A 383 5.859 −11.700 3.970 1.00 7.34 ATOM 1864 C GLY A 383 4.639 −12.138 3.181 1.00 8.08 ATOM 1865 O GLY A 383 4.375 −11.591 2.088 1.00 8.91 ATOM 1866 N SER A 384 3.773 −12.969 3.716 1.00 8.84 ATOM 1867 CA SER A 384 2.523 −13.285 3.052 1.00 9.88 ATOM 1868 CB SER A 384 1.979 −14.643 3.565 1.00 12.08 ATOM 1869 OG SER A 384 1.901 −14.613 4.906 1.00 17.04 ATOM 1870 C SER A 384 1.535 −12.195 3.235 1.00 9.08 ATOM 1871 O SER A 384 0.666 −12.021 2.345 1.00 9.90 ATOM 1872 N SER A 385 1.520 −11.431 4.315 1.00 8.60 ATOM 1873 CA SER A 385 0.568 −10.367 4.564 1.00 9.88 ATOM 1874 CB SER A 385 −0.572 −10.739 5.532 1.00 12.16 ATOM 1875 OG SER A 385 −0.049 −11.315 6.724 1.00 15.59 ATOM 1876 C SER A 385 1.327 −9.195 5.039 1.00 9.40 ATOM 1877 O SER A 385 1.659 −8.242 4.297 1.00 9.02 ATOM 1878 N THR A 386 1.696 −9.162 6.336 1.00 9.23 ATOM 1879 CA THR A 386 2.604 −8.189 6.882 1.00 9.50 ATOM 1880 CB THR A 386 2.563 −8.228 8.435 1.00 8.72 ATOM 1881 OG1 THR A 386 2.709 −9.590 8.816 1.00 10.92 ATOM 1882 CG2 THR A 386 1.160 −7.755 8.878 1.00 9.99 ATOM 1883 C THR A 386 4.023 −8.337 6.327 1.00 8.52 ATOM 1884 O THR A 386 4.425 −9.453 5.959 1.00 8.06 ATOM 1885 N PRO A 387 4.800 −7.272 6.326 1.00 8.01 ATOM 1886 CA PRO A 387 6.164 −7.403 5.780 1.00 7.77 ATOM 1887 CB PRO A 387 6.695 −5.995 5.727 1.00 9.07 ATOM 1888 CG PRO A 387 5.798 −5.151 6.539 1.00 12.35 ATOM 1889 CD PRO A 387 4.521 −5.906 6.751 1.00 8.95 ATOM 1890 C PRO A 387 7.037 −8.309 6.640 1.00 8.59 ATOM 1891 O PRO A 387 6.882 −8.323 7.861 1.00 9.69 ATOM 1892 N SER A 388 7.951 −9.017 6.039 1.00 7.22 ATOM 1893 CA SER A 388 8.928 −9.882 6.719 1.00 6.84 ATOM 1894 CB SER A 388 8.431 −11.309 6.640 1.00 8.27 ATOM 1895 OG SER A 388 9.252 −12.193 7.377 1.00 9.88 ATOM 1896 C SER A 388 10.278 −9.624 6.082 1.00 7.61 ATOM 1897 O SER A 388 10.605 −8.534 5.608 1.00 6.35 ATOM 1898 N SER A 389 11.104 −10.654 6.108 1.00 8.44 ATOM 1899 CA SER A 389 12.462 −10.520 5.592 1.00 8.39 ATOM 1900 CB SER A 389 13.261 −11.784 5.879 1.00 12.55 ATOM 1901 OG SER A 389 12.695 −12.796 5.208 1.00 18.53 ATOM 1902 C SER A 389 12.538 −10.123 4.124 1.00 7.34 ATOM 1903 O SER A 389 11.664 −10.516 3.295 1.00 7.95 ATOM 1904 N ASP A 390 13.584 −9.443 3.743 1.00 6.91 ATOM 1905 CA ASP A 390 13.935 −9.125 2.347 1.00 7.02 ATOM 1906 CB ASP A 390 15.207 −8.345 2.258 1.00 7.16 ATOM 1907 CG ASP A 390 15.146 −6.926 2.845 1.00 7.70 ATOM 1908 OD1 ASP A 390 14.032 −6.368 2.946 1.00 8.25 ATOM 1909 OD2 ASP A 390 16.253 −6.362 3.057 1.00 9.23 ATOM 1910 C ASP A 390 14.061 −10.437 1.579 1.00 7.82 ATOM 1911 O ASP A 390 14.646 −11.416 2.055 1.00 9.20 ATOM 1912 N SER A 391 13.676 −10.398 0.305 1.00 7.19 ATOM 1913 CA SER A 391 13.917 −11.510 −0.621 1.00 6.84 ATOM 1914 CB SER A 391 12.758 −12.470 −0.523 1.00 7.48 ATOM 1915 OG SER A 391 11.582 −11.872 −1.031 1.00 8.47 ATOM 1916 C SER A 391 14.143 −10.971 −2.006 1.00 6.79 ATOM 1917 O SER A 391 14.109 −9.745 −2.262 1.00 6.77 ATOM 1918 N TYR A 392 14.363 −11.870 −2.952 1.00 6.93 ATOM 1919 CA TYR A 392 14.386 −11.585 −4.392 1.00 6.68 ATOM 1920 CB TYR A 392 15.773 −11.762 −4.990 1.00 7.56 ATOM 1921 CG TYR A 392 16.812 −10.948 −4.295 1.00 7.71 ATOM 1922 CD1 TYR A 392 17.016 −9.588 −4.546 1.00 6.96 ATOM 1923 CE1 TYR A 392 17.904 −8.826 −3.813 1.00 6.32 ATOM 1924 CZ TYR A 392 18.662 −9.397 −2.886 1.00 6.71 ATOM 1925 OH TYR A 392 19.502 −8.564 −2.187 1.00 9.05 ATOM 1926 CE2 TYR A 392 18.469 −10.695 −2.544 1.00 8.98 ATOM 1927 CD2 TYR A 392 17.625 −11.511 −3.279 1.00 8.30 ATOM 1928 C TYR A 392 13.413 −12.445 −5.053 1.00 7.98 ATOM 1929 O TYR A 392 13.297 −13.664 −4.688 1.00 10.80 ATOM 1930 N GLY A 393 12.612 −11.982 −5.981 1.00 6.52 ATOM 1931 CA GLY A 393 11.597 −12.776 −6.604 1.00 6.63 ATOM 1932 C GLY A 393 11.382 −12.440 −8.044 1.00 6.07 ATOM 1933 O GLY A 393 11.683 −11.353 −8.520 1.00 5.85 ATOM 1934 N TYR A 394 10.936 −13.479 −8.784 1.00 5.56 ATOM 1935 CA TYR A 394 10.596 −13.362 −10.185 1.00 5.99 ATOM 1936 CB TYR A 394 10.964 −14.643 −11.000 1.00 6.91 ATOM 1937 CG TYR A 394 12.382 −15.044 −11.012 1.00 6.47 ATOM 1938 CD1 TYR A 394 13.297 −14.561 −11.946 1.00 6.66 ATOM 1939 CE1 TYR A 394 14.625 −14.990 −11.960 1.00 8.30 ATOM 1940 CZ TYR A 394 14.999 −15.927 −11.043 1.00 8.15 ATOM 1941 OH TYR A 394 16.370 −16.318 −11.104 1.00 11.44 ATOM 1942 CE2 TYR A 394 14.182 −16.415 −10.130 1.00 8.74 ATOM 1943 CD2 TYR A 394 12.852 −15.997 −10.095 1.00 8.35 ATOM 1944 C TYR A 394 9.129 −13.120 −10.366 1.00 5.83 ATOM 1945 O TYR A 394 8.312 −13.864 −9.873 1.00 7.06 ATOM 1946 N SER A 395 8.789 −12.030 −11.083 1.00 6.38 ATOM 1947 CA SER A 395 7.410 −11.655 −11.324 1.00 6.59 ATOM 1948 CB SER A 395 7.045 −10.405 −10.576 1.00 8.97 ATOM 1949 OG SER A 395 6.919 −10.642 −9.151 1.00 13.33 ATOM 1950 C SER A 395 7.228 −11.449 −12.813 1.00 6.43 ATOM 1951 O SER A 395 8.189 −11.402 −13.568 1.00 8.06 ATOM 1952 N GLN A 396 6.018 −11.234 −13.251 1.00 6.41 ATOM 1953 CA GLN A 396 5.745 −10.944 −14.668 1.00 6.44 ATOM 1954 CB GLN A 396 5.357 −12.172 −15.456 1.00 6.50 ATOM 1955 CG GLN A 396 4.057 −12.792 −15.065 1.00 7.36 ATOM 1956 CD GLN A 396 3.571 −13.898 −15.978 1.00 7.88 ATOM 1957 OE1 GLN A 396 4.180 −14.266 −16.934 1.00 7.28 ATOM 1958 NE2 GLN A 396 2.428 −14.389 −15.657 1.00 11.12 ATOM 1959 C GLN A 396 4.643 −9.914 −14.835 1.00 5.38 ATOM 1960 O GLN A 396 3.783 −9.791 −13.980 1.00 6.64 ATOM 1961 N GLY A 397 4.689 −9.226 −15.992 1.00 4.84 ATOM 1962 CA GLY A 397 3.613 −8.328 −16.342 1.00 5.25 ATOM 1963 C GLY A 397 4.143 −7.219 −17.137 1.00 5.02 ATOM 1964 O GLY A 397 5.333 −6.880 −17.217 1.00 5.60 ATOM 1965 N THR A 398 3.204 −6.472 −17.754 1.00 5.55 ATOM 1966 CA THR A 398 3.530 −5.130 −18.270 1.00 4.79 ATOM 1967 CB THR A 398 2.427 −4.471 −19.086 1.00 4.92 ATOM 1968 OG1 THR A 398 1.285 −4.339 −18.283 1.00 5.20 ATOM 1969 CG2 THR A 398 2.144 −5.195 −20.366 1.00 5.57 ATOM 1970 C THR A 398 4.033 −4.237 −17.118 1.00 4.88 ATOM 1971 O THR A 398 4.825 −3.323 −17.413 1.00 5.00 ATOM 1972 N SER A 399 3.703 −4.552 −15.887 1.00 5.22 ATOM 1973 CA SER A 399 4.226 −3.835 −14.733 1.00 5.52 ATOM 1974 CB SER A 399 3.657 −4.424 −13.435 1.00 8.77 ATOM 1975 OG SER A 399 2.353 −3.974 −13.180 1.00 12.78 ATOM 1976 C SER A 399 5.738 −3.966 −14.566 1.00 4.94 ATOM 1977 O SER A 399 6.315 −3.147 −13.933 1.00 5.21 ATOM 1978 N MET A 400 6.311 −5.061 −15.084 1.00 4.60 ATOM 1979 CA MET A 400 7.734 −5.351 −15.026 1.00 4.72 ATOM 1980 CB MET A 400 8.024 −6.828 −14.910 1.00 5.52 ATOM 1981 CG MET A 400 7.841 −7.498 −13.526 1.00 6.38 ATOM 1982 SD MET A 400 6.169 −7.493 −12.849 1.00 7.01 ATOM 1983 CE MET A 400 6.307 −6.177 −11.679 1.00 6.72 ATOM 1984 C MET A 400 8.427 −4.765 −16.224 1.00 4.53 ATOM 1985 O MET A 400 9.601 −4.377 −16.161 1.00 5.03 ATOM 1986 N ALA A 401 7.722 −4.678 −17.355 1.00 4.25 ATOM 1987 CA ALA A 401 8.281 −4.076 −18.574 1.00 4.33 ATOM 1988 CB ALA A 401 7.357 −4.428 −19.751 1.00 4.78 ATOM 1989 C ALA A 401 8.417 −2.561 −18.415 1.00 4.22 ATOM 1990 O ALA A 401 9.434 −1.965 −18.772 1.00 4.88 ATOM 1991 N ALA A 402 7.392 −1.891 −17.896 1.00 4.43 ATOM 1992 CA ALA A 402 7.407 −0.435 −17.727 1.00 4.11 ATOM 1993 CB ALA A 402 6.098 0.050 −17.092 1.00 4.65 ATOM 1994 C ALA A 402 8.644 0.100 −16.992 1.00 3.62 ATOM 1995 O ALA A 402 9.258 1.027 −17.517 1.00 4.15 ATOM 1996 N PRO A 403 9.047 −0.477 −15.883 1.00 4.16 ATOM 1997 CA PRO A 403 10.235 0.126 −15.138 1.00 4.22 ATOM 1998 CB PRO A 403 10.201 −0.554 −13.781 1.00 4.57 ATOM 1999 CG PRO A 403 9.395 −1.803 −14.036 1.00 4.53 ATOM 2000 CD PRO A 403 8.326 −1.392 −14.952 1.00 4.15 ATOM 2001 C PRO A 403 11.500 −0.083 −15.910 1.00 4.28 ATOM 2002 O PRO A 403 12.444 0.690 −15.681 1.00 4.72 ATOM 2003 N HIS A 404 11.607 −1.067 −16.779 1.00 3.86 ATOM 2004 CA HIS A 404 12.775 −1.103 −17.704 1.00 4.22 ATOM 2005 CB HIS A 404 12.739 −2.357 −18.578 1.00 4.58 ATOM 2006 CG HIS A 404 13.099 −3.652 −17.891 1.00 4.50 ATOM 2007 ND1 HIS A 404 12.222 −4.465 −17.259 1.00 4.78 ATOM 2008 CE1 HIS A 404 12.867 −5.528 −16.798 1.00 5.00 ATOM 2009 NE2 HIS A 404 14.131 −5.445 −17.107 1.00 5.17 ATOM 2010 CD2 HIS A 404 14.316 −4.281 −17.773 1.00 4.60 ATOM 2011 C HIS A 404 12.796 0.139 −18.513 1.00 4.49 ATOM 2012 O HIS A 404 13.876 0.765 −18.734 1.00 5.08 ATOM 2013 N VAL A 405 11.659 0.521 −19.089 1.00 4.72 ATOM 2014 CA VAL A 405 11.579 1.708 −19.921 1.00 4.32 ATOM 2015 CB VAL A 405 10.271 1.705 −20.712 1.00 4.22 ATOM 2016 CG1 VAL A 405 10.120 2.964 −21.577 1.00 4.10 ATOM 2017 CG2 VAL A 405 10.154 0.450 −21.499 1.00 4.70 ATOM 2018 C VAL A 405 11.765 3.000 −19.093 1.00 4.25 ATOM 2019 O VAL A 405 12.469 3.905 −19.577 1.00 4.89 ATOM 2020 N ALA A 406 11.219 3.049 −17.906 1.00 4.25 ATOM 2021 CA ALA A 406 11.428 4.215 −17.043 1.00 4.05 ATOM 2022 CB ALA A 406 10.571 4.093 −15.795 1.00 4.77 ATOM 2023 C ALA A 406 12.888 4.343 −16.710 1.00 4.63 ATOM 2024 O ALA A 406 13.404 5.445 −16.684 1.00 5.04 ATOM 2025 N GLY A 407 13.555 3.191 −16.487 1.00 4.58 ATOM 2026 CA GLY A 407 14.998 3.295 −16.209 1.00 5.33 ATOM 2027 C GLY A 407 15.733 3.806 −17.428 1.00 5.26 ATOM 2028 O GLY A 407 16.654 4.633 −17.281 1.00 5.34 ATOM 2029 N VAL A 408 15.423 3.349 −18.650 1.00 4.99 ATOM 2030 CA VAL A 408 16.076 3.932 −19.826 1.00 5.08 ATOM 2031 CB VAL A 408 15.821 3.073 −21.089 1.00 5.40 ATOM 2032 CG1 VAL A 408 16.503 3.694 −22.296 1.00 5.87 ATOM 2033 CG2 VAL A 408 16.324 1.649 −20.882 1.00 5.54 ATOM 2034 C VAL A 408 15.758 5.382 −19.983 1.00 5.05 ATOM 2035 O VAL A 408 16.653 6.159 −20.399 1.00 4.96 ATOM 2036 N ALA A 409 14.529 5.817 −19.689 1.00 5.38 ATOM 2037 CA ALA A 409 14.254 7.249 −19.688 1.00 5.24 ATOM 2038 CB ALA A 409 12.782 7.481 −19.311 1.00 5.22 ATOM 2039 C ALA A 409 15.182 8.015 −18.770 1.00 5.02 ATOM 2040 O ALA A 409 15.628 9.128 −19.130 1.00 6.44 ATOM 2041 N ALA A 410 15.502 7.473 −17.610 1.00 5.06 ATOM 2042 CA ALA A 410 16.469 8.067 −16.725 1.00 5.41 ATOM 2043 CB ALA A 410 16.425 7.401 −15.333 1.00 5.91 ATOM 2044 C ALA A 410 17.845 8.056 −17.316 1.00 5.75 ATOM 2045 O ALA A 410 18.588 9.072 −17.096 1.00 6.84 ATOM 2046 N LEU A 411 18.291 7.025 −17.993 1.00 5.45 ATOM 2047 CA LEU A 411 19.605 7.010 −18.653 1.00 6.46 ATOM 2048 CB LEU A 411 19.901 5.686 −19.299 1.00 6.18 ATOM 2049 CG LEU A 411 19.846 4.467 −18.417 1.00 6.63 ATOM 2050 CD1 LEU A 411 20.325 3.235 −19.173 1.00 7.08 ATOM 2051 CD2 LEU A 411 20.658 4.646 −17.136 1.00 7.52 ATOM 2052 C LEU A 411 19.629 8.144 −19.651 1.00 6.50 ATOM 2053 O LEU A 411 20.645 8.898 −19.762 1.00 7.27 ATOM 2054 N ILE A 412 18.623 8.278 −20.476 1.00 5.77 ATOM 2055 CA ILE A 412 18.505 9.355 −21.444 1.00 5.97 ATOM 2056 CB ILE A 412 17.145 9.203 −22.257 1.00 6.18 ATOM 2057 CG1 ILE A 412 17.189 8.011 −23.142 1.00 6.34 ATOM 2058 CD1 ILE A 412 15.780 7.579 −23.614 1.00 6.50 ATOM 2059 CG2 ILE A 412 16.839 10.490 −22.988 1.00 6.87 ATOM 2060 C ILE A 412 18.600 10.653 −20.774 1.00 6.44 ATOM 2061 O ILE A 412 19.309 11.595 −21.315 1.00 7.45 ATOM 2062 N LYS A 413 17.914 10.913 −19.683 1.00 6.35 ATOM 2063 CA LYS A 413 17.923 12.205 −19.007 1.00 7.03 ATOM 2064 CB LYS A 413 16.830 12.243 −17.930 1.00 7.67 ATOM 2065 CG LYS A 413 16.578 13.575 −17.344 1.00 8.21 ATOM 2066 CD LYS A 413 15.950 14.540 −18.325 1.00 9.83 ATOM 2067 CE LYS A 413 15.470 15.807 −17.618 1.00 13.68 ATOM 2068 NZ LYS A 413 14.946 16.795 −18.486 1.00 21.99 ATOM 2069 C LYS A 413 19.312 12.504 −18.438 1.00 6.77 ATOM 2070 O LYS A 413 19.664 13.698 −18.418 1.00 8.52 ATOM 2071 N GLN A 414 20.069 11.556 −17.995 1.00 6.56 ATOM 2072 CA GLN A 414 21.472 11.845 −17.577 1.00 7.27 ATOM 2073 CB GLN A 414 22.121 10.645 −16.994 1.00 7.49 ATOM 2074 CG GLN A 414 23.585 10.911 −16.575 1.00 7.79 ATOM 2075 CD GLN A 414 24.341 9.708 −16.248 1.00 9.61 ATOM 2076 OE1 GLN A 414 23.861 8.619 −16.029 1.00 9.10 ATOM 2077 NE2 GLN A 414 25.622 9.855 −16.074 1.00 12.19 ATOM 2078 C GLN A 414 22.261 12.325 −18.776 1.00 8.57 ATOM 2079 O GLN A 414 22.975 13.329 −18.696 1.00 10.04 ATOM 2080 N ALA A 415 22.137 11.653 −19.900 1.00 7.66 ATOM 2081 CA ALA A 415 22.903 12.014 −21.121 1.00 8.36 ATOM 2082 CB ALA A 415 22.806 10.894 −22.121 1.00 9.35 ATOM 2083 C ALA A 415 22.434 13.308 −21.689 1.00 8.81 ATOM 2084 O ALA A 415 23.258 14.053 −22.304 1.00 11.11 ATOM 2085 N LYS A 416 21.185 13.684 −21.603 1.00 9.15 ATOM 2086 CA LYS A 416 20.550 14.869 −22.199 1.00 9.41 ATOM 2087 CB LYS A 416 19.728 14.425 −23.408 1.00 10.67 ATOM 2088 CG LYS A 416 19.200 15.600 −24.224 1.00 11.77 ATOM 2089 CD LYS A 416 18.465 15.092 −25.470 1.00 15.03 ATOM 2090 CE LYS A 416 17.665 16.131 −26.256 1.00 17.11 ATOM 2091 NZ LYS A 416 18.609 17.177 −26.705 1.00 20.59 ATOM 2092 C LYS A 416 19.708 15.537 −21.113 1.00 10.25 ATOM 2093 O LYS A 416 18.476 15.414 −21.085 1.00 8.78 ATOM 2094 N PRO A 417 20.327 16.334 −20.195 1.00 9.48 ATOM 2095 CA PRO A 417 19.619 16.834 −19.068 1.00 9.79 ATOM 2096 CB PRO A 417 20.718 17.453 −18.181 1.00 11.93 ATOM 2097 CG PRO A 417 21.988 16.966 −18.681 1.00 16.04 ATOM 2098 CD PRO A 417 21.795 16.467 −20.086 1.00 13.11 ATOM 2099 C PRO A 417 18.531 17.814 −19.382 1.00 10.64 ATOM 2100 O PRO A 417 17.681 18.075 −18.526 1.00 13.90 ATOM 2101 N ASP A 418 18.511 18.419 −20.550 1.00 11.72 ATOM 2102 CA ASP A 418 17.411 19.290 −20.936 1.00 12.79 ATOM 2103 CB ASP A 418 17.999 20.511 −21.728 1.00 18.32 ATOM 2104 CG ASP A 418 18.480 20.151 −23.150 1.00 23.99 ATOM 2105 OD1 ASP A 418 18.618 18.963 −23.660 1.00 28.19 ATOM 2106 OD2 ASP A 418 18.853 21.136 −23.899 1.00 31.60 ATOM 2107 C ASP A 418 16.365 18.617 −21.804 1.00 11.74 ATOM 2108 O ASP A 418 15.468 19.249 −22.312 1.00 11.92 ATOM 2109 N ALA A 419 16.434 17.281 −21.856 1.00 10.77 ATOM 2110 CA ALA A 419 15.403 16.585 −22.704 1.00 11.21 ATOM 2111 CB ALA A 419 15.706 15.083 −22.738 1.00 11.26 ATOM 2112 C ALA A 419 13.965 16.770 −22.238 1.00 9.90 ATOM 2113 O ALA A 419 13.734 16.670 −21.011 1.00 11.77 ATOM 2114 N THR A 420 13.052 17.084 −23.160 1.00 8.21 ATOM 2115 CA THR A 420 11.647 17.227 −22.827 1.00 7.27 ATOM 2116 CB THR A 420 10.968 18.114 −23.864 1.00 7.99 ATOM 2117 OG1 THR A 420 10.956 17.440 −25.094 1.00 9.57 ATOM 2118 CG2 THR A 420 11.638 19.525 −23.990 1.00 9.92 ATOM 2119 C THR A 420 10.952 15.869 −22.808 1.00 7.75 ATOM 2120 O THR A 420 11.463 14.907 −23.354 1.00 7.20 ATOM 2121 N PRO A 421 9.741 15.809 −22.267 1.00 6.91 ATOM 2122 CA PRO A 421 9.030 14.542 −22.321 1.00 7.18 ATOM 2123 CB PRO A 421 7.686 14.884 −21.625 1.00 7.84 ATOM 2124 CG PRO A 421 8.106 15.914 −20.598 1.00 9.17 ATOM 2125 CD PRO A 421 9.112 16.789 −21.319 1.00 7.70 ATOM 2126 C PRO A 421 8.843 14.036 −23.719 1.00 6.94 ATOM 2127 O PRO A 421 8.959 12.829 −23.961 1.00 6.85 ATOM 2128 N ASP A 422 8.519 14.909 −24.692 1.00 6.44 ATOM 2129 CA ASP A 422 8.360 14.452 −26.035 1.00 7.05 ATOM 2130 CB ASP A 422 7.767 15.598 −26.911 1.00 8.15 ATOM 2131 CG ASP A 422 6.339 15.787 −26.642 1.00 11.35 ATOM 2132 OD1 ASP A 422 5.606 14.957 −26.063 1.00 10.75 ATOM 2133 OD2 ASP A 422 5.815 16.849 −27.063 1.00 15.59 ATOM 2134 C ASP A 422 9.649 14.017 −26.645 1.00 6.66 ATOM 2135 O ASP A 422 9.688 12.998 −27.371 1.00 7.08 ATOM 2136 N GLU A 423 10.767 14.621 −26.312 1.00 6.12 ATOM 2137 CA GLU A 423 12.042 14.203 −26.850 1.00 6.67 ATOM 2138 CB GLU A 423 13.168 15.180 −26.533 1.00 8.75 ATOM 2139 CG GLU A 423 13.123 16.446 −27.364 1.00 10.35 ATOM 2140 CD GLU A 423 14.034 17.503 −26.862 1.00 12.60 ATOM 2141 OE1 GLU A 423 14.456 17.654 −25.734 1.00 12.26 ATOM 2142 OE2 GLU A 423 14.325 18.354 −27.781 1.00 18.79 ATOM 2143 C GLU A 423 12.432 12.852 −26.256 1.00 6.54 ATOM 2144 O GLU A 423 12.970 11.971 −26.968 1.00 7.50 ATOM 2145 N ILE A 424 12.180 12.575 −24.974 1.00 6.43 ATOM 2146 CA ILE A 424 12.473 11.288 −24.379 1.00 6.44 ATOM 2147 CB ILE A 424 12.390 11.314 −22.859 1.00 6.56 ATOM 2148 CG1 ILE A 424 13.317 12.351 −22.241 1.00 7.50 ATOM 2149 CD1 ILE A 424 13.138 12.541 −20.740 1.00 9.21 ATOM 2150 CG2 ILE A 424 12.545 9.904 −22.315 1.00 7.02 ATOM 2151 C ILE A 424 11.655 10.202 −25.009 1.00 6.24 ATOM 2152 O ILE A 424 12.201 9.142 −25.379 1.00 5.94 ATOM 2153 N GLU A 425 10.362 10.429 −25.170 1.00 5.37 ATOM 2154 CA GLU A 425 9.527 9.441 −25.849 1.00 6.14 ATOM 2155 CB GLU A 425 8.068 9.900 −25.874 1.00 6.26 ATOM 2156 CG GLU A 425 7.149 8.936 −26.591 1.00 6.56 ATOM 2157 CD GLU A 425 5.714 9.151 −26.375 1.00 6.68 ATOM 2158 OE1 GLU A 425 5.291 10.164 −25.795 1.00 8.38 ATOM 2159 OE2 GLU A 425 4.907 8.280 −26.808 1.00 7.91 ATOM 2160 C GLU A 425 10.065 9.167 −27.260 1.00 6.41 ATOM 2161 O GLU A 425 10.118 8.024 −27.727 1.00 5.98 ATOM 2162 N SER A 426 10.383 10.248 −27.983 1.00 6.62 ATOM 2163 CA SER A 426 10.915 10.066 −29.365 1.00 6.85 ATOM 2164 CB SER A 426 11.069 11.399 −29.997 1.00 7.67 ATOM 2165 OG SER A 426 11.535 11.139 −31.363 1.00 10.25 ATOM 2166 C SER A 426 12.177 9.266 −29.361 1.00 6.57 ATOM 2167 O SER A 426 12.355 8.358 −30.218 1.00 6.87 ATOM 2168 N ILE A 427 13.113 9.503 −28.495 1.00 6.43 ATOM 2169 CA ILE A 427 14.352 8.740 −28.435 1.00 6.80 ATOM 2170 CB ILE A 427 15.370 9.352 −27.400 1.00 6.80 ATOM 2171 CG1 ILE A 427 15.890 10.674 −27.910 1.00 7.21 ATOM 2172 CD1 ILE A 427 16.525 11.558 −26.848 1.00 8.19 ATOM 2173 CG2 ILE A 427 16.502 8.384 −27.141 1.00 6.81 ATOM 2174 C ILE A 427 14.074 7.303 −28.137 1.00 6.44 ATOM 2175 O ILE A 427 14.590 6.377 −28.811 1.00 6.82 ATOM 2176 N LEU A 428 13.205 6.997 −27.165 1.00 6.08 ATOM 2177 CA LEU A 428 12.851 5.612 −26.813 1.00 5.80 ATOM 2178 CB LEU A 428 11.852 5.594 −25.704 1.00 6.09 ATOM 2179 CG LEU A 428 12.406 5.931 −24.271 1.00 6.74 ATOM 2180 CD1 LEU A 428 11.233 6.197 −23.330 1.00 6.27 ATOM 2181 CD2 LEU A 428 13.294 4.805 −23.704 1.00 7.01 ATOM 2182 C LEU A 428 12.274 4.921 −28.014 1.00 5.65 ATOM 2183 O LEU A 428 12.633 3.779 −28.331 1.00 6.90 ATOM 2184 N LYS A 429 11.315 5.543 −28.718 1.00 5.38 ATOM 2185 CA LYS A 429 10.595 4.941 −29.842 1.00 6.49 ATOM 2186 CB LYS A 429 9.391 5.770 −30.194 1.00 7.23 ATOM 2187 CG LYS A 429 8.306 5.715 −29.112 1.00 7.72 ATOM 2188 CD LYS A 429 7.010 6.502 −29.494 1.00 8.80 ATOM 2189 CE LYS A 429 6.322 5.785 −30.600 1.00 8.82 ATOM 2190 NZ LYS A 429 4.922 6.308 −30.697 1.00 10.82 ATOM 2191 C LYS A 429 11.509 4.800 −31.056 1.00 6.51 ATOM 2192 O LYS A 429 11.388 3.841 −31.798 1.00 7.02 ATOM 2193 N SER A 430 12.405 5.743 −31.262 1.00 6.54 ATOM 2194 CA SER A 430 13.165 5.771 −32.539 1.00 6.25 ATOM 2195 CB SER A 430 13.554 7.208 −32.868 1.00 7.79 ATOM 2196 OG SER A 430 12.500 8.159 −32.903 1.00 10.01 ATOM 2197 C SER A 430 14.362 4.888 −32.518 1.00 6.56 ATOM 2198 O SER A 430 14.966 4.687 −33.578 0.50 2.40 ATOM 2199 N THR A 431 14.790 4.375 −31.358 1.00 6.09 ATOM 2200 CA THR A 431 16.001 3.590 −31.217 1.00 6.75 ATOM 2201 CB THR A 431 16.974 4.240 −30.206 1.00 6.96 ATOM 2202 OG1 THR A 431 16.361 4.240 −28.911 1.00 6.77 ATOM 2203 CG2 THR A 431 17.327 5.634 −30.620 1.00 7.20 ATOM 2204 C THR A 431 15.769 2.140 −30.840 1.00 6.18 ATOM 2205 O THR A 431 16.692 1.436 −30.469 1.00 7.07 ATOM 2206 N THR A 432 14.520 1.689 −30.898 1.00 5.57 ATOM 2207 CA THR A 432 14.233 0.316 −30.537 1.00 5.90 ATOM 2208 CB THR A 432 12.731 0.022 −30.505 1.00 5.68 ATOM 2209 OG1 THR A 432 12.161 0.374 −31.778 1.00 6.07 ATOM 2210 CG2 THR A 432 12.006 0.742 −29.449 1.00 6.11 ATOM 2211 C THR A 432 14.882 −0.662 −31.572 1.00 5.96 ATOM 2212 O THR A 432 15.170 −0.280 −32.729 1.00 6.22 ATOM 2213 N ARG A 433 15.022 −1.863 −31.135 1.00 5.81 ATOM 2214 CA ARG A 433 15.557 −2.947 −31.962 1.00 6.25 ATOM 2215 CB ARG A 433 16.683 −3.717 −31.232 1.00 8.52 ATOM 2216 CG ARG A 433 16.184 −4.652 −30.087 1.00 10.34 ATOM 2217 CD ARG A 433 17.231 −5.356 −29.343 1.00 12.25 ATOM 2218 NE ARG A 433 16.576 −6.050 −28.273 1.00 11.04 ATOM 2219 CZ ARG A 433 16.180 −7.264 −28.174 1.00 9.82 ATOM 2220 NH1 ARG A 433 15.917 −8.082 −29.223 1.00 10.18 ATOM 2221 NH2 ARG A 433 15.928 −7.758 −26.988 1.00 8.10 ATOM 2222 C ARG A 433 14.446 −3.905 −32.335 1.00 6.54 ATOM 2223 O ARG A 433 13.319 −3.866 −31.847 1.00 7.19 ATOM 2224 N SER A 434 14.722 −4.767 −33.331 1.00 6.96 ATOM 2225 CA SER A 434 13.769 −5.689 −33.881 1.00 7.43 ATOM 2226 CB SER A 434 14.353 −6.432 −35.120 1.00 8.35 ATOM 2227 OG SER A 434 14.627 −5.522 −36.200 1.00 10.99 ATOM 2228 C SER A 434 13.287 −6.714 −32.863 1.00 6.79 ATOM 2229 O SER A 434 14.032 −7.145 −31.998 1.00 7.37 ATOM 2230 N PHE A 435 12.105 −7.197 −33.139 1.00 6.80 ATOM 2231 CA PHE A 435 11.556 −8.327 −32.412 1.00 6.87 ATOM 2232 CB PHE A 435 10.035 −8.346 −32.464 1.00 7.80 ATOM 2233 CG PHE A 435 9.355 −7.199 −31.764 1.00 7.41 ATOM 2234 CD1 PHE A 435 9.276 −7.148 −30.391 1.00 7.38 ATOM 2235 CE1 PHE A 435 8.627 −6.062 −29.814 1.00 7.96 ATOM 2236 CZ PHE A 435 8.102 −5.054 −30.600 1.00 7.41 ATOM 2237 CE2 PHE A 435 8.187 −5.105 −31.921 1.00 8.15 ATOM 2238 CD2 PHE A 435 8.791 −6.197 −32.514 1.00 7.70 ATOM 2239 C PHE A 435 12.101 −9.658 −33.006 1.00 7.63 ATOM 2240 O PHE A 435 11.855 −9.884 −34.178 1.00 10.06 ATOM 2241 N PRO A 436 12.698 −10.497 −32.221 1.00 8.01 ATOM 2242 CA PRO A 436 13.164 −11.805 −32.819 1.00 9.27 ATOM 2243 CB PRO A 436 14.280 −12.217 −31.889 1.00 11.50 ATOM 2244 CG PRO A 436 14.114 −11.504 −30.665 1.00 11.68 ATOM 2245 CD PRO A 436 13.365 −10.206 −30.912 1.00 8.73 ATOM 2246 C PRO A 436 12.061 −12.784 −32.868 1.00 9.59 ATOM 2247 O PRO A 436 12.333 −13.865 −33.471 1.00 12.13 ATOM 2248 N ALA A 437 10.911 −12.569 −32.274 1.00 8.84 ATOM 2249 CA ALA A 437 9.777 −13.415 −32.355 1.00 9.19 ATOM 2250 CB ALA A 437 9.707 −14.358 −31.155 1.00 8.71 ATOM 2251 C ALA A 437 8.547 −12.537 −32.486 1.00 11.27 ATOM 2252 O ALA A 437 8.594 −11.340 −32.091 1.00 10.81 ATOM 2253 N THR A 438 7.440 −13.112 −32.869 1.00 12.16 ATOM 2254 CA THR A 438 6.221 −12.365 −33.195 1.00 14.12 ATOM 2255 CB THR A 438 5.008 −13.386 −33.292 1.00 16.04 ATOM 2256 OG1 THR A 438 5.321 −14.495 −34.217 1.00 20.59 ATOM 2257 CG2 THR A 438 3.713 −12.721 −33.775 1.00 18.23 ATOM 2258 C THR A 438 5.847 −11.389 −32.004 1.00 11.16 ATOM 2259 O THR A 438 5.695 −11.861 −30.879 1.00 10.19 ATOM 2260 N CYS A 439 5.610 −10.130 −32.395 1.00 12.10 ATOM 2261 CA CYS A 439 5.018 −9.171 −31.441 1.00 10.50 ATOM 2262 CB CYS A 439 6.092 −8.464 −30.609 1.00 11.24 ATOM 2263 SG CYS A 439 5.618 −7.915 −28.974 1.00 10.33 ATOM 2264 C CYS A 439 4.138 −8.230 −32.242 1.00 12.27 ATOM 2265 O CYS A 439 4.649 −7.192 −32.684 1.00 13.32 ATOM 2266 N THR A 440 2.849 −8.625 −32.303 1.00 14.51 ATOM 2267 CA THR A 440 1.972 −7.830 −33.137 1.00 15.73 ATOM 2268 CB THR A 440 0.929 −8.613 −33.994 1.00 22.57 ATOM 2269 OG1 THR A 440 −0.429 −8.121 −33.938 1.00 32.58 ATOM 2270 CG2 THR A 440 0.986 −10.109 −33.846 1.00 20.48 ATOM 2271 C THR A 440 1.476 −6.618 −32.343 1.00 11.35 ATOM 2272 O THR A 440 0.995 −6.723 −31.238 1.00 12.33 ATOM 2273 N SER A 441 1.594 −5.535 −33.013 1.00 10.79 ATOM 2274 CA SER A 441 1.163 −4.245 −32.434 1.00 9.15 ATOM 2275 CB SER A 441 −0.302 −4.159 −32.369 1.00 9.92 ATOM 2276 OG SER A 441 −0.904 −4.274 −33.703 1.00 15.04 ATOM 2277 C SER A 441 1.761 −3.932 −31.061 1.00 9.15 ATOM 2278 O SER A 441 1.073 −3.632 −30.097 1.00 8.35 ATOM 2279 N CYS A 442 3.068 −4.115 −31.027 1.00 8.21 ATOM 2280 CA CYS A 442 3.895 −4.002 −29.833 1.00 8.21 ATOM 2281 CB CYS A 442 4.804 −5.172 −29.670 1.00 7.84 ATOM 2282 SG CYS A 442 3.967 −6.715 −29.278 1.00 10.08 ATOM 2283 C CYS A 442 4.752 −2.743 −29.882 1.00 7.62 ATOM 2284 O CYS A 442 5.840 −2.670 −29.229 1.00 7.69 ATOM 2285 N GLY A 443 4.346 −1.706 −30.591 1.00 7.48 ATOM 2286 CA GLY A 443 5.114 −0.498 −30.718 1.00 7.30 ATOM 2287 C GLY A 443 6.237 −0.701 −31.698 1.00 7.40 ATOM 2288 O GLY A 443 6.243 −1.613 −32.521 1.00 8.22 ATOM 2289 N THR A 444 7.254 0.155 −31.645 1.00 6.09 ATOM 2290 CA THR A 444 8.235 0.209 −32.733 1.00 6.11 ATOM 2291 CB THR A 444 8.947 1.558 −32.775 1.00 6.79 ATOM 2292 OG1 THR A 444 9.742 1.689 −31.590 1.00 6.54 ATOM 2293 CG2 THR A 444 7.983 2.703 −32.876 1.00 7.76 ATOM 2294 C THR A 444 9.215 −0.891 −32.618 1.00 6.16 ATOM 2295 O THR A 444 10.009 −1.092 −33.606 1.00 7.17 ATOM 2296 N GLY A 445 9.383 −1.559 −31.479 1.00 5.46 ATOM 2297 CA GLY A 445 10.462 −2.522 −31.258 1.00 5.45 ATOM 2298 C GLY A 445 10.664 −2.707 −29.754 1.00 5.51 ATOM 2299 O GLY A 445 9.863 −2.152 −28.975 1.00 5.68 ATOM 2300 N ILE A 446 11.708 −3.423 −29.411 1.00 4.79 ATOM 2301 CA ILE A 446 12.116 −3.597 −28.006 1.00 5.25 ATOM 2302 CB ILE A 446 12.767 −4.946 −27.768 1.00 5.78 ATOM 2303 CG1 ILE A 446 11.768 −6.077 −28.065 1.00 6.35 ATOM 2304 CD1 ILE A 446 12.373 −7.446 −28.204 1.00 6.58 ATOM 2305 CG2 ILE A 446 13.356 −5.072 −26.336 1.00 6.38 ATOM 2306 C ILE A 446 13.094 −2.465 −27.681 1.00 5.40 ATOM 2307 O ILE A 446 14.051 −2.197 −28.416 1.00 5.32 ATOM 2308 N VAL A 447 12.859 −1.768 −26.572 1.00 4.77 ATOM 2309 CA VAL A 447 13.778 −0.711 −26.129 1.00 5.62 ATOM 2310 CB VAL A 447 13.299 −0.114 −24.774 1.00 5.69 ATOM 2311 CG1 VAL A 447 14.394 0.769 −24.142 1.00 6.30 ATOM 2312 CG2 VAL A 447 12.025 0.632 −24.986 1.00 6.38 ATOM 2313 C VAL A 447 15.226 −1.247 −26.057 1.00 5.74 ATOM 2314 O VAL A 447 15.440 −2.328 −25.521 1.00 5.65 ATOM 2315 N ASP A 448 16.100 −0.427 −26.630 1.00 6.18 ATOM 2316 CA ASP A 448 17.519 −0.764 −26.680 1.00 6.89 ATOM 2317 CB ASP A 448 17.957 −0.846 −28.172 1.00 7.68 ATOM 2318 CG ASP A 448 19.408 −1.082 −28.355 1.00 10.42 ATOM 2319 OD1 ASP A 448 20.237 −0.899 −27.430 1.00 9.43 ATOM 2320 OD2 ASP A 448 19.801 −1.471 −29.524 1.00 11.71 ATOM 2321 C ASP A 448 18.246 0.315 −25.947 1.00 6.10 ATOM 2322 O ASP A 448 18.391 1.417 −26.384 1.00 6.60 ATOM 2323 N ALA A 449 18.637 −0.058 −24.711 1.00 5.94 ATOM 2324 CA ALA A 449 19.209 1.002 −23.826 1.00 6.13 ATOM 2325 CB ALA A 449 19.563 0.417 −22.503 1.00 6.67 ATOM 2326 C ALA A 449 20.440 1.632 −24.448 1.00 6.79 ATOM 2327 O ALA A 449 20.639 2.833 −24.321 1.00 7.24 ATOM 2328 N ALA A 450 21.304 0.814 −24.995 1.00 6.48 ATOM 2329 CA ALA A 450 22.575 1.362 −25.592 1.00 7.18 ATOM 2330 CB ALA A 450 23.479 0.268 −26.054 1.00 7.98 ATOM 2331 C ALA A 450 22.322 2.332 −26.711 1.00 6.36 ATOM 2332 O ALA A 450 22.888 3.445 −26.767 1.00 8.44 ATOM 2333 N ALA A 451 21.365 1.971 −27.602 1.00 7.06 ATOM 2334 CA ALA A 451 21.049 2.839 −28.700 1.00 6.77 ATOM 2335 CB ALA A 451 20.201 2.109 −29.744 1.00 7.45 ATOM 2336 C ALA A 451 20.370 4.134 −28.271 1.00 7.10 ATOM 2337 O ALA A 451 20.566 5.192 −28.814 1.00 7.94 ATOM 2338 N ALA A 452 19.477 4.036 −27.267 1.00 7.11 ATOM 2339 CA ALA A 452 18.844 5.166 −26.716 1.00 7.05 ATOM 2340 CB ALA A 452 17.762 4.738 −25.680 1.00 7.60 ATOM 2341 C ALA A 452 19.841 6.166 −26.098 1.00 6.88 ATOM 2342 O ALA A 452 19.726 7.334 −26.328 1.00 6.94 ATOM 2343 N VAL A 453 20.783 5.627 −25.291 1.00 7.38 ATOM 2344 CA VAL A 453 21.789 6.513 −24.705 1.00 8.19 ATOM 2345 CB VAL A 453 22.573 5.726 −23.682 1.00 9.35 ATOM 2346 CG1 VAL A 453 23.891 6.385 −23.283 1.00 10.30 ATOM 2347 CG2 VAL A 453 21.730 5.394 −22.445 1.00 8.50 ATOM 2348 C VAL A 453 22.659 7.155 −25.797 1.00 8.69 ATOM 2349 O VAL A 453 22.933 8.358 −25.674 1.00 10.09 ATOM 2350 N ALA A 454 23.022 6.384 −26.806 1.00 8.00 ATOM 2351 CA ALA A 454 23.758 6.955 −27.925 1.00 8.10 ATOM 2352 CB ALA A 454 24.087 5.895 −28.941 1.00 9.46 ATOM 2353 C ALA A 454 23.018 8.078 −28.583 1.00 9.26 ATOM 2354 O ALA A 454 23.567 9.156 −28.916 1.00 10.25 ATOM 2355 N ALA A 455 21.713 7.924 −28.779 1.00 8.78 ATOM 2356 CA ALA A 455 20.900 8.954 −29.382 1.00 8.33 ATOM 2357 CB ALA A 455 19.513 8.380 −29.804 1.00 9.67 ATOM 2358 C ALA A 455 20.694 10.155 −28.531 1.00 9.32 ATOM 2359 O ALA A 455 20.446 11.260 −29.059 1.00 12.53 ATOM 2360 N ALA A 456 20.815 10.010 −27.218 1.00 9.27 ATOM 2361 CA ALA A 456 20.657 11.137 −26.325 1.00 10.15 ATOM 2362 CB ALA A 456 20.200 10.564 −24.993 1.00 10.18 ATOM 2363 C ALA A 456 22.011 11.833 −26.080 1.00 12.46 ATOM 2364 O ALA A 456 22.023 12.825 −25.388 1.00 15.16 ATOM 2365 N SER A 457 23.122 11.287 −26.551 1.00 12.94 ATOM 2366 CA SER A 457 24.543 11.722 −26.369 1.00 15.79 ATOM 2367 CB SER A 457 25.042 12.858 −27.243 1.00 21.74 ATOM 2368 OG SER A 457 24.369 12.859 −28.485 1.00 22.46 ATOM 2369 C SER A 457 25.442 10.593 −25.978 1.00 14.19 ATOM 2370 O SER A 457 25.112 9.837 −24.931 1.00 16.08 HETATM 2371 CA CA C 1 22.679 −5.208 −5.643 1.00 6.61 HETATM 2372 CA CA C 2 −2.136 6.605 5.488 1.00 7.73 HETATM 2373 CA CA C 3 1.597 5.590 4.829 1.00 6.15 HETATM 2374 CA CA C 4 2.532 7.587 −26.583 1.00 6.29

In Table 1, A: atom; B: atomic number; C: atomic name; D: residue name; E: chain name; F: residue number; G: x-axis information; H: y-axis information; I: z-axis information; J: occupancy; and K: temperature factor.

In another aspect, the present invention is directed to a method for crystallizing a cold-adapted protease derived from Pseudoalteromonas arctica PAMC 21717 or a recombinant cold-adapted protease obtained by expressing a gene encoding the cold-adapted protease in E. coli, wherein the method comprises crystallizing at 20° C. using a protein solution containing 20 mM Tris-HCl (pH 8.0) and 150 mM NaCl, and a preservative solution containing 0.1 M sodium acetate (pH 4.4) and 3 M sodium chloride.

This crystallization step is generally performed using x-ray crystallography, and various crystallization processes should be performed as a pretreatment step prior to x-ray crystallography. In an embodiment of the present invention, a conventional method based on concentration equilibrium may be used as this crystallization method. For example, a vapor equilibrium method, a sitting- or handing-drop vapor diffusion method or a dialysis method (continuous or batch) may be used. In an example of the present invention, the sitting method was used to crystallize and concentrate the cold-adapted protease.

In still another aspect, the present invention is directed to a method for preparing a cold-adapted protease, the method comprises: (a) fed-batch-culturing Pseudoalteromonas arctica PAMC 21717 in a medium containing skim milk, tryptone, Fe(C₆H₅O₇), NaCl, MgCl₂, Na₂SO₄, CaCl₂, NaHCO₃ and KBr, to produce a cold-adapted protease (W-pro21717); and (b) recovering the produced cold-adapted protease.

In yet another aspect, the present invention is directed to a method for preparing an enzyme-producing recombinant microorganism that expresses a cold-adapted protease, the method comprises: (a) performing PCR with a set of primers of SEQ ID NOs: 7 and 8, to obtain a gene encoding a cold-adapted protease (W-pro21717) derived from Pseudoalteromonas arctica PAMC 21717; (b) inserting the gene into a recombinant expression vector pEXP5-CT/TOPO; and (c) transforming host E. coli with the recombinant expression vector.

As used herein, the term “vector” refers to a DNA construct containing a DNA sequence operably linked to a suitable control sequence capable of expressing the DNA in a suitable host. The vector may be a plasmid, a phage particle, or simply a potential genomic insert. Once transformed into a suitable host, the vector may replicate and function independently of the host genome, or may, in some instances, integrate into the genome itself. In the present specification, plasmid and vector are sometimes used interchangeably as the plasmid is the most commonly used form of vector at present. For the purpose of the present invention, a plasmid vector is preferably used. A typical plasmid vector that can be used for this purpose has: (a) a replication origin by which replication occurs efficiently such that several hundred plasmid vectors per host cell are created; (b) an antibiotic-resistant gene by which host cells transformed with the plasmid vector can be selected; and (c) restriction enzyme digestion sites into which foreign DNA fragments can be inserted. Even if suitable restriction enzyme digestion sites are not present in the vector, the use of a conventional synthetic oligonucleotide adaptor or linker enables easy ligation between the vector and a foreign DNA.

After ligation, the vector should be transformed into suitable host cells. The transformation can be easily achieved using the calcium chloride method described in section 1.82 of Sambrook, et al., supra. Alternatively, electroporation (Neumann, et al., EMBO J., 1:841, 1982) may also be used for the transformation of such cells.

For the overexpression of the gene according to the present invention, an expression vector known in the art may be used.

As well-known to the art, in order to increase the expression level of a transformed gene in a host cell, the gene should be operably linked to transcription and translation control sequences which can function in a selected expression host. Preferably, the corresponding gene and expression control sequence are contained in a single expression vector comprising both a bacterial selectable marker and a replication origin.

The host cell transformed with the above-described recombinant vector constitutes another aspect of the present invention. As used herein, the term “transformation” refers to introducing DNA into a host cell so that the DNA is replicable by either as a chromosomal integrant or as an extrachromosomal element.

Of course, it should be understood that all vectors do not function equally to express the DNA sequence of the present invention. Likewise, all host cells do not function equally for the same expression system. However, any person skilled in the art may properly select a vector, an expression control sequence and a host cell without departing from the scope of the present invention and without undue experimentation. For example, in selection of a vector, a host cell should be taken into consideration, because the vector should be replicated therein. Also, the replication number of a vector, the capability to control the replication number, and the expression of other proteins encoded by the vector, for example, an antibiotic marker, should be taken into consideration.

The recombinant vector that is used in the present invention is preferably a pDOC vector comprising pro21717-I gene introduced in pEXP5-CT/TOPO, and more preferably pDOC131. The recombinant microorganism is preferably E. coli, such as E. coli DH5α, E. coli JM101, E. coli K12, E. coli W3110, E. coli X1776, E. coli XL-1 Blue (Stratagene), E. coli BL21 or the like.

In a further aspect, the present invention is directed to a method for preparing a cold-adapted protease, the method comprises: (a) culturing the recombinant microorganism of claim 6 in a medium containing glucose, KH₂PO₄, (NH₄)₂PO₄, citric acid, MgSO₄7H₂O, thiamine, an antibiotic and a trace metal element; (b) expressing a cold-adapted protease in the recombinant microorganism to a pH-stat batch culture while supplying a predetermined amount of a medium containing glucose, a yeast extract, (NH₄)₂PO₄, MgSO₄7H₂O and an antibiotic when the pH and DO of the medium is increased; and (c) recovering the expressed cold-adapted protease.

In the present invention, the protease is characterized in that it exhibits enzymatic activity under the conditions of 0 to 60° C. and pH 5.0 to 11.0, and is purified by a Sephacryl S-100 column using an unfolding buffer (pH 8.5; 8 M urea, 50 mM mercaptoethanol and 20 mM Tris-HCl), a refolding buffer (20 mM Tris-HCl, 100 mM NaCl, 20 mM CaCl₂ and 0.05% Tween 20), and a dialysis step.

In a still further aspect, the present invention is directed to a disinfectant composition for a surgical or therapeutic device, a detergent composition, a feed additive composition, a food additive composition, and a fiber or leather processing composition, the composition containing the cold-adapted protease or a crystal of the cold-adapted protease as an active ingredient.

The protease according to the present invention may be used as a disinfectant for a surgical or therapeutic device. The surgical or therapeutic device may be a medical device that is to be inserted into the body, such as an endoscope, a catheter or the like.

The protease according to the present invention may be used as a detergent composition. The detergent composition may be used in various applications, including laundry, dish washing, contact lens washing, false-tooth washing, and the like. The detergent composition may comprise, in addition to the protease of the present invention, other enzyme components and additives. The detergent composition of the present invention may comprise at least one surfactant, and the surfactant an anionic surfactant, a non-ionic surfactant, a catonic surfactant, an amphoteric or zwitterionic surfactant, or a mixture of two or more thereof. In addition, the detergent composition of the present invention may further comprise other detergent components known in the art, for example, an abrasive agent, a bleaching agent, a surface active agent, an anti-corrosive agent, a metal ion sequestering agent, an anti-stain reposition agent, a stabilizer for a perfume, an enzyme and a bleaching agent, a formulation aid, an optical brightening agent, a foam booster, a chelating agent, a filling agent, a fabric softener, or the like. In addition, the detergent composition of the present invention may be formulated in any convenient form such as powder, liquid or the like.

The protease according to the present invention may be a feed additive, a food additive or a pharmaceutical composition. The protease can be used in applications, including a food softener, a meat enhancer, an oil and fat separating agent, and the like. In addition, it can be used as a digestive enzyme agent for alleviating gastrointestinal disorders, digestive abnormalities, and abnormal diseases after gastrointestinal surgery, a thrombolytic agent that acts directly on thrombi to dissolve fibrin, an anti-inflammatory enzyme that acts as an in vivo defense system against foreign toxic substances to remove inflammatory substances or necrotic tissue, or an anti-inflammatory agent for post-surgery or post-trauma edema. The pharmaceutical composition according to the present invention comprises a pharmaceutically acceptable carrier in a mixture of the protease of the present invention. Pharmaceutical formulations are well known in the art, and the pharmaceutical composition comprising the protease can be easily formulated by those skilled in the art. The pharmaceutical composition may be administered parenterally (e.g., intravenously, subcutaneously or intramuscularly). Preferably, it is administered intravenously. The pharmaceutical composition is preferably used in the form of a sterile aqueous solution containing a sufficient amount of a salt, glucose or dextrose, which makes the solution isotonic. For oral administration, the pharmaceutical composition may be used in the form of tablets, capsules, lozenges, troches, powder, syrup, elixirs, solutions or suspensions, and may comprise various disintegrants such as starch, and lubricants.

The protease according to the present invention may be used as a fiber or leather processing agent, and may be used for removal of rubbery material from silk fiber, soaking of leather, etc.

EXAMPLES

Hereinafter, the present invention will be described in further detail with reference to examples. It will be obvious to a person having ordinary skill in the art that these examples are illustrative purposes only and are not to be construed to limit the scope of the present invention.

Example 1 Examination of Protease (W-Pro21717) Derived from Pseudoalteromonas arctica PAMC 21717

1-1: Culture and Examination of Production of W-Pro21717

Pseudoalteromonas arctica PAMC 21717 was cultured in 2% glucose-containing ZoBell medium (per liter, 5 g peptone, 1 g yeast extract, 0.01 g FePO₄, 750 ml seawater, and 250 ml distilled water) under three temperature conditions (5° C., 15° C. and 25° C.), and was then cultured at 25° C. for 1 day.

In order to measure the degradation activity of W-Pro21717, the cultured PAMC 21717 cells were centrifuged at 4° C. at 12,000×g for 30 minutes, and the supernatant was mixed with 50 mM sodium phosphate buffer (pH 7.6) containing 0.65% azocasein substrate, and was allowed to at 25° C. for 1 hour, and then additionally allowed to react at 37° C. for 1 hour after adding 110 mM trichloroacetic acid thereto. The reaction solution was centrifuged, and then filtered through a filter having a pore size of 0.45 μm, and the filtrate was mixed and reacted with Folin & Ciocalteu's phenol solution and 500 mM sodium carbonate solution at 37° C. for 30 minutes. Next, the enzymatic activity of the reaction product was measured at 660 nm based on an L-tyrosine standard curve, and an amino acid production of 1 nmole/min/mg at 25° C. was defined as the activity of 1 unit of protease.

As a result, it was shown that the maximum activity of W-Pro21717 was 2.7 U/ml at 15° C., 2.1 U/ml at 5° C., and 1.4 U/ml at 25° C., suggesting that the optimum temperature for the production of the protease was 15° C.

1-2: Zymogram Analysis and Kind of W-Pro21717

Pseudoalteromonas arctica PAMC 21717 was cultured at 15° C. for 96 hours, and then the enzyme protein isolated from the cells was electrophoresed on 10% SDS-PAGE gel comprising 0.3% skim milk and 1% gelatin. The PAGE gel was reacted with a denaturing buffer (27 g/L Triton X-100), and then reacted with a developing buffer (per liter of distilled water, 1.21 g Tris base, 6.3 g Tris-HCl, 11.7 g NaCl, 0.74 g CaCl₂, 0.02% Brij35) for 30 minutes, after the gel was stained with Coomassie blue, and the degradation activity of the protein was examined by zymogram analysis.

As a result, it was shown that two proteins (37 kDa and kDa) found in the zymogram contained an amino acid sequence of GAQNSSWH in their N-terminal end, and this amino acid sequence was consistent with the sequences of many serine proteases derived from Pseudoalteromonas spp. The 37 kDa and 74 kDa proteins derived from Pseudoalteromonas arctica PAMC 21717 were named “W-Pro21717” (FIG. 1), and the 74 kDa protein having a higher activity may be a homodimer of the 37 kDa protein.

The kind of W-Pro21717 was analyzed by reactions with various protease inhibitors.

As a result, as can be seen in Table 2 below, the activity of W-Pro21717 was inhibited by PMSF and HgCl₂, but was not influenced by other cysteine protease inhibitors. Also, the activity of W-Pro21717 was slightly inhibited by the metalloprotease inhibitor EDTA. Thus, it was found that a disulfide bond is important in enzymatic activity and that W-Pro21717 is a serine-based protease.

TABLE 2 Concentration Residual Inhibitor (mM) activity (%) None — 100 EDTA 1 75 ± 9 PMSF 1 40 ± 8 HgCl₂ 1 23 ± 8 Iodoacetamide 1 94 ± 3 APMA 1 131 ± 2  N-Ethylmaleimide 1 107 ± 8  Leupeptin 1 98 ± 2 N^(a)-p-tosyp-L-lysine 1 137 ± 13 chloromethyl ketone

Example 2 Characterization of Protease (W-Pro21717) Derived from Pseudoalteromonas arctica PAMC 21717

2-1: Activity and Stability of W-Pro21717 with Temperature and pH

A temperature optimal for the activity of W-Pro21717 was analyzed in the temperature range of 0 to 60° C., and the optimum pH was analyzed by 1-hour reactions in 50 mM sodium acetate (pH 2.0-6.0), 50 mM potassium phosphate (pH 7.0-10.0) and 50 mM sodium tetraborate (pH 10.0). As a control, 3 μg of the commercial protease Carlsberg subtilisin was used.

As a result, it was shown that W-Pro21717 showed the highest activity at a temperature of 30˜40° C. and that a temperature optimal for the activity of W-Pro21717 was 40° C. (FIG. 2A). Also, the control subtilisin Carlsberg showed the optimum activity at 60° C. In addition, at about or, W-Pro21717 showed an activity of 30%.

After a reaction at 40° C. for 1 hour, W-Pro21717 showed a decrease in activity of 30% (FIG. 2B), but subtilisin Carlsberg showed a decrease in activity of 80%. Thus, it was found that W-Pro21717 is a cold-adapted protease having a high low-temperature activity and stability.

W-Pro21717 showed high activity at a pH of 8.0-9.5, and showed the highest activity at a pH of 9.0 (FIG. 2C). In addition, it showed stability in a relatively wide pH range (pH 5.0-10.0), but lost its activity at a pH lower than 5.0 and a pH higher than 10.0 (FIG. 2D).

2-2: Activity and Stability of W-Pro21717 in the Presence of Metal Ions and Surfactants

In order to analyze the influence of metal ions and detergents, W-Pro21717 was allowed to react with a standard buffer containing 1 mM BaCl₂, CuSO₄, MgSO₄, CaCl₂, ZnSO₄, FePO₄, KCl, NaCl, N₂SO₄, sodium linear alkylbenzene sulfonate (LAS) or SDS on ice for 1 hour. In addition, the compatibility of W-Pro21717 with a commercial detergent (LG Household & Health Care, Korea; containing subtilisin Carlsberg) was analyzed using skim milk at temperatures of 10° C. and 30° C.

As a result, it was shown that CuSO₄ increased the activity of W-Pro21717 by about 60%, and Ba²⁺, Mg²⁺, Ca²⁺, Zn²⁺, Fe²⁺, K⁺ and Na²⁺ did not influence the activity of W-Pro21717 (FIG. 3).

In addition, W-Pro21717 showed sufficient stability in the presence of the standard components (Na₂SO₄ and LAS) of general commercial detergents. Also, the results of measurement of the compatibility of W-Pro21717 with the general detergent indicated that W-Pro21717 showed the activities of 54.9±2.2 U/mg and 20.7±3.4 U/mg at 30° C. and 10° C., respectively (FIG. 4).

2-3: Substrate Specificity of W-Pro21717

The substrate specificity of W-Pro21717 was analyzed using 5 mM of each of the following 7 different synthetic peptides: N-succinyl-Ala-Ala-Val-p-nitroanilide(AAV), N-succinyl-Ala-Ala-Pro-Leu-p-nitroanilide(AAPL), N-succinyl-Ala-Ala-Ala-p-nitroanilide(AAA), N-succinyl-Gly-Gly-Phe-p-nitroanilide(GGF), N-succinyl-Thr-Leu-Val-p-nitroanilide(TLV), N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide(AAPF), and N-succinyl-Ala-Ala-Val-Ala-p-nitroanilide(AAVA). W-Pro21717 was allowed to react with a standard buffer containing 0.83 mM substrate at 25° C. for 10 minutes, and then the relative activity thereof was measured at 410 nm.

As a result, as can be seen in Table 3 below, W-Pro21717 showed high activity for AAPF (100%) and AAPL (66.5%), and subtilisin Carlsberg also had substrate specificities of 100% and 68.3%, which were similar to those of W-Pro21717.

TABLE 3 Comparison of specificity for various substrates between W-Pro21717 and subtilisin Carlsberg Substrate Relative activity (%) Substrate W-Pro21717 Subtilisin Carlsberg AAV 3.1 ± 2.3 2.6 ± 1.3 AAPL 44.3 ± 1.1  66.5 ± 0.1  AAA 2.1 ± 1.1 1.5 ± 0.5 GGF 4.7 ± 1.7  0 TLV 6.7 ± 2.4 4.0 ± 3.7 AAPF 100 100 AAVA 8.7 ± 1.5 11.7 ± 2.3 

Example 3 Culture Optimization for Production of Cold-Adapted Protease (W-Pro21717) Derived from Pseudoalteromonas arctica PAMC 21717

In this Example, for statistical optimization of the production of W-Pro21717, the optimum conditions for the production of W-Pro21717 derived from Pseudoalteromonas arctica PAMC 21717 were established through fed-batch culture at 15° C. For optimization of carbon, nitrogen and mineral media, a Plackett-Burman design was used.

As a result, fructose was selected as a carbon source, and it was shown that the activity of the enzyme was 8.0 U/L in a skim milk-containing medium and was 3.4 U/L in a medium containing no skim milk, suggesting that skim milk increased the activity of the enzyme by 2.3 times (FIGS. 5A and 5B). Nitrogen sources showed no difference in the activity of the enzyme, but tryptone was selected as a nitrogen source (FIG. 5C), and it was shown that the activity of the enzyme was increased by 3.4 times when Fe(C₆H₅O₇), NaCl, Na₂SO₄ and KCl as mineral sources were contained in amounts of 0.1, 24.8, 4.4 and 0.7 g/L, respectively (FIG. 6).

Fed-batch culture was performed in a medium composition containing 10 g/L skim milk, 0.6 g/L tryptone, 0.1 g/L Fe(C₆H₅O₇), 24.8 g/L NaCl, 5.9 g/L MgCl₂, 4.4 g/L Na₂SO₄, 1.8 g/L CaCl₂, 0.16 g/L NaHCO₃ and 0.08 g/L KBr at 15° C. The activity of W-Pro21717 induced in the culture medium was 53.4 U/L, which was at least 15 times higher than that in the non-optimized culture medium (FIGS. 7A and 7B).

Example 4 Construction of Recombinant Protease (R-Pro21717) Based on Genetic Information of W-Pro21717

4-1: Vector Containing W-Pro21717-Encoding Gene

In this Example, the genomic library of Pseudoalteromonas arctica PAMC 21717 was constructed using the CopyControl Fosmid Library Production Kit (Epicentre). Among 13 clones having proteolytic activity, EPI-P38 was selected and cleaved with 8 different restriction enzymes, after which it was introduced into a pUC19 vector, thereby obtaining the clone Rosetta-P38-4 having proteolytic activity.

The W-Pro21717 gene encoding the cold-adapted protease was amplified with the following primers, and then inserted into the expression vector pEXP5-CT/TOPO, thereby constructing and selecting plasmids having excellent proteolytic activity.

The W-Pro21717 gene was amplified by PCR using the chromosomal DNA of the Pseudoalteromonas arctica PAMC 21717 strain as a template and a primer set of SEQ ID NOs: 1 and 2 for pDOC122, a primer set of SEQ ID NOs: 3 and 6 for pDOC125, a primer set of SEQ ID NOs: 5 and 6 for pDOC128, or a primer set of SEQ ID NOs: 7 and 8 for pDOC131.

pDOC122 SEQ ID NOs: 1: 5′-ATGACAACAAGTAAAACTTTTAAAAGATGCGC-3′ SEQ ID NOs: 2: 5′-CACTTAGCGGACAATACCAACCG-3′ pDOC122 SEQ ID NOs: 3: 5′-ATGCAATCAGTTTCAAGTTCAATGGC-3′ SEQ ID NOs: 4: 5′-CACTTAGCGGACAATACCAACCG-3′ pDOC128 SEQ ID NOs: 5: 5′-ATGACAACAAGTAAAACTTTTAAAAGATGCGC-3′ SEQ ID NOs: 6: 5′-GCAGCTGTTGCAGCAGCAAGT-3′ pDOC131 SEQ ID NOs: 7: 5′-ATGCAATCAGTTTCAAGTTCAATGGC-3′ SEQ ID NOs: 8: 5′-GCAGCTGTTGCAGCAGCAAGT-3′

4-2: Expression and Purification of R-Pro21717

In this Example, the function of the recombinant protein R-Pro21717 of the protease (W-Pro21717) derived from Pseudoalteromonas arctica PAMC 21717 was analyzed.

The four plasmids constructed in Example 4-1 were overexpressed, and the inclusion body (IB) of R-Pro21717 was analyzed by zymogram on 10% SDS-PAGE gel.

As a result, the protein expression of 72.6 kDa pDOC122 and 49.7 kDa pDOC128 was not observed. However, the expression of 69.9 kDa pDOC125 and 47 kDa pDOC131 was observed (FIG. 8A). pDOC131 that showed a significantly high proteolytic activity in zymogram analysis was finally selected.

pDOC131-containing E. coli BL21 star(DE3)pLysS was cultured in ampicillin-containing LB medium. When an OD₆₀₀ of 1.0 was reached, IPTG was added to the cells, which were then cultured for 24 hours, thereby inducing the expression of the R-Pro21717 recombinant protein. The cells were centrifuged, and the unfolded IB (inclusion body) was washed with an unfolding buffer. The unfolded protein solution was diluted with a refolding buffer, and it was dialyzed and purified by FPLC. The purified protein was the Pro21717 recombinant protein having proteolytic activity.

The expression of the insoluble portion other than water-soluble portion of the recombinant protein could be observed, the shift of the size occurred during the refolding process. The protein changed from 50 kDa to 37 kDa after 48 hours of refolding and changed to 63 kDa after 72 hours of refolding (FIG. 8B). It can be seen that the enzymatic activity of W-Pro21717 was 42.1±3.5 U/mg, but the enzymatic activity of the R-Pro21717 recombinant protein was 116.4±2.7 U/mg, which was 2.8 times higher than that of W-Pro21717. The refolded protease was purified by size exclusion chromatography, thereby constructing an R-Pro21717 recombinant protein showing an enzymatic activity of 268.6±7.5 U/mg, which was 6.4 times higher than that of W-Pro21717 (FIG. 8C).

Example 5 Activity and Stability of Recombinant Protease (R-Pro21717)

5-1: Influence of Temperature and pH

In this Example, the activity and stability of R-Pro21717 at low temperatures and high pHs were analyzed.

In order to determine a temperature optimal for the activity of the protease, R-Pro21717 was allowed to react at varying temperatures of 0˜70° C., and then analyzed. The stability of R-Pro21717 against temperature was analyzed by allowing R-Pro21717 at varying temperatures of 0˜70° C. for 1 hour, and then allowing R-Pro21717 to react a 0.65% azocasein-containing standard buffer at 30° C. for 30 minutes. As a control enzyme, Carlsberg subtilisin was used.

In order to determine a pH optimal for the activity of the protease, R-Pro21717-containing buffers having varying pHs were allowed to react at 30° C., and then analyzed. The stability of R-Pro21717 against pH was analyzed by allowing R-Pro21717-containing buffers having varying pHs to react on ice for 1 hour, and then reacting R-Pro21717 in 0.65% azocasein-containing standard buffer at 30° C. for 30 minutes. Carlsberg subtilisin was used as a control enzyme and analyzed at a reaction temperature of 40° C.

As a result, it was shown that the R-Pro21717 protease showed the highest activity at a temperature ranging from 30 to 40° C., and a temperature optimal for the activity of the R-Pro21717 protease was 30° C., and at or, the R-Pro21717 protease showed a proteolytic activity of 50% of the highest activity at 30° C. (FIG. 9A). However, the control enzyme Carlsberg subtilisin showed the highest activity at 60° C., and did not show proteolytic activity at or compared to 60° C. at which it showed the highest activity. In addition, it was shown that the stability of the R-Pro21717 protease against temperature decreased at 20° C. or higher (FIG. 9B), and the control enzyme Carlsberg subtilisin showed a decrease in enzymatic activity at 50° C. or higher.

In addition, it was shown that the R-Pro21717 protease showed the highest activity at a pH of 9.0 (FIG. 9C) and that a pH optimal for the stability of the enzyme was 5.0-11.0 (FIG. 9D), and the control enzyme Carlsberg subtilisin, a basic enzyme, also showed the same results as those of R-Pro21717.

5-2: Influence of Metal Ions and Surfactants

In this Example, the activity and stability of the R-Pro21717 protease in the presence of metal ions and surfactants were analyzed.

The analysis was performed by adding R-Pro21717 to a standard buffer containing CaCl₂, CuSO₄, NaCl, N₂SO₄, LAS, SDS, PMSF or 1% H₂O₂, allowing the mixture to react on ice for 1 hour, and then adding 0.65% azocasein thereto (FIG. 10).

As a result, it was shown that the activity and stability of the R-Pro21717 protease in the presence of metal ions and surfactants were better than those of general detergent enzymes, like the control enzyme Carlsberg subtilisin.

5-3: Substrate Specificity

In this Example, the activity of the R-Pro21717 protease in the presence of synthetic peptide substrates was analyzed. As a result, it was shown that the R-Pro21717 protease showed activity similar to that of the control enzyme Carlsberg subtilisin, and showed relatively low activities in the presence of complex substrates including collagen and keratin (Table 4).

Accordingly, it was found that the cold-adapted protease derived from Pseudoalteromonas arctica PAMC 21717 according to the present invention shows high activity at low temperatures, and securely maintains its enzymatic activity even in the presence of various metal ions and surfactants that are contained in detergent compositions. This suggests that the cold-adapted protease derived from Pseudoalteromonas arctica PAMC 21717 can be used in various industrial applications.

TABLE 4 Substrate Relative activity (%) Substrate R-Pro21717 Subtilisin Carlsberg Macro Collagen 8.3 ± 2.8  6.7 ± 1.2 molecule Macro Keratin 14.1 ± 0.3  30.2 ± 5.6 molecule Azocasein 100 100 Skim milk 41.7 ± 2.5  67.7 ± 5.7 Oligo AAV 2.4 ± 1.4  7.9 ± 3.9 molecule Oligo AAPL 41.7 ± 0.2   57.1 ± 10.1 molecule AAA 2.1 ± 0.8 10.2 ± 1.9 GGF 11.2 ± 7.4  16.2 ± 2.1 TLV 3.8 ± 0.1 11.9 ± 1.1 AAPF 100 100 AAVA 6.4 ± 2.1 12.9 ± 0.6

Example 6 Culture Optimization for Production of Recombinant Protease (R-Pro21717)

In this Example, for optimization of the production of the recombinant protease R-Pro21717, the optimum conditions for the production of R-Pro21717 in the recombinant microorganism prepared in Example 4 were established through batch-type culture and fed-batch culture.

Culture was performed in a medium containing glucose, KH₂PO₄, (NH₄)₂PO₄, citric acid, MgSO₄7H₂O, thiamine, an antibiotic and a trace metal element at 37° C., and then when the pH and DO of the medium rose due to complete consumption of glucose, pH-stat fed-batch was performed for at least 15 hours while a predetermined amount of a medium containing glucose, a yeast extract, (NH₄)₂PO₄, MgSO₄7H₂O and an antibiotic was fed, thereby expressing a cold-adapted protease. In this way, the production of R-Pro21717 was optimized.

Example 7 Crystallization and Structural Analysis of Cold-Adapted Protease

A purified protease was concentrated to a concentration of 250 mg/ml, and then crystallized at 20° C. using the sitting drop vapor diffusion method. For crystallization, a protein solution 0.81 μl of a protein solution (containing 250 mg/ml protein, 20 mM Tris-HCl (pH 8.0) and 150 mM NaCl) and 0.81 μl of a preservative solution (0.1 M sodium acetate (pH 4.4) and 3 M sodium chloride) were used. The single crystal grew in the form of needles so that the longest axis had a length of about 0.5 mm or less after 2 days. For a cryogenic experiment, the protease crystal was immersed in paratone oil, and after 2 minutes, immersed in liquid nitrogen. X-ray diffraction data were analyzed at a resolution of 1.4 Å at the 7A beamline of the Pohang Accelerator Laboratory (Pohang, Republic of Korea), and indexed using the HKL2000 program.

As a result, it was shown that the protease crystal had a space group of P2₁2₁2₁ with unit cell dimensions of a=47.9 Å, b=74.6 Å and c=83.0 Å. The protease crystal had the atomic coordinates shown in Table 5 below and Table 1 above.

In addition, the structure of the crystal was analyzed by a molecular replacement method using the MOLREP program and an AprV2 structure (PDB code 3LPA) as a template, and the refinement of the structure was performed using REFMAC5 and the Coot program.

The overall structure of the protease has a subtilisin-like fold and is composed of ten α-helices which surround the central 6 β-strands and two β-strands. The enzymatic active site is composed of a catalytic triad consisting of three residues (Asp159, His218 and Ser399). Thus, it could be found from the structural information of the protease that the protease is a serine-based protease. In addition, the protease has four calcium ion bonds that assist in the stabilization of the protease (FIG. 11).

TABLE 5 Data set X-ray source Beamline 7A, PAL Space group P2₁2₁2₁ Wavelength (Å) 0.97934 Resolution range (Å) 30.00-1.40 (1.42-1.40) No. of observed reflections 804441 No. of unique reflections 58245 (2849) R_(sym) ^(a)(%) 6.6 (15.0) Average I/σ 69.0 (36.3) Completeness (%) 98.0 (98.2) Multiplicity 13.8 (14.3) Refinement Resolution (Å) 55.47-1.40 (1.44-1.40) No. of reflections in working set 55253 (4010) No. of reflections in test set 2939 (207) No. of residues 339 No. of water molecules 579 No. of calcium ions 4 R_(cryst) ^(b)total(%) 13.72 (14.3) R_(free) ^(c)total(%) 16.09 (16.5) R.m.s. bond length (Å) 0.028 R.m.s. bond angle (°) 2.42 Average B value (Å²) 10.769 ^(a) R_(sym) = Σ 

 <I> − I 

 /Σ<I>. ^(b) R_(cryst) = Σ|Fo| − |Fc| 

 /Σ|Fo|. ^(c) R_(free) calculated with 10% of all reflections excluded from refinement stages using high resolution data. Values in parentheses refer to the highest resolution shells.

Although the present invention has been described in detail with reference to the specific features, it will be apparent to those skilled in the art that this description is only for a preferred embodiment and does not limit the scope of the present invention. Thus, the substantial scope of the present invention will be defined by the appended claims and equivalents thereof.

[Deposit of Microorganisms]

Depository Institution: Korea Research Institute of Bioscience and Biotechnology;

Accession Number: KCTC12482BP;

Deposit Date: Sep. 3, 2013. 

What is claimed is:
 1. A method for preparing a cold-adapted protease, the method comprising: (a) fed-batch-culturing Pseudoalteromonas arctica PAMC 21717 in a medium containing skim milk, tryptone, Fe(C₆H₅O₇), NaCl, MgCl₂, Na₂SO₄, CaCl₂, NaHCO₃ and KBr, to produce a cold-adapted protease (W-pro21717); and (b) recovering the produced cold-adapted protease.
 2. An enzyme-producing recombinant microorganism that expresses a cold-adapted protease (R-pro21717), in which a recombinant vector containing an amplification product obtained by PCR using a set of primers of SEQ ID NOs: 7 and 8 is introduced.
 3. The enzyme-producing recombinant microorganism of claim 2, wherein the microorganism is E. coli.
 4. A method for preparing a cold-adapted protease (R-pro21717), the method comprising: (a) culturing the recombinant microorganism of claim 2 in a medium containing glucose, KH₂PO₄, (NH₄)₂PO₄, citric acid, MgSO₄7H₂O, thiamine, an antibiotic and a trace metal element; (b) expressing a cold-adapted protease in the recombinant microorganism to a pH-stat batch culture while supplying a predetermined amount of a medium containing glucose, a yeast extract, (NH₄)₂PO₄, MgSO₄7H₂O and an antibiotic when the pH and DO of the medium is increased; and (c) recovering the expressed cold-adapted protease (R-pro21717). 